FastQCFastQC Report
Fri 10 Feb 2017
ERR1630504.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630504.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1269808
Sequences flagged as poor quality0
Sequence length43
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT36380.2865000063001651No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT32320.2545266685987173No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT24940.19640764588032206No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14690.11568678099366204No Hit
ACCTAGGCCTGGTCAGCATGTTGATGTATCTACGGAGATCAGC13190.10387397149805326No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGACCG250.005496557329.65
GCCGTCT2150.020.65116336
TAATACT2250.019.7333344
TGCCGTC2350.019.68085135
AAGACGG2300.019.3043485
GACGGAC2250.018.9111127
CTAAACG1205.175025E-918.526
AACGCAT1306.9667294E-1018.516
GTTAACG1258.580173E-917.7613
ACGGACC2500.017.768
GGTATCA16650.017.6666661
ATAGGAC2950.017.5593243
CAAGACG2650.017.4528314
ATGCCGT2550.017.41176434
ACGGAAT3550.017.19718418
TAACGCA1401.869921E-917.17857215
CGGACCA2600.017.0769239
AATCGAG3800.017.03947422
TCTATAC1201.041426E-716.9583343
CTATACT2850.016.8771934
Produced by FastQC (version 0.11.5)