##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1630506.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	877474
Sequences flagged as poor quality	0
Sequence length	43
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.08570738278285	33.0	31.0	34.0	30.0	34.0
2	32.25903217645195	34.0	31.0	34.0	30.0	34.0
3	32.36211785192496	34.0	31.0	34.0	30.0	34.0
4	35.93798562692456	37.0	35.0	37.0	35.0	37.0
5	35.89036598235389	37.0	35.0	37.0	35.0	37.0
6	35.964606358706924	37.0	35.0	37.0	35.0	37.0
7	35.918514964545956	37.0	35.0	37.0	35.0	37.0
8	35.91560775589932	37.0	35.0	37.0	35.0	37.0
9	37.638467920417014	39.0	37.0	39.0	35.0	39.0
10	37.54870913554134	39.0	37.0	39.0	35.0	39.0
11	37.64425726574235	39.0	37.0	39.0	35.0	39.0
12	37.586126768428464	39.0	37.0	39.0	35.0	39.0
13	37.61843769729929	39.0	37.0	39.0	35.0	39.0
14	38.92128997554344	40.0	38.0	41.0	36.0	41.0
15	38.93275926124307	40.0	38.0	41.0	36.0	41.0
16	38.893435019157266	40.0	38.0	41.0	35.0	41.0
17	38.873326161230985	40.0	38.0	41.0	35.0	41.0
18	38.86115144152419	40.0	38.0	41.0	35.0	41.0
19	38.92443764715536	40.0	38.0	41.0	35.0	41.0
20	38.900212427946585	40.0	38.0	41.0	35.0	41.0
21	38.84949639533479	40.0	38.0	41.0	35.0	41.0
22	38.788140731235345	40.0	38.0	41.0	35.0	41.0
23	38.72943585792856	40.0	38.0	41.0	35.0	41.0
24	38.67564509033886	40.0	38.0	41.0	34.0	41.0
25	38.62845052958834	40.0	38.0	41.0	34.0	41.0
26	38.491359288138455	40.0	38.0	41.0	34.0	41.0
27	38.36956536603934	40.0	38.0	41.0	34.0	41.0
28	38.27450385994343	40.0	38.0	41.0	34.0	41.0
29	38.20124015070532	40.0	38.0	41.0	34.0	41.0
30	38.11491394616821	40.0	38.0	41.0	33.0	41.0
31	38.074168579353916	40.0	37.0	41.0	33.0	41.0
32	37.97230003396112	40.0	37.0	41.0	33.0	41.0
33	37.89431139840041	40.0	37.0	41.0	33.0	41.0
34	37.83275857746212	40.0	37.0	41.0	33.0	41.0
35	37.769655853050914	40.0	37.0	41.0	33.0	41.0
36	37.73040568723404	40.0	37.0	41.0	33.0	41.0
37	37.602346052418646	40.0	37.0	41.0	33.0	41.0
38	37.455921201084024	40.0	37.0	41.0	32.0	41.0
39	37.40559720288009	40.0	36.0	41.0	32.0	41.0
40	37.23787257514183	40.0	36.0	41.0	31.0	41.0
41	37.180224143393424	40.0	36.0	41.0	31.0	41.0
42	37.051150233511194	40.0	36.0	41.0	31.0	41.0
43	36.23021650783955	39.0	35.0	40.0	30.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	3.0
11	2.0
12	0.0
13	3.0
14	2.0
15	3.0
16	3.0
17	8.0
18	19.0
19	45.0
20	86.0
21	183.0
22	378.0
23	682.0
24	1140.0
25	1867.0
26	2855.0
27	4194.0
28	6147.0
29	8825.0
30	11845.0
31	15388.0
32	20040.0
33	26029.0
34	34746.0
35	47224.0
36	67284.0
37	104097.0
38	192833.0
39	331542.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.92038966396725	18.44407925476994	11.977448904468964	25.658082176793844
2	19.151336677781906	20.701696004667944	34.26369328321979	25.883274034330363
3	20.575880310983575	22.684546778594008	29.194483255344316	27.545089655078097
4	14.640091900158867	15.27304512726303	33.53444090651119	36.55242206606692
5	14.695820047089715	36.65692658699859	33.92693116833092	14.720322197580781
6	35.81667377039091	33.822312683908585	15.403305397082988	14.95770814861751
7	29.643499408529483	30.013652826180603	20.853723301203225	19.489124464086686
8	27.727773130599882	32.796641268003384	19.7356274943759	19.739958107020836
9	26.98154019378352	13.297260089757646	19.27521499212512	40.44598472433371
10	17.10933885220531	26.641587101156272	32.57908496434083	23.669989082297597
11	36.113206773078176	21.23322172508815	20.952529647602095	21.70104185423158
12	22.07803308132207	25.24222939938961	28.537027877749082	24.142709641539238
13	30.706322922388583	19.28364829043368	24.893956971944466	25.11607181523327
