FastQCFastQC Report
Fri 10 Feb 2017
ERR1630508.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630508.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences519621
Sequences flagged as poor quality0
Sequence length43
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT187403.6064747190740944No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT151902.9232844707969847No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT131132.5235700635655602No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT80301.545357096807096No Hit
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA21340.41068394079531045No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT20550.395480552171679No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19530.3758508605310409No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15970.3073393877460688No Hit
GTACATGGGAATGGTATCAACGCAAAAAAAAAAAAAAAAAAAA15560.29944902149836133No Hit
CTTATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGT14270.27462323501167196No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12880.24787296895237104No Hit
GAATGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA11160.21477192030345196No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG10870.20919092954287838No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10340.198991187808037No Hit
TCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCTT10050.19341019704746343RNA PCR Primer, Index 27 (95% over 23bp)
GTACATGGGAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAA8870.17070133809064683No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTT8870.17070133809064683No Hit
TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8700.16742972281720717No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA8180.15742242903962697No Hit
GTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAA8010.15415081376618728No Hit
CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7550.14529820773217403No Hit
GTACATGGGAAGCAGAGGTATCAACGCAAAAAAAAAAAAAAAA7510.1445284159031294No Hit
GAACAGTGGTATCAACGCAGAAAAAAAAAAAAAAAAAAAAAAA6720.12932502727949793No Hit
ACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAAAAAAAAA6450.12412893243344669No Hit
CTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCGAGATTGAGC6340.12201200490357396No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATG6170.11874038963013427No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG6110.11758570188656733No Hit
ATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATG6030.11604611822847806No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC5980.11508387844217229No Hit
TGCCTACCCTACGCCGGCAGGCGCGGGTAACCCGTTGAACCCC5950.11450653457038881No Hit
GGTATCAACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAA5890.11335184682682185No Hit
TATCAACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAAAA5870.11296695091229952No Hit
ACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCC5590.10757840810898714No Hit
TCTTAGAGGGACAAGTGGCGTTCAGCCACCCGAGATTGAGCAA5430.1044992407928086No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGT5390.10372944896376397No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACTTCG250.00549360729.5999989
GCGTTAT405.9339603E-527.7500021
GCAGCGT405.9339603E-527.7500021
GTTATAC405.9339603E-527.7500023
GTCCTCA358.862827E-426.4285726
ATACTGG1150.025.739136
AATACTG951.0913936E-1125.315795
AGTCGGT2950.024.45762811
GGCAGTC3000.024.0500018
CCGTCTT1800.023.6388937
GCCGTCT1800.023.6388936
CTACACA551.9000587E-523.5454544
TGATGCT1103.6379788E-1223.54545413
GGGCAGT3400.023.3970587
CAGTCGG3100.023.27419310
ATGCTAG1054.0017767E-1122.90476415
TACTGGT1155.456968E-1222.521747
CGGTGAT3050.022.44262314
CTTATTG3000.022.228
GCAGTCG3250.022.1999999
Produced by FastQC (version 0.11.5)