FastQCFastQC Report
Fri 10 Feb 2017
ERR1630500.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630500.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences742384
Sequences flagged as poor quality0
Sequence length43
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT171652.3121457358994806No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT170702.299349123903532No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT116431.568325825987629No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT92161.2414060647858791No Hit
CTTATACACATCTCCGAGCCCACGAGACATCTCAGGATCTCGT33920.45690639884480266No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25410.34227569559688786No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21530.2900116381818574No Hit
TCTCCGAGCCCACGAGACATCTCAGGATCTCGTATGCCGTCTT20120.27101877195629215TruSeq Adapter, Index 1 (95% over 22bp)
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT19140.2578180564236298No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17880.24084570788163537No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA17190.23155132653720983No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC14000.18858165046660488No Hit
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA13890.18709993749865297No Hit
ATACACATCTCCGAGCCCACGAGACATCTCAGGATCTCGTATG13800.18588762688851054No Hit
CTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCGAGATTGAGC13160.1772667514386086No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGAT13050.17578503847065668No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG12680.17080109485118214No Hit
CCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAATC12420.16729886419965947No Hit
TGCCTACCCTACGCCGGCAGGCGCGGGTAACCCGTTGAACCCC12330.16608655358951702No Hit
ACACATCTCCGAGCCCACGAGACATCTCAGGATCTCGTATGCC11940.16083320761223302No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11680.15733097696071036No Hit
CTACCATGGTGACCACGGGTGACGGGGAATCAGGGTTCGATTC11660.15706157460290093No Hit
GTACATGGGAATGGTATCAACGCAAAAAAAAAAAAAAAAAAAA11570.15584926399275847No Hit
CTCCGGAATCGAACCCTGATTCCCCGTCACCCGTGGTCACCAT11340.15275113687794997No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTT11030.1485754003319037No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATG11020.14844069915299898No Hit
TCTTAGAGGGACAAGTGGCGTTCAGCCACCCGAGATTGAGCAA10840.14601607793271407No Hit
ATTCCGGAGAGGGAGCCTGAGAAACGGCTACCACATCCAAGGA10340.1392810189874782No Hit
GCGCTACACTGACTGGCTCAGCGTGTGCCTACCCTACGCCGGC9980.13443177654690833No Hit
GGGTTCAACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGGCAC9650.12998663764305265No Hit
TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9610.12944783292743378No Hit
CATCTCCGAGCCCACGAGACATCTCAGGATCTCGTATGCCGTC9230.1243291881290545No Hit
ATTCGAACGTCTGCCCTATCAACTTTCGATGGTAGTCGCCGTG9140.12311687751891205No Hit
CCATGGTAGGCACGGCGACTACCATCGAAAGTTGATAGGGCAG9080.12230867044548373No Hit
CATCCAAGGAAGGCAGCAGGCGCGCAAATTACCCACTCCCGAC8300.11180197849091575No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG8000.10776094312377421No Hit
GGTCTGTGATGCCCTTAGATGTCCGGGGCTGCACGCGCGCTAC7930.10681803487144119No Hit
AGTCAGTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATC7740.10425871247225155No Hit
GAATGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA7660.1031811030410138No Hit
GTACATGGGAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAA7580.10210349360977608No Hit
CGTCTGCCCTATCAACTTTCGATGGTAGTCGCCGTGCCTACCA7510.10116058535744304No Hit
GCCCCGGACATCTAAGGGCATCACAGACCTGTTATTGCTCAAT7430.10008297592620531No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCG200.001841516137.08
CCGGTCG200.001841516137.09
TACAGGA251.233205E-436.9999962
TTAGATT303.5982614E-430.83333428
CTGGTAC250.005495106329.59999826
AACTGCG250.005495106329.5999987
GTATAGA250.005495106329.5999981
CGTAAGG250.005495106329.5999983
CCGTCTT2600.029.17307737
GCCGTCT2550.029.01960836
AATCGTC405.937339E-527.7528
GTATACC358.866426E-426.428571
CCGATTT400.001930546423.12521
TTAGAGT400.001930546423.1254
GTTAGAT400.001930546423.12527
TCGTCCC502.700854E-422.19999930
TGCCGTC3400.021.76470835
GATACAC603.723998E-521.5833343
GACACAT1550.021.4838726
GTGACAC1550.021.4838724
Produced by FastQC (version 0.11.5)