14	23.517961785762314	20.580894704572444	24.86444042786453	31.036703081800717
15	26.434059584671456	26.969118173302004	22.115641033238592	24.48118120878795
16	25.778769513398686	25.857176394970104	23.680587686928618	24.68346640470259
17	24.001622840106943	26.162826476909856	25.17271167008937	24.662839012893826
18	23.830107786669462	24.51548421947545	26.82164941639296	24.832758577462126
19	24.509330190980016	25.053619822353713	26.53856410560313	23.89848588106314
20	25.238582567688617	24.07980179469705	26.397249377189524	24.28436626042481
21	25.47015638070188	24.546254361952606	25.54787948132936	24.435709776016157
22	25.34616410286801	24.560385834793966	25.523035440366325	24.570414621971707
23	24.604261778696575	24.843471145583802	25.729195394963266	24.823071680756353
24	24.621128375313685	25.272771614885453	25.77569249915097	24.330407510649888
25	24.927006384234744	24.67377950799682	25.705604952397447	24.693609155370986
26	24.614860383327596	25.23938031212321	25.754039435926305	24.39171986862289
27	25.05749458103602	24.622040083238932	25.480299131370277	24.840166204354773
28	24.116725965669637	24.889740322790193	26.24681756952343	24.746716142016744
29	23.71249746431233	25.291461627353062	26.469046376302884	24.52699453203172
30	23.846176638851976	25.89683568971844	26.292744856257848	23.964242815171733
31	24.567793461686612	25.51255079922596	25.495570239118198	24.42408549996923
32	23.52331806982315	25.41146518301397	26.132170297923356	24.93304644923952
33	23.834438399314394	25.02148211798868	26.373658934623705	24.77042054807322
34	24.46420064868019	24.790820012900667	26.08726868260484	24.657710655814302
35	24.212797188292758	25.00940198797913	26.641473137665617	24.136327686062494
36	23.569245356557573	25.424457020948772	26.26527965500972	24.741017967483934
37	24.281175282686437	24.642097657594412	26.358615753857094	24.718111305862053
38	23.604004221207695	24.591611831233745	27.144394021931134	24.659989925627425
39	23.736543760840778	24.485739748414197	26.968206465376753	24.809510025368272
40	23.151911053774814	24.400267130422097	27.813587639063947	24.634234176739138
41	22.658791029705725	24.61862117851925	28.081515805596517	24.64107198617851
42	23.23009000836492	24.241629951428763	28.005046303366253	24.523233736840066
43	22.489327319100052	24.00743497813041	28.158669088770722	25.34456861399882
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	16.0
1	29.0
2	42.0
3	217.5
4	393.0
5	393.0
6	603.0
7	813.0
8	836.5
9	860.0
10	1144.5
11	1429.0
12	1429.0
13	2419.0
14	3409.0
15	5293.0
16	7177.0
17	6742.0
18	6307.0
19	6307.0
20	7263.5
21	8220.0
22	7691.5
23	7163.0
24	8364.0
25	9565.0
26	9565.0
27	11429.5
28	13294.0
29	16037.5
30	18781.0
31	21369.0
32	23957.0
33	23957.0
34	27013.0
35	30069.0
36	33025.0
37	35981.0
38	40081.5
39	44182.0
40	44182.0
41	46659.5
42	49137.0
43	49640.0
44	50143.0
45	52730.0
46	55317.0
47	55317.0
48	57502.5
49	59688.0
50	60806.5
51	61925.0
52	64938.5
53	67952.0
54	67952.0
55	64410.5
56	60869.0
57	59159.0
58	57449.0
59	53349.0
60	49249.0
61	49249.0
62	45916.0
63	42583.0
64	37939.5
65	33296.0
66	29258.5
67	25221.0
68	25221.0
69	21867.0
70	18513.0
71	16065.5
72	13618.0
73	10969.0
74	8320.0
75	8320.0
76	6627.5
77	4935.0
78	4157.5
79	3380.0
80	2685.0
81	1990.0
82	1990.0
83	1511.5
84	1033.0
85	805.0
86	577.0
87	480.0
88	383.0
89	383.0
90	295.5
91	208.0
92	115.0
93	22.0
94	13.0
95	4.0
96	4.0
97	4.0
98	4.0
99	2.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	877474.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	67.12794705482652
#Duplication Level	Percentage of deduplicated	Percentage of total
1	88.75907464035436	59.58214463093103
