##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR1630508.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 519621 Sequences flagged as poor quality 0 Sequence length 43 %GC 38 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.00301950844943 33.0 31.0 34.0 30.0 34.0 2 32.05923163228584 33.0 31.0 34.0 30.0 34.0 3 32.091395459382895 33.0 31.0 34.0 30.0 34.0 4 35.74900167622171 37.0 35.0 37.0 33.0 37.0 5 35.762690499421694 37.0 35.0 37.0 35.0 37.0 6 35.91166061417841 37.0 35.0 37.0 35.0 37.0 7 35.83032056056241 37.0 35.0 37.0 35.0 37.0 8 35.90344308640336 37.0 35.0 37.0 35.0 37.0 9 37.50044744150063 39.0 37.0 39.0 35.0 39.0 10 37.44202409063529 39.0 37.0 39.0 34.0 39.0 11 37.581102380388785 39.0 37.0 39.0 35.0 39.0 12 37.550035506648115 39.0 37.0 39.0 35.0 39.0 13 37.60178668683521 39.0 37.0 39.0 35.0 39.0 14 38.66865465406517 40.0 38.0 41.0 34.0 41.0 15 38.625346165763126 40.0 38.0 41.0 34.0 41.0 16 38.54203159610562 40.0 38.0 41.0 34.0 41.0 17 38.71748254978147 40.0 38.0 41.0 35.0 41.0 18 38.816487401394475 40.0 38.0 41.0 35.0 41.0 19 38.94354924069658 40.0 38.0 41.0 35.0 41.0 20 38.93816454685242 40.0 38.0 41.0 35.0 41.0 21 38.889021036486206 40.0 38.0 41.0 35.0 41.0 22 38.765284697885576 40.0 38.0 41.0 35.0 41.0 23 38.639741272966255 40.0 38.0 41.0 35.0 41.0 24 38.54709297738159 40.0 38.0 41.0 35.0 41.0 25 38.44599044303444 40.0 38.0 41.0 34.0 41.0 26 38.16189491956638 40.0 37.0 41.0 34.0 41.0 27 37.90268484145175 40.0 36.0 41.0 33.0 41.0 28 37.70083002803967 40.0 36.0 41.0 33.0 41.0 29 37.49520131018569 39.0 35.0 41.0 33.0 41.0 30 37.19127979816058 39.0 35.0 41.0 33.0 41.0 31 36.85864697539168 39.0 35.0 41.0 33.0 41.0 32 36.511136001046914 38.0 35.0 41.0 32.0 41.0 33 36.205318876642785 38.0 35.0 41.0 31.0 41.0 34 35.9541511986621 38.0 35.0 40.0 30.0 41.0 35 35.643505555010286 38.0 35.0 40.0 30.0 41.0 36 35.35067289428256 38.0 35.0 40.0 27.0 41.0 37 34.92578629424138 37.0 35.0 40.0 25.0 41.0 38 34.488504121273 37.0 35.0 40.0 22.0 41.0 39 34.112491604457865 37.0 34.0 40.0 20.0 41.0 40 33.58828646263334 36.0 33.0 40.0 15.0 41.0 41 33.16374241995609 35.0 33.0 40.0 12.0 41.0 42 32.60465031243926 35.0 33.0 40.0 10.0 41.0 43 31.726492963140444 35.0 31.0 40.0 8.0 41.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 8 1.0 9 5.0 10 12.0 11 8.0 12 8.0 13 10.0 14 4.0 15 5.0 16 5.0 17 7.0 18 25.0 19 60.0 20 141.0 21 236.0 22 481.0 23 905.0 24 1389.0 25 2259.0 26 3340.0 27 5236.0 28 7501.0 29 10167.0 30 12615.0 31 14933.0 32 18180.0 33 22317.0 34 28531.0 35 34802.0 36 48620.0 37 90173.0 38 100680.0 39 116965.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 50.83705239010741 18.616260697700824 13.887044595965136 16.659642316226634 2 17.40922710975884 25.18508682289592 34.348111411971416 23.05757465537382 3 22.627261022937873 30.570358010934896 29.551153629279803 17.251227336847432 4 12.07072077533433 16.588244124082745 30.668891365052602 40.67214373553032 5 10.580403794303924 45.639033064483534 29.78728727283924 13.993275868373296 6 29.690870846251403 32.17498907857843 19.221509523287164 18.912630551883005 7 24.662975514846398 35.431593411351734 21.84207335731235 18.063357716489516 8 34.55884192517239 28.2203760048189 