FastQCFastQC Report
Fri 10 Feb 2017
ERR1630506.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630506.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences877474
Sequences flagged as poor quality0
Sequence length43
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT32710.3727745779362124No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT30080.34280217989364925No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT24100.2746520124812815No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13500.1538507123857801No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAACGTA250.005495642329.623
GCATTAG502.7013954E-422.21
CGAACGA1251.8189894E-1120.7216
GTCTATA450.003825097820.5555551
ACGAACG1403.6379788E-1219.82142815
TAACGAA1457.2759576E-1219.1379313
GTACTGA1103.848436E-818.5000026
ATAACGA1409.458745E-1118.512
CTCTATG3450.018.2318841
TCTATGG3350.017.6716422
GAGTACG1800.017.4722211
GACTGCG650.001579531717.0769237
CGAAGCG1352.2169843E-816.44444528
AACGAGA1708.54925E-1116.3235318
TACGGGA1251.6562808E-716.2799992
TATGGGC3650.016.2191774
CTAGACA1059.33798E-615.8571424
GACCATA1403.4693585E-815.85714211
ATAAACG1403.4693585E-815.85714215
CGATCGC1059.33798E-615.85714219
Produced by FastQC (version 0.11.5)