2	7.114969689727513	9.552266172574479
3	1.692438978867651	3.4082986250085705
4	0.6581128026227305	1.7671104548224859
5	0.36079077064993537	1.210957187502946
6	0.2386175339731335	0.9610743112141064
7	0.16090149561221317	0.7560690954949334
8	0.11295904495567022	0.6066167031318399
9	0.09389305947026733	0.5672563492442205
>10	0.6489110508895416	8.859450247307514
>50	0.10378082893672104	4.924811523396609
>100	0.054186911221593276	6.40089325026146
>500	6.815963594173276E-4	0.25473630989463053
>1k	6.815963594173276E-4	1.1483151392151754
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	3271	0.3727745779362124	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	3008	0.34280217989364925	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	2410	0.2746520124812815	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1350	0.1538507123857801	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	1.139634906561334E-4	0.0	0.0	0.0015954888691858675	0.0
3	1.139634906561334E-4	0.0	0.0	0.0026211602850910684	0.0
4	1.139634906561334E-4	0.0	0.0	0.004672503116901469	0.0
5	1.139634906561334E-4	0.0	0.0	0.0049004300982137365	0.0
6	1.139634906561334E-4	0.0	0.0	0.005014393588869869	0.0
7	1.139634906561334E-4	0.0	0.0	0.005470247551494403	0.0
8	1.139634906561334E-4	0.0	0.0	0.00569817453280667	0.0
9	1.139634906561334E-4	0.0	0.0	0.009800860196427473	0.0
10	1.139634906561334E-4	0.0	0.0	0.014359399822672808	0.0
11	1.139634906561334E-4	0.0	0.0	0.017322450579732276	0.0
12	1.139634906561334E-4	0.0	0.0	0.018803975958262012	0.0
13	1.139634906561334E-4	0.0	0.0	0.019259829920886543	0.0
14	2.279269813122668E-4	0.0	0.0	0.02085531879007241	0.0
15	2.279269813122668E-4	0.0	0.0	0.022564771149914415	0.0
16	2.279269813122668E-4	0.0	0.0	0.025983675869598415	0.0
17	2.279269813122668E-4	0.0	0.0	0.02940258058928242	0.0
18	2.279269813122668E-4	1.139634906561334E-4	0.0	0.030656178986499884	0.0
19	2.279269813122668E-4	1.139634906561334E-4	0.0	0.03384715672487162	0.0
20	2.279269813122668E-4	1.139634906561334E-4	0.0	0.03624039002865042	0.0
21	2.279269813122668E-4	1.139634906561334E-4	0.0	0.04022911220161509	0.0
22	2.279269813122668E-4	1.139634906561334E-4	0.0	0.04683899465967083	0.0
23	2.279269813122668E-4	1.139634906561334E-4	0.0	0.05812138023462803	0.0
24	2.279269813122668E-4	1.139634906561334E-4	0.0	0.07384834194517445	0.0
25	2.279269813122668E-4	1.139634906561334E-4	0.0	0.07954651647798111	0.0
26	2.279269813122668E-4	1.139634906561334E-4	0.0	0.08706810686128592	0.0
27	2.279269813122668E-4	1.139634906561334E-4	0.0	0.0985784194175554	0.0
28	2.279269813122668E-4	1.139634906561334E-4	0.0	0.12376435085256088	0.0
29	2.279269813122668E-4	1.139634906561334E-4	0.0	0.16342364560089528	0.0
30	2.279269813122668E-4	1.139634906561334E-4	0.0	0.22325447819536534	0.0
31	2.279269813122668E-4	1.139634906561334E-4	0.0	0.4463949929000745	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AAACGTA	25	0.0054956423	29.6	23
GCATTAG	50	2.7013954E-4	22.2	1
CGAACGA	125	1.8189894E-11	20.72	16
GTCTATA	45	0.0038250978	20.555555	1
ACGAACG	140	3.6379788E-12	19.821428	15
TAACGAA	145	7.2759576E-12	19.13793	13
GTACTGA	110	3.848436E-8	18.500002	6
ATAACGA	140	9.458745E-11	18.5	12
CTCTATG	345	0.0	18.231884	1
TCTATGG	335	0.0	17.671642	2
GAGTACG	180	0.0	17.472221	1
GACTGCG	65	0.0015795317	17.076923	7
CGAAGCG	135	2.2169843E-8	16.444445	28
AACGAGA	170	8.54925E-11	16.32353	18
TACGGGA	125	1.6562808E-7	16.279999	2
TATGGGC	365	0.0	16.219177	4
CTAGACA	105	9.33798E-6	15.857142	4
GACCATA	140	3.4693585E-8	15.857142	11
ATAAACG	140	3.4693585E-8	15.857142	15
CGATCGC	105	9.33798E-6	15.857142	19
>>END_MODULE