FastQCFastQC Report
Fri 10 Feb 2017
ERR1630505.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630505.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1510397
Sequences flagged as poor quality0
Sequence length43
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCAGGACAGGCTGCATCAGAAGAGGCCATCAAGCAGATCAC93450.6187115043263459No Hit
GTGCAGGAGGCGGCGGGTGGGGGGCTGCCTGCGGGCTGCGTCT55950.3704324094923388No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT53920.3569922344919912No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT51790.3428899819054196No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT44370.29376382500759735No Hit
GGTCTTGGGTGTGTAGAAGAAGCCTCGTTCCCCGCACACTAGG38530.2550984939721146No Hit
AGGTAGAGAGCTTCCACCAGGTGTGAGCCGCACAGGTGTTGGT33560.22219323793678086No Hit
GCCCTGGAGGGGTCCCTGCAGAAGCGTGGCATTGTGGAACAAT32760.2168966172469887No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32540.21544004655729584No Hit
CCCAAGACCCGCCGGGAGGCAGAGGACCTGCAGGTGGGGCAGG31340.2074951155226076No Hit
CTTCTACACACCCAAGACCCGCCGGGAGGCAGAGGACCTGCAG30920.20471438966046676No Hit
CTCCAGGGCCAAGGGCTGCAGGCTGCCTGCACCAGGGCCCCCG28600.1893541896600695No Hit
CTTCTGCAGGGACCCCTCCAGGGCCAAGGGCTGCAGGCTGCCT27970.18518310086685819No Hit
CATCAGAAGAGGCCATCAAGCAGATCACTGTCCTTCTGCCATG27910.18478585431512376No Hit
GGTGTGAGCCGCACAGGTGTTGGTTCACAAAGGCTGCGGCTGG27050.1790919870735972No Hit
ATGCTGTACCAGCATCTGCTCCCTCTACCAGCTGGAGAACTAC25820.17094843276304175No Hit
ACCAACACCTGTGCGGCTCACACCTGGTGGAAGCTCTCTACCT25520.1689622000043697No Hit
CTGCAGAAGCGTGGCATTGTGGAACAATGCTGTACCAGCATCT25370.16796908362503368No Hit
GTGTAGAAGAAGCCTCGTTCCCCGCACACTAGGTAGAGAGCTT24900.1648573189697808No Hit
GGTGTTGGTTCACAAAGGCTGCGGCTGGGTCAGGTCCCCAGAG23130.15313854569361565No Hit
GCTTTATTCCATCTCTCTCGGTGCAGGAGGCGGCGGGTGGGGG22930.15181439052116763No Hit
GTACAGCATTGTTCCACAATGCCACGCTTCTGCAGGGACCCCT22220.14711363965897709No Hit
CTGGGGACCTGACCCAGCCGCAGCCTTTGTGAACCAACACCTG21410.14175081121056252No Hit
TCTCTACCTAGTGTGCGGGGAACGAGGCTTCTTCTACACACCC20890.13830800776219762No Hit
GCTCACACCTGGTGGAAGCTCTCTACCTAGTGTGCGGGGAACG20060.13281276379653828No Hit
TGCTGGTACAGCATTGTTCCACAATGCCACGCTTCTGCAGGGA19930.13195206293444706No Hit
GGTCCCCAGAGGGCCAGCAGCGCCAGCAGGGGCAGGAGGCGCA18200.12049812069277151No Hit
CTCTACCTAGTGTGCGGGGAACGAGGCTTCTTCTACACACCCA16790.11116282672701283No Hit
CACCTGGTGGAAGCTCTCTACCTAGTGTGCGGGGAACGAGGCT16480.10911038620971837No Hit
GCCGCAGCCTTTGTGAACCAACACCTGTGCGGCTCACACCTGG15940.10553516724410866No Hit
CTAGGTAGAGAGCTTCCACCAGGTGTGAGCCGCACAGGTGTTG15910.10533654396824148No Hit
TTGCAGTAGTTCTCCAGCTGGTAGAGGGAGCAGATGCTGGTAC15900.10527033620961906No Hit
GAACAATGCTGTACCAGCATCTGCTCCCTCTACCAGCTGGAGA15820.10474067414063984No Hit
GCCCTGTGGATGCGCCTCCTGCCCCTGCTGGCGCTGCTGGCCC15690.10387997327854862No Hit
CCACAGGGCCATGGCAGAAGGACAGTGATCTGCTTGATGGCCT15330.10149649396814216No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGCTTA604.3246473E-827.74999825
CGCTTAT751.3744284E-824.66666626
TGTACCG652.6837733E-622.769235
AGCGTCA753.7423706E-722.23
GCTATAC656.903967E-519.9230773
GTACCGG801.6172185E-518.56
TACGAGT609.238649E-418.54
CGGTGAT2100.018.514
CCAGGAC23200.018.1810343
AAGACGG2500.017.765
CAGGACA24550.017.7087574
GACGGAC2200.017.659097
TGCATCA22650.017.47902914
TCGGTGA2350.017.31914913
TCCAGGA24650.017.1866132
TAGGACG650.001580244917.0769234
ATTGACG1851.8189894E-1217.032
GACAGGC23850.016.9098537
ACAGGCT23750.016.9031588
AGTCGGT2300.016.89130411
Produced by FastQC (version 0.11.5)