FastQCFastQC Report
Fri 10 Feb 2017
ERR1630503.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630503.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1075682
Sequences flagged as poor quality0
Sequence length43
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT67310.6257425521669043No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT66400.6172828029101537No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT47390.4405577112938582No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT38650.35930693271803377No Hit
ACCTAGGCCTGGTCAGCATGTTGATGTATCTACGGAGATCAGC26890.24998094232310292No Hit
GGCCTAGGTATGGGAAAAGACACAAAGAGGACACGCTGGCCTT20940.19466719718281053No Hit
GTGTGGGAGCAGGGAGCAAGCTTTGGCCAGAGCCAAGGGTGCA18070.16798644952690478No Hit
GTATGCAGCTGATCTCCGTAGATACATCAACATGCTGACCAGG16970.1577603789967667No Hit
GGGTACACTGGCTCCAGTGGGGCTCCCTGGGCACCCAGCAGTG15910.14790616557681543No Hit
GCTTTATTGAGCCTGTGTGGGAGCAGGGAGCAAGCTTTGGCCA15830.14716245135644176No Hit
CCTCTGCCTCTCCCTGCTGCTCCTGTCCACCTGCGTGGCTCTG14460.13442634533254252No Hit
GCTGCATACTGGGCCATCTGCTCTGGTGTGGCATTGTCCCCTG13970.12987109573275374No Hit
AGTGTACCCAGGGGACAATGCCACACCAGAGCAGATGGCCCAG13810.12838366729200637No Hit
GTCTAGTGCCCATTTACTCTGGACTCCGGATGGCTGCCGCACG13210.12280581063920377No Hit
CTGTTACTACAGCCACTGCTGGGTGCCCAGGGAGCCCCACTGG12350.11481088277018672No Hit
GCTCATGGAGTCGTAGGAGACAGAAGGTGGCATTATAAGTCCA11160.10374813374212825No Hit
GTGCAGAGGGGAGAGCTGGGCTGGCGCTGCTCATGGAGTCGTA11100.103190348076848No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11040.10263256241156773No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT609.236291E-418.529
TTAACGG609.236291E-418.535
GCTTTAT4650.018.3010771
GAACCGC852.7228518E-517.4117646
CTATATC752.0671105E-417.2666664
TCGTATG752.0671105E-417.26666630
CGTATGC650.001579844417.07692531
TAACGGC650.001579844417.07692536
AAGACGG1655.4569682E-1116.8181825
AACCGCG803.3823133E-416.18757
TTTCGGA1606.2937033E-1016.187530
GACGGAC1606.2937033E-1016.18757
GATAGTC1059.341902E-615.8571447
TATTGAG5300.015.7075465
TAGAACC1302.5883128E-715.6538484
AACGTGG957.059E-515.57894810
CTAATCA957.059E-515.5789484
TTTATTG5750.015.4434793
CAGTCGG2201.8189894E-1215.13636410
GACTTAA1601.0959411E-815.031251
Produced by FastQC (version 0.11.5)