FastQCFastQC Report
Fri 10 Feb 2017
ERR1630507.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630507.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1487196
Sequences flagged as poor quality0
Sequence length43
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT42980.2890002393766524No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT39100.2629108738861589No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT39010.2623057081917918No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24730.16628608468554246No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTAACGG1059.840733E-1021.14285735
ACCGTTA450.003826330220.5555538
TAACGGC1101.7553248E-920.18181836
CTAGTAC1052.260822E-819.380953
TACCGTC1552.1827873E-1117.9032277
ATACCGT1603.45608E-1117.343756
GACGGTA1851.8189894E-1217.08
TAGTACT1757.2759576E-1216.9142864
TACGACG1901.8189894E-1216.552635
GGTATCA25150.016.2564621
ACCGTCG1606.2937033E-1016.18758
GTATTGA1902.7284841E-1115.5789461
CGTCGTA1557.212293E-915.516129510
CCGTCGT1601.0970325E-815.031259
TCTGTCG750.004105160514.88
TATTAGA2750.014.82
GTATTAG4000.014.7999991
TACGCTA1001.0937073E-414.7999999
ACGACGG2151.2732926E-1114.6279076
GTAAACG2550.014.50980427
Produced by FastQC (version 0.11.5)