FastQCFastQC Report
Fri 10 Feb 2017
ERR1630501.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630501.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences257832
Sequences flagged as poor quality0
Sequence length43
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTGGGAGCAGGGAGCAAGCTTTGGCCAGAGCCAAGGGTGCA6870.26645257376896586No Hit
ACCTAGGCCTGGTCAGCATGTTGATGTATCTACGGAGATCAGC6760.26218622979304357No Hit
GCTTTATTGAGCCTGTGTGGGAGCAGGGAGCAAGCTTTGGCCA5690.2206863383908902No Hit
CTTATACACATCTCCGAGCCCACGAGACTCGACGTCATCTCGT5000.19392472617828663No Hit
AGTGTACCCAGGGGACAATGCCACACCAGAGCAGATGGCCCAG4460.17298085575103167No Hit
GGCCTAGGTATGGGAAAAGACACAAAGAGGACACGCTGGCCTT4460.17298085575103167No Hit
GCTGCATACTGGGCCATCTGCTCTGGTGTGGCATTGTCCCCTG4200.16289676998976077No Hit
CCTCTGCACCCTTGGCTCTGGCCAAAGCTTGCTCCCTGCTCCC4040.1566911787520556No Hit
GTATGCAGCTGATCTCCGTAGATACATCAACATGCTGACCAGG3980.15436408203791618No Hit
GTGCAGAGGGGAGAGCTGGGCTGGCGCTGCTCATGGAGTCGTA3870.15009773806199384No Hit
TCTCCGAGCCCACGAGACTCGACGTCATCTCGTATGCCGTCTT3840.14893418970492414No Hit
CCTCTGCCTCTCCCTGCTGCTCCTGTCCACCTGCGTGGCTCTG3790.14699494244314126No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA3720.14427999627664526No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC3260.12643892146824287No Hit
CCCTTGGCTCTGGCCAAAGCTTGCTCCCTGCTCCCACACAGGC3240.12566322256352974No Hit
GGGTACACTGGCTCCAGTGGGGCTCCCTGGGCACCCAGCAGTG3180.1233361258493903No Hit
GGGGAGAGCTGGGCTGGCGCTGCTCATGGAGTCGTAGGAGACA3020.11713053461168511No Hit
GTCTAGTGCCCATTTACTCTGGACTCCGGATGGCTGCCGCACG3010.11674268515932855No Hit
TATCTACGGAGATCAGCTGCATACTGGGCCATCTGCTCTGGTG2980.11557913680225883No Hit
GCTCATGGAGTCGTAGGAGACAGAAGGTGGCATTATAAGTCCA2950.1144155884451891No Hit
TCCATGAGCAGCGCCAGCCCAGCTCTCCCCTCTGCACCCTTGG2910.11286419063576282No Hit
CTGCTGTCCCCAGGGAGCTCAGCCCGCTGGACTTATAATGCCA2800.10859784665984051No Hit
GCTCTCCCCTCTGCACCCTTGGCTCTGGCCAAAGCTTGCTCCC2740.10627074994570107No Hit
AGGCAGAGGCGTGCGGCAGCCATCCGGAGTCCAGAGTAAATGG2730.1058829004933445No Hit
AGCCAAGGGTGCAGAGGGGAGAGCTGGGCTGGCGCTGCTCATG2640.10239225542213536No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAATTAT250.00548853529.635
GCCGTCT453.99242E-628.77777736
GCTTTAT2200.024.3863641
CGACCAT551.8952469E-523.54545410
ATGCGGC400.001927148423.12535
TATACAG400.001927148423.1255
GCGATGC502.694231E-422.232
TTTATTG2250.022.23
TATACAC1550.021.483873
CTTTATT2350.021.255322
CCGTCTT806.9171256E-720.812537
TACGACG450.003817882920.5555555
TAGGGTT450.003817882920.5555555
AAACGAT555.128235E-420.18181817
CGTAGTT555.128235E-420.1818182
CGATGCG555.128235E-420.18181833
GGCGATG555.128235E-420.18181831
TTATTGA2400.020.0416684
CCGACCA656.875624E-519.9230779
TAGTTCC759.2201335E-619.7333344
Produced by FastQC (version 0.11.5)