##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1630501.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	257832
Sequences flagged as poor quality	0
Sequence length	43
%GC	54
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.11832123243042	33.0	31.0	34.0	30.0	34.0
2	32.30578826522697	34.0	31.0	34.0	30.0	34.0
3	32.420537404201184	34.0	31.0	34.0	30.0	34.0
4	35.9813366843526	37.0	35.0	37.0	35.0	37.0
5	35.934348723199605	37.0	35.0	37.0	35.0	37.0
6	36.01062319650005	37.0	35.0	37.0	35.0	37.0
7	35.96142449346861	37.0	35.0	37.0	35.0	37.0
8	35.95397778398337	37.0	35.0	37.0	35.0	37.0
9	37.70476123987713	39.0	37.0	39.0	35.0	39.0
10	37.60689518756399	39.0	37.0	39.0	35.0	39.0
11	37.70162741630209	39.0	37.0	39.0	35.0	39.0
12	37.6411616866797	39.0	37.0	39.0	35.0	39.0
13	37.67667318253746	39.0	37.0	39.0	35.0	39.0
14	38.99006329703062	40.0	38.0	41.0	36.0	41.0
15	39.00995221694747	40.0	38.0	41.0	36.0	41.0
16	38.98416022836576	40.0	38.0	41.0	36.0	41.0
17	38.968886716931955	40.0	38.0	41.0	36.0	41.0
18	38.94409925843185	40.0	38.0	41.0	36.0	41.0
19	38.98454807781811	40.0	38.0	41.0	35.0	41.0
20	38.95946973222874	40.0	38.0	41.0	35.0	41.0
21	38.907854727109125	40.0	38.0	41.0	35.0	41.0
22	38.84337863414937	40.0	38.0	41.0	35.0	41.0
23	38.81104750380092	40.0	38.0	41.0	35.0	41.0
24	38.75595348909367	40.0	38.0	41.0	35.0	41.0
25	38.704606100096186	40.0	38.0	41.0	35.0	41.0
26	38.59200952558255	40.0	38.0	41.0	34.0	41.0
27	38.474293338297805	40.0	38.0	41.0	34.0	41.0
28	38.3714123925657	40.0	38.0	41.0	34.0	41.0
29	38.333825902137825	40.0	38.0	41.0	34.0	41.0
30	38.277227031555434	40.0	38.0	41.0	34.0	41.0
31	38.27032718979801	40.0	38.0	41.0	34.0	41.0
32	38.18200999100189	40.0	38.0	41.0	33.0	41.0
33	38.12363089143318	40.0	38.0	41.0	33.0	41.0
34	38.101438145769336	40.0	38.0	41.0	33.0	41.0
35	38.0523208911229	40.0	38.0	41.0	33.0	41.0
36	38.04530469452977	40.0	38.0	41.0	33.0	41.0
37	37.94791569704304	40.0	37.0	41.0	33.0	41.0
38	37.83520664618822	40.0	37.0	41.0	33.0	41.0
39	37.7877881721431	40.0	37.0	41.0	33.0	41.0
40	37.62800583325576	40.0	37.0	41.0	33.0	41.0
41	37.61814670017686	40.0	37.0	41.0	33.0	41.0
42	37.50471624934065	40.0	36.0	41.0	32.0	41.0
43	36.678585280337586	39.0	35.0	40.0	30.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	0.0
10	1.0
11	1.0
12	0.0
13	1.0
14	0.0
15	0.0
16	0.0
17	2.0
18	1.0
19	9.0
20	28.0
21	35.0
22	92.0
23	133.0
24	258.0
25	388.0
26	670.0
27	990.0
28	1515.0
29	2158.0
30	2932.0
31	3975.0
32	5232.0
33	7173.0
34	9773.0
35	13346.0
36	19128.0
37	30302.0
38	59067.0
39	100621.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.61471035402898	17.45865524837879	11.421390672996369	26.50524372459586
2	20.71852989543579	18.987557789568402	31.257563064320955	29.03634925067486
3	21.70754599894505	20.36287194762481	28.154767445468366	29.774814607961776
4	15.18818455428341	13.765552763039498	32.55608303081076	38.49017965186633
5	16.055415929752705	35.13023984610133	33.67851934593069	15.135824878215272
