##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1630500.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	742384
Sequences flagged as poor quality	0
Sequence length	43
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.96913726588935	33.0	31.0	34.0	30.0	34.0
2	32.07499892239057	33.0	31.0	34.0	30.0	34.0
3	32.09502090562297	33.0	31.0	34.0	30.0	34.0
4	35.73457671501541	37.0	35.0	37.0	33.0	37.0
5	35.74068541347874	37.0	35.0	37.0	35.0	37.0
6	35.878298831871376	37.0	35.0	37.0	35.0	37.0
7	35.80419971335589	37.0	35.0	37.0	35.0	37.0
8	35.86552781309942	37.0	35.0	37.0	35.0	37.0
9	37.47726109398909	39.0	37.0	39.0	35.0	39.0
10	37.413880148279056	39.0	37.0	39.0	34.0	39.0
11	37.552443209982975	39.0	37.0	39.0	35.0	39.0
12	37.51958285738917	39.0	37.0	39.0	35.0	39.0
13	37.56898720877605	39.0	37.0	39.0	35.0	39.0
14	38.688538546089354	40.0	38.0	41.0	34.0	41.0
15	38.68117982068579	40.0	38.0	41.0	34.0	41.0
16	38.56707849307097	40.0	38.0	41.0	34.0	41.0
17	38.68255781374599	40.0	38.0	41.0	35.0	41.0
18	38.72881150455829	40.0	38.0	41.0	35.0	41.0
19	38.83349183172051	40.0	38.0	41.0	35.0	41.0
20	38.82322490786439	40.0	38.0	41.0	35.0	41.0
21	38.762912455009804	40.0	38.0	41.0	35.0	41.0
22	38.65247230543762	40.0	38.0	41.0	35.0	41.0
23	38.52316052070088	40.0	38.0	41.0	34.0	41.0
24	38.43649243518179	40.0	38.0	41.0	34.0	41.0
25	38.34564996012845	40.0	37.0	41.0	34.0	41.0
26	38.06940747645423	40.0	37.0	41.0	34.0	41.0
27	37.83891220715964	40.0	36.0	41.0	33.0	41.0
28	37.64809721114679	40.0	36.0	41.0	33.0	41.0
29	37.4779857863316	39.0	35.0	41.0	33.0	41.0
30	37.22716545615207	39.0	35.0	41.0	33.0	41.0
31	37.00164874242979	39.0	35.0	41.0	32.0	41.0
32	36.72162923769909	39.0	35.0	41.0	31.0	41.0
33	36.45018885105282	38.0	35.0	41.0	31.0	41.0
34	36.237453932196814	38.0	35.0	41.0	31.0	41.0
35	35.973715758960324	38.0	35.0	41.0	30.0	41.0
36	35.75999078643936	38.0	35.0	41.0	29.0	41.0
37	35.39265932455441	38.0	35.0	40.0	27.0	41.0
38	35.02839904954848	38.0	35.0	40.0	24.0	41.0
39	34.70804327679476	38.0	35.0	40.0	22.0	41.0
40	34.24497429901506	38.0	34.0	40.0	20.0	41.0
41	33.9041345179853	37.0	33.0	40.0	16.0	41.0
42	33.43630115950775	37.0	33.0	40.0	13.0	41.0
43	32.58886506174702	36.0	32.0	40.0	10.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	0.0
9	6.0
10	7.0
11	9.0
12	13.0
13	6.0
14	3.0
15	4.0
16	12.0
17	18.0
18	33.0
19	104.0
20	213.0
21	403.0
22	736.0
23	1289.0
24	2023.0
25	3247.0
26	4894.0
27	7189.0
28	10139.0
29	13433.0
30	16916.0
31	20106.0
32	24446.0
33	31195.0
34	39100.0
35	49056.0
36	67094.0
37	115997.0
38	149857.0
39	184835.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	47.357297571068344	19.8863391452402	13.321946593676588	19.43441669001487
2	19.125008082070735	22.87064376387422	33.41491734735663	24.58943080669842
3	22.827269984266902	27.42893165800987	29.442175477919786	20.301622879803443
4	13.32746934201168	16.904593849005366	31.25646565658743	38.51147115239553
5	11.934793853315805	42.52314166253583	31.07650488157072	14.46555960257764
6	31.06613289079506	31.937380115950774	18.236923209551932	18.759563783702234
7	25.852658462466866	34.14701825470377	21.619269811849392	18.381053470979978
8	32.150881484514755	29.48352873984353	19.612222246169097	18.753367529472616
9	28.59706028147158	11.184642987995431	22.891791848962264	37.32650488157072
10	20.62490570917477	29.6568891570939	28.730683850945066	20.98752128278627
11	30.698802775921898	24.040119399124983	19.837038513761073	25.424039311192054
12	21.515549904092758	31.791633440375872	26.88096726222548	19.81184939330589
13	30.451626112631736	19.635525550119613	29.52851893359771	20.38432940365094
14	22.53362141425462	23.41052608892433	29.869312916226644	24.18653958059441
15	27.236847776891743	26.26457466755749	27.456141296148623	19.042436259402145