19.357955124985327 17.862826945023393 9 29.571168216834963 10.562506134278637 22.935370202512985 36.93095544637342 10 20.118701900038683 32.190769811073835 26.862848114298693 20.827680174588785 11 29.006333462273464 26.289353201660443 19.211887125424106 25.492426210641987 12 21.442551398038184 33.86506703924591 27.427105524988406 17.2652760377275 13 30.864226041672683 20.387359248375258 29.68702188710618 19.06139282284588 14 23.30544762432619 23.595274247961495 31.677318661101072 21.42195946661124 15 26.73256084723289 26.533184763510327 29.605231505270186 17.129022883986597 16 19.864670596453955 26.145787025543616 31.968492420437205 22.021049957565225 17 18.658214352383755 29.796139878873255 31.263363105032322 20.282282663710667 18 17.17732732125915 23.702660208113222 36.77295567346201 22.347056797165628 19 18.111469705804808 27.202903654779153 38.3950995052163 16.290527134199735 20 19.487087704307562 24.1144988366521 39.67218414960134 16.726229309438995 21 21.342286012305124 25.4685626639416 36.79085333348729 16.398297990265984 22 21.05053490909721 25.984708085316026 35.472199930333844 17.492557075252925 23 18.71036774880153 26.657121247986517 36.43328502889606 18.199225974315898 24 16.75894546217339 29.12642098760443 36.28837171707841 17.82626183314377 25 16.899240023016777 28.05814237684774 36.92518200765558 18.117435592479904 26 17.858015746091862 28.8629597341139 36.518731921920015 16.760292597874223 27 17.26104218266775 29.64044948144898 36.222554515695094 16.875953820188176 28 16.026103640922905 29.528444770322988 37.99461530615583 16.450836282598278 29 16.52897015324631 30.0064854961597 37.7163355599562 15.748208790637792 30 14.929535180448827 31.167331574359004 37.932839511875 15.970293733317167 31 15.832116100003656 31.903252562925672 35.8241872441645 16.440444092906176 32 14.520005927397083 31.671737670340498 36.62573298615722 17.182523416105198 33 14.181105074660186 31.85494812565312 37.14515002280508 16.818796776881612 34 15.735699673415818 31.65384001031521 35.998545093443106 16.611915222825868 35 14.463618675919566 32.58143916431399 35.91617736773533 17.038764792031113 36 15.034034421241635 32.665538921637115 34.735509149938125 17.564917507183118 37 14.953783623063732 32.322981557712254 35.13156704598159 17.59166777324242 38 15.025181815207622 31.322252179954233 35.05708968652152 18.59547631831662 39 15.335023026398087 32.21001460679996 35.170633981305606 17.28432838549635 40 14.265974623812355 32.4307524137785 35.28032931694447 18.022943645464675 41 14.48247857573116 32.50388263753774 34.62273464698309 18.39090413974801 42 14.275597021675413 32.8048712426942 35.03168655616305 17.887845179467345 43 13.89301048264023 31.88516245494312 34.04616056702866 20.175666495387983 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 155.0 1 191.0 2 227.0 3 1099.5 4 1972.0 5 1972.0 6 3101.0 7 4230.0 8 4370.5 9 4511.0 10 6195.5 11 7880.0 12 7880.0 13 13979.0 14 20078.0 15 29830.0 16 39582.0 17 34499.5 18 29417.0 19 29417.0 20 30043.0 21 30669.0 22 21665.5 23 12662.0 24 11806.0 25 10950.0 26 10950.0 27 11364.0 28 11778.0 29 12096.0 30 12414.0 31 12846.5 32 13279.0 33 13279.0 34 14020.5 35 14762.0 36 15385.0 37 16008.0 38 18405.5 39 