FastQCFastQC Report
Fri 10 Feb 2017
ERR1630502.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630502.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences670124
Sequences flagged as poor quality0
Sequence length43
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT57540.8586470563656875No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT53690.8011950027159151No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT41280.6160053960162597No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25310.37769129295473675No Hit
GGATTACTCCGGTCTGAACTCAGATCACGTAGGACTTTAATCG8690.12967749252377173No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT7520.112218037258776No Hit
GAGTAATCCAGGTCGGTTTCTATCTACTTCAAATTCCTCCCTG7490.1117703589186479No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACTCCG250.00549472829.65
GTAGACG250.00549472829.64
CCGAGTT555.1400176E-420.18181813
AAGCCCG609.231339E-418.55
GTCTTAT500.00703189618.51
ATTCGTA1156.39302E-817.69565224
ACGCTTC1156.39302E-817.69565231
CGAACTA1800.017.47222124
ATGCCGT852.7203712E-517.41176634
TCGGTGA2150.017.20930313
AGTCGGT2150.017.20930311
GCCGAGT650.001579004717.07692312
GTGTAGT650.001579004717.0769231
CGTATTG1201.0392614E-716.95833227
GCATTCG1201.0392614E-716.95833222
CAGTCGG2200.016.81818210
CATAGTA1352.2142558E-816.4444453
AGTTCGC1801.0913936E-1116.4444458
TTCGTAT1251.654571E-716.27999925
TCGTATT1251.654571E-716.27999926
Produced by FastQC (version 0.11.5)