6	39.519919947873035	32.296999596636574	13.350165999565608	14.83291445592479
7	31.932421111421387	27.987604331502684	19.349421328617083	20.73055322845884
8	30.399252226255857	29.767445468366997	18.58690930528406	21.246393000093082
9	27.531105526078996	12.460051506407273	17.52071116075584	42.48813180675789
10	18.479475006981293	25.049256880449285	31.335908653696983	25.135359458872443
11	39.095612646994944	19.509991001892704	19.15743584970058	22.236960501411772
12	25.235812467032797	23.70109218405784	25.393279344689567	25.669816004219804
13	33.8034068695895	17.348506003909524	22.487899097086476	26.360188029414505
14	24.828570542058394	18.74747897855968	22.00735362561668	34.41659685376524
15	28.278491420770113	25.80478761363989	19.67948121257253	26.237239753017466
16	28.234276583201463	23.87252164199944	20.98071612522883	26.91248564957026
17	27.076933817369447	23.76431164479196	21.885568897576714	27.273185640261875
18	26.272534053181918	22.31840888640665	24.15565174221974	27.253405318191692
19	27.91662788172143	22.45687114089795	23.649508206894414	25.976992770486206
20	28.78269570883366	21.749821589251916	21.883629650314933	27.583853051599487
21	28.51779453287412	22.293974370908188	22.5600390952248	26.628192000992897
22	28.235052282106178	22.758618014831363	22.71711812342921	26.28921157963325
23	27.624189394644578	21.949176207763195	22.20787179248503	28.2187626051072
24	27.00673306649291	22.99287908405473	23.401284557386205	26.59910329206615
25	27.410872195848455	23.008005212696638	22.153185019702754	27.427937571752146
26	27.832464550560054	23.24187843246765	22.423128238542926	26.50252877842936
27	27.54196531074498	21.68272363399423	22.80632349747122	27.96898755778957
28	26.77596264234075	22.40257221756803	22.731468553166405	28.08999658692482
29	26.581262217257752	23.257392410561916	23.673167023488162	26.48817834869217
30	26.882621241738807	23.258555958918986	22.371156411927146	27.48766638741506
31	26.782168233578453	23.793788203171058	22.75784231592665	26.66620124732384
32	26.11933351950107	22.855968227372863	22.908327903441002	28.116370349685067
33	25.26722827267368	23.358233268174626	23.594821434112134	27.779717025039563
34	28.045006050451455	21.479490520959384	23.080145210834964	27.395358217754197
35	26.284945235657325	22.009292872878465	23.98383443482578	27.72192745663843
36	25.399097086474914	23.662695088274535	23.865540351857025	27.07266747339353
37	26.491281144311024	21.89991932731391	24.5838375376214	27.02496199075367
38	26.3012349126563	22.447174904589033	23.878727233237147	27.37286294951752
39	26.149973626237237	21.67923298892302	24.635421514784824	27.53537187005492
40	24.776210865990258	21.458934499984487	25.842796859970836	27.92205777405442
41	23.912857985044525	21.825064383009092	26.112740078811008	28.14933755313537
42	24.881705917031244	21.33249557851624	27.120760805485745	26.66503769896677
43	24.747897855968226	21.081944832293896	26.41138415712557	27.758773154612303
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	8.0
1	6.0
2	4.0
3	19.5
4	35.0
5	35.0