20803.0 40 20803.0 41 22594.0 42 24385.0 43 26123.5 44 27862.0 45 29538.0 46 31214.0 47 31214.0 48 32441.5 49 33669.0 50 31013.0 51 28357.0 52 27349.0 53 26341.0 54 26341.0 55 22808.5 56 19276.0 57 18107.0 58 16938.0 59 15884.0 60 14830.0 61 14830.0 62 13496.5 63 12163.0 64 11198.0 65 10233.0 66 9558.5 67 8884.0 68 8884.0 69 7182.5 70 5481.0 71 4655.0 72 3829.0 73 2941.0 74 2053.0 75 2053.0 76 1597.0 77 1141.0 78 842.0 79 543.0 80 458.0 81 373.0 82 373.0 83 322.0 84 271.0 85 245.0 86 219.0 87 163.0 88 107.0 89 107.0 90 82.5 91 58.0 92 37.0 93 16.0 94 8.5 95 1.0 96 1.0 97 0.5 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 43 519621.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 33.80350315026076 #Duplication Level Percentage of deduplicated Percentage of total 1 78.7158024980579 26.608698777184042 2 9.767684470725403 6.603639055538385 3 3.4761729834781603 3.5252047319356605 4 1.8081241761994753 2.444837251448864 5 1.073474876086264 1.8143605677753902 6 0.7856574070296902 1.5934783580132301 7 0.5401489571456919 1.278124888213912 8 0.44441835165882193 1.2018317720266145 9 0.35764584619907647 1.0880714239801343 >10 2.508403037775898 16.651066847568043 >50 0.2820752933857278 6.54469143635989 >100 0.21983600800304695 14.161602795714417 >500 0.01199105498198438 2.7173199056558976 >1k 0.006281028800087057 3.136942968228417 >5k 5.710026181897323E-4 1.549941670863536 >10k+ 0.001713007854569197 9.080187549493566 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT 18740 3.6064747190740944 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT 15190 2.9232844707969847 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT 13113 2.5235700635655602 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8030 1.545357096807096 No Hit GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA 2134 0.41068394079531045 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2055 0.395480552171679 No Hit GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1953 0.3758508605310409 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1597 0.3073393877460688 No Hit GTACATGGGAATGGTATCAACGCAAAAAAAAAAAAAAAAAAAA 1556 0.29944902149836133 No Hit CTTATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGT 1427 0.27462323501167196 No Hit AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1288 0.24787296895237104 No Hit GAATGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1116 0.21477192030345196 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG 1087 0.20919092954287838 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1034 0.198991187808037 No Hit TCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCTT 1005 0.19341019704746343 RNA PCR Primer, Index 27 (95% over 23bp) GTACATGGGAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAA 887 0.17070133809064683 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTT 887 0.17070133809064683 No Hit TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 870 0.16742972281720717 No Hit GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA 818 0.15742242903962697 No Hit GTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAA 801 0.15415081376618728 No Hit CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 