FastQCFastQC Report
Fri 10 Feb 2017
ERR1630509.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630509.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences972648
Sequences flagged as poor quality0
Sequence length43
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTATAC502.7016882E-422.23
CTTACCC909.47457E-820.5555553
TTAACGG555.142312E-420.18181835
AGTGATA609.235426E-418.52
GTATACT609.235426E-418.54
GGTATCA5450.017.9908261
TAATACT1054.7953836E-717.6190474
GTCCTAA852.7224178E-517.4117641
CGCAAAT752.0668542E-417.26666627
CGCCTTA650.001579697617.07692325
CTATTAG650.001579697617.0769231
CTTATAC5000.016.6537
ATACCGC1901.8189894E-1216.55263127
TTAATAC1352.2178938E-816.4444453
GTCTTAC904.4448258E-516.4444451
TCTTACC1251.6568265E-716.2799992
AAGACGG2400.016.18755
GCCTAGA803.3818965E-416.18751
GCTTTAT803.3818965E-416.18751
TACCGCA1951.8189894E-1216.12820628