FastQCFastQC Report
Fri 10 Feb 2017
ERR1630510.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630510.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences869931
Sequences flagged as poor quality0
Sequence length43
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT40130.4613009537538034No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT34840.400491533236544No Hit
GCCCAGGGCCAGGACGATGGACAGCGCAGCCAGCGCGCACTGG32700.3758918810802236No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT31260.35933884411522293No Hit
GATATGGACAATACTAGTTAAGAAAGCTAACAGGATGTGAAAG30820.35428097170925055No Hit
GCCTGGGACAGATCTTCAGGTTCCAGGGCATCATTCTCCGTCT27960.32140480107042974No Hit
GTCCCAGGCTGCTGAGCAGGATGAAATGAGGCTTGAGCTGCAG24150.2776082241005321No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23800.2735849165048722No Hit
GCCCTGGGCTGTGTCACCGGCGCTCCCTCGGACCCCAGACTCC23170.26634296283268444No Hit
GTCTGAACCCAACCAGACGGAGAATGATGCCCTGGAACCTGAA22950.2638140266296982No Hit
ACGATGGACAGCGCAGCCAGCGCGCACTGGAGGCGGCAGGACA20040.23036309776292602No Hit
GGTTCAGACAGCAGCTCTGCCAAGAAGTACTTGGCCAGTTCCT19380.2227762891539674No Hit
GGATGAAATGAGGCTTGAGCTGCAGAGATCTGCTAACTCAAAC19210.22082211117893258No Hit
AGCAGGAACTGGCCAAGTACTTCTTGGCAGAGCTGCTGTCTGA18910.2173735618112241No Hit
GCCAAGAAGTACTTGGCCAGTTCCTGCTTCCCCGCGGCAGCAG18830.21645394864650186No Hit
GGCCAGGACGATGGACAGCGCAGCCAGCGCGCACTGGAGGCGG18640.21426986738028647No Hit
CTTCTTGGCAGAGCTGCTGTCTGAACCCAACCAGACGGAGAAT18170.2088671400375432No Hit
ATTTCATCCTGCTCAGCAGCCTGGGACAGATCTTCAGGTTCCA17800.20461392915070276No Hit
AGCTAACAGGATGTGAAAGTCTTCCAGAAGAAATTCTTGCAGC17290.19875139522559834No Hit
CTTTAGGAGCGAGGTTCGGAGCCATCGCTGCTGCCTGCTGATC16890.19415332940198707No Hit
GTCCATCGTCCTGGCCCTGGGCTGTGTCACCGGCGCTCCCTCG16880.1940383777563968No Hit
CTGCTGATCCGCGCCTAGAGTTTGACCAGCCACTCTCCAGCTC16690.1918542964901814No Hit
GTCCTGGCCCTGGGCTGTGTCACCGGCGCTCCCTCGGACCCCA16250.18679642408420896No Hit
GGACAATACTAGTTAAGAAAGCTAACAGGATGTGAAAGTCTTC15770.1812787450958754No Hit
GTTCCTGCTTCCCCGCGGCAGCAGCCAGGGACTTCTGCAGAAA15510.17829000231052808No Hit
GATCAGCAGGCAGCAGCGATGGCTCCGAACCTCGCTCCTAAAG14580.1675994992706318No Hit
CCCTCGGACCCCAGACTCCGTCAGTTTCTGCAGAAGTCCCTGG13700.15748375445868695No Hit
AGTTAAGAAAGCTAACAGGATGTGAAAGTCTTCCAGAAGAAAT13630.15667909293955498No Hit
ATACTAGTTAAGAAAGCTAACAGGATGTGAAAGTCTTCCAGAA13270.1525408336983048No Hit
GAGTTTGACCAGCCACTCTCCAGCTCGGCTTTCGCGGCGCCGA13020.14966704255854776No Hit
GTCCATATCAGACCTCTGATCCCTCGCCCCCACACCCCATCTC12820.1473680096467421No Hit
TAGCAGATCTCTGCAGCTCAAGCCTCATTTCATCCTGCTCAGC12300.1413905240760474No Hit
CTCCAGCTCGGCTTTCGCGGCGCCGAGATGCTGTCCTGCCGCC12280.14116062078486685No Hit
GGATGTGAAAGTCTTCCAGAAGAAATTCTTGCAGCCAGCTTTG11950.13736721648038752No Hit
CGGCAGGACAGCATCTCGGCGCCGCGAAAGCCGAGCTGGAGAG11620.13357381217590822No Hit
GCCAGGGACTTCTGCAGAAACTGACGGAGTCTGGGGTCCGAGG11560.1328841023023665No Hit