755 0.14529820773217403 No Hit GTACATGGGAAGCAGAGGTATCAACGCAAAAAAAAAAAAAAAA 751 0.1445284159031294 No Hit GAACAGTGGTATCAACGCAGAAAAAAAAAAAAAAAAAAAAAAA 672 0.12932502727949793 No Hit ACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAAAAAAAAA 645 0.12412893243344669 No Hit CTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCGAGATTGAGC 634 0.12201200490357396 No Hit GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATG 617 0.11874038963013427 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG 611 0.11758570188656733 No Hit ATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATG 603 0.11604611822847806 No Hit GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC 598 0.11508387844217229 No Hit TGCCTACCCTACGCCGGCAGGCGCGGGTAACCCGTTGAACCCC 595 0.11450653457038881 No Hit GGTATCAACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAA 589 0.11335184682682185 No Hit TATCAACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAAAA 587 0.11296695091229952 No Hit ACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCC 559 0.10757840810898714 No Hit TCTTAGAGGGACAAGTGGCGTTCAGCCACCCGAGATTGAGCAA 543 0.1044992407928086 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGT 539 0.10372944896376397 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 1.9244795726115765E-4 0.0 2 0.0 0.0 0.0 0.0017320316153504188 0.0 3 0.0 0.0 0.0 0.004233855059745468 0.0 4 0.0 0.0 0.0 0.006735678504140518 0.0 5 0.0 0.0 0.0 0.007890366247707463 0.0 6 0.0 0.0 0.0 0.007890366247707463 0.0 7 0.0 0.0 0.0 0.008660158076752094 0.0 8 0.0 0.0 0.0 0.008660158076752094 0.0 9 0.0 0.0 0.0 0.014433596794586823 0.0 10 0.0 0.0 0.0 0.02367109874312239 0.0 11 0.0 0.0 0.0 0.029252089503695962 0.0 12 0.0 0.0 0.0 0.036950007794142266 0.0 13 0.0 0.0 0.0 0.04118386285388774 0.0 14 0.0 0.0 0.0 0.046379957699938995 0.0 15 0.0 0.0 0.0 0.0554250116912134 0.0 16 0.0 0.0 0.0 0.07967345430611927 0.0 17 0.0 0.0 0.0 0.11027267951064333 0.0 18 0.0 0.0 0.0 0.12432138039070784 0.0 19 0.0 0.0 0.0 0.14703023934752443 0.0 20 0.0 0.0 0.0 0.16069404431306664 0.0 21 0.0 0.0 0.0 0.20418728265408828 0.0 22 0.0 0.0 0.0 0.2871323522336472 0.0 23 0.0 0.0 0.0 0.4291589446923816 0.0 24 0.0 0.0 0.0 0.6368102905771706 0.0 25 0.0 0.0 0.0 0.6754923299866633 0.0 26 0.0 0.0 0.0 0.7420793231990239 0.0 27 0.0 0.0 0.0 0.7915384482151414 0.0 28 0.0 0.0 0.0 0.8635139842308144 0.0 29 0.0 0.0 0.0 0.9726319759978908 0.0 30 0.0 0.0 0.0 1.2051091083693692 0.0 31 0.0 0.0 0.0 1.8143993410581944 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CACTTCG 25 0.005493607 29.599998 9 GCGTTAT 40 5.9339603E-5 27.750002 1 GCAGCGT 40 5.9339603E-5 27.750002 1 GTTATAC 40 5.9339603E-5 27.750002 3 GTCCTCA 35 8.862827E-4 26.428572 6 ATACTGG 115 0.0 25.73913 6 AATACTG 95 1.0913936E-11 25.31579 5 AGTCGGT 295 0.0 24.457628 11 GGCAGTC 300 0.0 24.050001 8 CCGTCTT 180 0.0 23.63889 37 GCCGTCT 180 0.0 23.63889 36 CTACACA 55 1.9000587E-5 23.545454 4 TGATGCT 110 3.6379788E-12 23.545454 13 GGGCAGT 340 0.0 23.397058 7 CAGTCGG 310 0.0 23.274193 10 ATGCTAG 105 4.0017767E-11 22.904764 15 TACTGGT 115 5.456968E-12 22.52174 7 CGGTGAT 305 0.0 22.442623 14 CTTATTG 300 0.0 22.2 28 GCAGTCG 325 0.0 22.199999 9 >>END_MODULE