6	47.5
7	60.0
8	57.0
9	54.0
10	71.5
11	89.0
12	89.0
13	150.0
14	211.0
15	314.0
16	417.0
17	417.0
18	417.0
19	417.0
20	494.5
21	572.0
22	620.0
23	668.0
24	863.0
25	1058.0
26	1058.0
27	1343.5
28	1629.0
29	2070.5
30	2512.0
31	3018.0
32	3524.0
33	3524.0
34	4325.5
35	5127.0
36	6052.0
37	6977.0
38	7937.5
39	8898.0
40	8898.0
41	9646.5
42	10395.0
43	11222.0
44	12049.0
45	12936.5
46	13824.0
47	13824.0
48	15401.0
49	16978.0
50	17636.5
51	18295.0
52	19627.0
53	20959.0
54	20959.0
55	20546.5
56	20134.0
57	20601.0
58	21068.0
59	21565.5
60	22063.0
61	22063.0
62	20819.5
63	19576.0
64	18364.0
65	17152.0
66	15013.0
67	12874.0
68	12874.0
69	10877.5
70	8881.0
71	7487.5
72	6094.0
73	4382.0
74	2670.0
75	2670.0
76	1891.5
77	1113.0
78	889.0
79	665.0
80	525.5
81	386.0
82	386.0
83	285.5
84	185.0
85	143.5
86	102.0
87	84.5
88	67.0
89	67.0
90	49.0
91	31.0
92	18.5
93	6.0
94	5.5
95	5.0
96	5.0
97	2.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	257832.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	63.965428598305984
#Duplication Level	Percentage of deduplicated	Percentage of total
1	91.50491766223855	58.531512771177894
2	4.471488022696771	5.720412956879814
3	1.1828317609814334	2.2698102165259924
4	0.6369222451653351	1.6296401758318395
5	0.38366011314624304	1.2270491786737001
6	0.27762401093249744	1.0655003309086792
7	0.17212633267610983	0.7707094249877376
8	0.15488916954642737	0.7926041692218327
9	0.12323488108607474	0.7094494788238839
>10	0.8835588541277469	11.45079064963848
>50	0.12864637456219472	5.7097839790685425
>100	0.07828010527605245	9.37281806543631
>500	0.0018204675645593592	0.7499186028253177
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTGTGGGAGCAGGGAGCAAGCTTTGGCCAGAGCCAAGGGTGCA	687	0.26645257376896586	No Hit
ACCTAGGCCTGGTCAGCATGTTGATGTATCTACGGAGATCAGC	676	0.26218622979304357	No Hit
GCTTTATTGAGCCTGTGTGGGAGCAGGGAGCAAGCTTTGGCCA	569	0.2206863383908902	No Hit
CTTATACACATCTCCGAGCCCACGAGACTCGACGTCATCTCGT	500	0.19392472617828663	No Hit
AGTGTACCCAGGGGACAATGCCACACCAGAGCAGATGGCCCAG	446	0.17298085575103167	No Hit
GGCCTAGGTATGGGAAAAGACACAAAGAGGACACGCTGGCCTT	446	0.17298085575103167	No Hit
GCTGCATACTGGGCCATCTGCTCTGGTGTGGCATTGTCCCCTG	420	0.16289676998976077	No Hit
CCTCTGCACCCTTGGCTCTGGCCAAAGCTTGCTCCCTGCTCCC	404	0.1566911787520556	No Hit
GTATGCAGCTGATCTCCGTAGATACATCAACATGCTGACCAGG	398	0.15436408203791618	No Hit
GTGCAGAGGGGAGAGCTGGGCTGGCGCTGCTCATGGAGTCGTA	387	0.15009773806199384	No Hit
TCTCCGAGCCCACGAGACTCGACGTCATCTCGTATGCCGTCTT	384	0.14893418970492414	No Hit
CCTCTGCCTCTCCCTGCTGCTCCTGTCCACCTGCGTGGCTCTG	379	0.14699494244314126	No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA	372	0.14427999627664526	No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC	326	0.12643892146824287	No Hit
CCCTTGGCTCTGGCCAAAGCTTGCTCCCTGCTCCCACACAGGC	324	0.12566322256352974	No Hit
GGGTACACTGGCTCCAGTGGGGCTCCCTGGGCACCCAGCAGTG	318	0.1233361258493903	No Hit
GGGGAGAGCTGGGCTGGCGCTGCTCATGGAGTCGTAGGAGACA	302	0.11713053461168511	No Hit