GATCAGAGGTCTGATATGGACAATACTAGTTAAGAAAGCTAAC11250.12932060128906775No Hit
GATCTGCTAACTCAAACCCGGCTATGGCACCCCGAGAACGCAA11100.12759632660521353No Hit
ATGTGAAAGTCTTCCAGAAGAAATTCTTGCAGCCAGCTTTGCG11020.12667671344049125No Hit
CGCCTAGAGTTTGACCAGCCACTCTCCAGCTCGGCTTTCGCGG11020.12667671344049125No Hit
GTTCGGAGCCATCGCTGCTGCCTGCTGATCCGCGCCTAGAGTT10920.12552719698458842No Hit
ATGCTGTCCTGCCGCCTCCAGTGCGCGCTGGCTGCGCTGTCCA10660.1225384541992411No Hit
GTCCGAGGGAGCGCCGGTGACACAGCCCAGGGCCAGGACGATG10440.12000951799625488No Hit
GGACAGCATCTCGGCGCCGCGAAAGCCGAGCTGGAGAGTGGCT10420.1197796147050743No Hit
GTGTGGGGGCGAGGGATCAGAGGTCTGATATGGACAATACTAG10360.11908990483153262No Hit
CCCCAGACTCCGTCAGTTTCTGCAGAAGTCCCTGGCTGCTGCC10360.11908990483153262No Hit
GCAGAGATCTGCTAACTCAAACCCGGCTATGGCACCCCGAGAA10320.11863009824917149No Hit
GCTCAGCAGCCTGGGACAGATCTTCAGGTTCCAGGGCATCATT10300.11840019495799091No Hit
GAGATGGGGTGTGGGGGCGAGGGATCAGAGGTCTGATATGGAC10280.11817029166681035No Hit
GTATTTACAGTTTTCAGTTTCTAATGCAAGGGTCTCGCTGAAG10180.11702077521090752No Hit
GTACATGGGAAGCCGCTTTAGGAGCGAGGTTCGGAGCCATCGC10170.11690582356531724No Hit
GTACTTGGCCAGTTCCTGCTTCCCCGCGGCAGCAGCCAGGGAC10130.11644601698295612No Hit
GATCTTCAGGTTCCAGGGCATCATTCTCCGTCTGGTTGGGTTC10010.11506659723587273No Hit
AGTCTGGGGTCCGAGGGAGCGCCGGTGACACAGCCCAGGGCCA9960.11449183900792133No Hit
CTGCAGCTCAAGCCTCATTTCATCCTGCTCAGCAGCCTGGGAC9910.1139170807799699No Hit
GCCATAGCCGGGTTTGAGTTAGCAGATCTCTGCAGCTCAAGCC9850.11322737090642822No Hit
GGTCTGATATGGACAATACTAGTTAAGAAAGCTAACAGGATGT9420.10828445014604607No Hit
GTCCTGCCGCCTCCAGTGCGCGCTGGCTGCGCTGTCCATCGTC9060.10414619090479589No Hit
ACGCAAAGCTGGCTGCAAGAATTTCTTCTGGAAGACTTTCACA9040.10391628761361534No Hit
CTGCTGAGCAGGATGAAATGAGGCTTGAGCTGCAGAGATCTGC9030.10380133596802506No Hit
GCTCAAGCCTCATTTCATCCTGCTCAGCAGCCTGGGACAGATC9010.10357143267684449No Hit
CTGTTAGCTTTCTTAACTAGTATTGTCCATATCAGACCTCTGA8990.10334152938566393No Hit
GAGCTGCAGAGATCTGCTAACTCAAACCCGGCTATGGCACCCC8910.10242191622094166No Hit
GATCTCTGCAGCTCAAGCCTCATTTCATCCTGCTCAGCAGCCT8830.10150230305621939No Hit
GTCAAACTCTAGGCGCGGATCAGCAGGCAGCAGCGATGGCTCC8810.10127239976503884No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAATTA400.001930811623.12500215
ATCTCGT603.7249443E-521.58333437
TCTAATA1052.2575477E-819.3809532
CGTTATT1409.458745E-1118.52
CGCTAGA1409.458745E-1118.537
TACTATC609.234352E-418.57
GCGGTTT500.007033542718.49999835
GCGTTAT1552.0008883E-1117.9032251
CCGCTAG1351.1514203E-917.81481436
AAGACGG1705.456968E-1217.4117645
CAGTACT852.7218835E-517.4117644
CGTATTG1603.45608E-1117.3437527
CGCAAGA1603.45608E-1117.343752
CTACGCT650.001579516617.07692328
GTATAAT650.001579516617.0769231
CTATAAA904.4439566E-516.4444451
GCATTCG1606.2755134E-1016.187522
ATTCGTA1504.663889E-916.03333324
TTCGTAT1504.663889E-916.03333325
TCGTATT1504.663889E-916.03333326
Produced by FastQC (version 0.11.5)