GTCTAGTGCCCATTTACTCTGGACTCCGGATGGCTGCCGCACG	301	0.11674268515932855	No Hit
TATCTACGGAGATCAGCTGCATACTGGGCCATCTGCTCTGGTG	298	0.11557913680225883	No Hit
GCTCATGGAGTCGTAGGAGACAGAAGGTGGCATTATAAGTCCA	295	0.1144155884451891	No Hit
TCCATGAGCAGCGCCAGCCCAGCTCTCCCCTCTGCACCCTTGG	291	0.11286419063576282	No Hit
CTGCTGTCCCCAGGGAGCTCAGCCCGCTGGACTTATAATGCCA	280	0.10859784665984051	No Hit
GCTCTCCCCTCTGCACCCTTGGCTCTGGCCAAAGCTTGCTCCC	274	0.10627074994570107	No Hit
AGGCAGAGGCGTGCGGCAGCCATCCGGAGTCCAGAGTAAATGG	273	0.1058829004933445	No Hit
AGCCAAGGGTGCAGAGGGGAGAGCTGGGCTGGCGCTGCTCATG	264	0.10239225542213536	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	7.756989047131465E-4	0.0
3	0.0	0.0	0.0	0.0011635483570697199	0.0
4	0.0	0.0	0.0	0.0019392472617828663	0.0
5	0.0	3.8784945235657327E-4	0.0	0.0019392472617828663	0.0
6	0.0	3.8784945235657327E-4	0.0	0.0023270967141394397	0.0
7	0.0	3.8784945235657327E-4	0.0	0.003102795618852586	0.0
8	0.0	3.8784945235657327E-4	0.0	0.003102795618852586	0.0
9	0.0	3.8784945235657327E-4	0.0	0.0038784945235657326	0.0
10	0.0	3.8784945235657327E-4	0.0	0.005042042880635453	0.0
11	0.0	3.8784945235657327E-4	0.0	0.005429892332992026	0.0
12	0.0	3.8784945235657327E-4	0.0	0.005817741785348599	0.0
13	0.0	3.8784945235657327E-4	0.0	0.0065934406900617455	0.0
14	0.0	3.8784945235657327E-4	0.0	0.006981290142418319	0.0
15	0.0	3.8784945235657327E-4	0.0	0.008920537404201186	0.0
16	0.0	3.8784945235657327E-4	0.0	0.009308386856557759	0.0
17	0.0	3.8784945235657327E-4	0.0	0.011635483570697198	0.0
18	0.0	3.8784945235657327E-4	0.0	0.012799031927766918	0.0
19	0.0	3.8784945235657327E-4	0.0	0.01435042973719321	0.0
20	0.0	3.8784945235657327E-4	0.0	0.015126128641906357	0.0
21	0.0	3.8784945235657327E-4	0.0	0.018228924260758943	0.0
22	0.0	3.8784945235657327E-4	0.0	0.022883117689037825	0.0
23	0.0	3.8784945235657327E-4	0.0	0.03180365509323901	0.0
24	0.0	3.8784945235657327E-4	0.0	0.04266343975922306	0.0
25	0.0	3.8784945235657327E-4	0.0	0.046929783735145364	0.0
26	0.0	3.8784945235657327E-4	0.0	0.048869030996928235	0.0
27	0.0	3.8784945235657327E-4	0.0	0.058565267305842564	0.0
28	0.0	3.8784945235657327E-4	0.0	0.08959322349436842	0.0
29	0.0	3.8784945235657327E-4	0.0	0.13807440503894008	0.0
30	0.0	3.8784945235657327E-4	0.0	0.20517236029662725	0.0
31	0.0	3.8784945235657327E-4	0.0	0.4250829997828043	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CAATTAT	25	0.005488535	29.6	35
GCCGTCT	45	3.99242E-6	28.777777	36
GCTTTAT	220	0.0	24.386364	1
CGACCAT	55	1.8952469E-5	23.545454	10
ATGCGGC	40	0.0019271484	23.125	35
TATACAG	40	0.0019271484	23.125	5
GCGATGC	50	2.694231E-4	22.2	32
TTTATTG	225	0.0	22.2	3
TATACAC	155	0.0	21.48387	3
CTTTATT	235	0.0	21.25532	2
CCGTCTT	80	6.9171256E-7	20.8125	37
TACGACG	45	0.0038178829	20.555555	5
TAGGGTT	45	0.0038178829	20.555555	5
AAACGAT	55	5.128235E-4	20.181818	17
CGTAGTT	55	5.128235E-4	20.181818	2
CGATGCG	55	5.128235E-4	20.181818	33
GGCGATG	55	5.128235E-4	20.181818	31
TTATTGA	240	0.0	20.041668	4
CCGACCA	65	6.875624E-5	19.923077	9
TAGTTCC	75	9.2201335E-6	19.733334	4
>>END_MODULE