16	22.35662406517382	25.060615530507125	30.361645725123388	22.221114679195672
17	20.822647039806892	28.988367206189793	29.045749908403195	21.14323584560012
18	19.43064505700554	23.07417724519925	33.72823228948899	23.766945408306213
19	19.760258841785383	26.768895881376753	34.73593180887519	18.73491346796267
20	20.72323756977521	23.36082135390849	36.66741201318994	19.24852906312636
21	22.195790857561583	25.048627125584606	34.03710209271751	18.718479924136297
22	22.629932757171492	25.543519256880536	32.77414383930688	19.05240414664109
23	20.469191146360913	25.083514730920925	33.52591111877411	20.92138300394405
24	18.843482618159875	28.00141166835492	33.46340977176232	19.69169594172288
25	18.972661048729496	26.665310674799024	34.10620379749564	20.25582447897584
26	20.153451583008255	27.827243044031118	33.19185219509041	18.82745317787021
27	19.779655811547663	27.961001314683504	33.55958641350029	18.699756460268542
28	18.47763421625466	27.608892433026572	34.32495851203689	19.588514838681867
29	18.26601866419535	28.46559193086058	35.098008577771076	18.170380827173
30	16.490253022694453	29.18826375568439	35.80936550356689	18.51211771805427
31	18.83162891441626	29.618095217569344	32.37030970495054	19.17996616306386
32	17.166722343153946	28.952401991422228	34.09111726545831	19.789758399965514
33	16.72961701760814	29.1000344835018	33.88515916291299	20.28518933597707
34	18.383208689842455	29.321752623978963	33.51459621974612	18.780442466432465
35	17.503609991594647	29.902584107416107	33.917891549386844	18.675914351602405
36	18.00995172309748	29.77717730985582	32.181189249768316	20.031681717278392
37	17.734487812237333	28.921016616737425	33.27577102954805	20.068724541477188
38	17.478824974676176	27.96517705122955	33.03708054052889	21.51891743356538
39	18.10532015776202	28.898790922218154	33.112243798357724	19.883645121662106
40	16.745915860255607	28.90822000474148	33.49937498652989	20.846489148473026
41	17.160660790103236	28.93987478178409	32.71150779111619	21.18795663699649
42	16.77851354555055	29.610417250371775	33.45492359749132	20.15614560658635
43	15.99387917843057	28.122238625832452	32.93093601155198	22.952946184185006
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	994.0
1	732.5
2	471.0
3	1362.0
4	2253.0
5	2253.0
6	3447.5
7	4642.0
8	4803.5
9	4965.0
10	7185.0
11	9405.0
12	9405.0
13	16450.0
14	23495.0
15	31741.0
16	39987.0
17	35191.0
18	30395.0
19	30395.0
20	32252.0
21	34109.0
22	24248.5
23	14388.0
24	13278.0
25	12168.0
26	12168.0
27	12545.5
28	12923.0
29	13807.0
30	14691.0
31	15716.0
32	16741.0
33	16741.0
34	18845.0
35	20949.0
36	22067.0
37	23185.0
38	26985.5
39	30786.0
40	30786.0
41	33471.0
42	36156.0
43	38476.0
44	40796.0
45	42982.5
46	45169.0
47	45169.0
48	47640.5
49	50112.0
50	49882.0
51	49652.0
52	45245.5
53	40839.0
54	40839.0
55	38999.5
56	37160.0
57	33939.5
58	30719.0
59	28929.0
60	27139.0
61	27139.0
62	25358.5
63	23578.0
64	21777.0
65	19976.0
66	18805.5
67	17635.0
68	17635.0
69	13909.0
70	10183.0
71	8958.5
72	7734.0
73	5699.5
74	3665.0
75	3665.0
76	2912.0
77	2159.0
78	1602.5
79	1046.0
80	855.5
81	665.0
82	665.0
83	597.5
84	530.0
85	496.5
86	463.0
87	363.0
88	263.0
89	263.0
90	213.5
91	164.0
92	97.5
93	31.0
94	16.0
95	1.0
96	1.0
97	1.5
98	2.0
99	1.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	742384.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	29.17737581220213
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.75586556337653	22.39534735333501
2	10.166881723240001	5.932858577543653
3	3.655135960309059	3.1994182667589515
4	1.9262051360119596	2.2480644457925947
5	1.2293411369944618	1.7934474177743638
6	0.8834625318664986	1.5466270984961057
7	0.6546987973376837	1.3371674997602845
8	0.51349829058354	1.1986026082623458
9	0.40195909704062793	1.0555300471939044
>10	3.1158154471861264	18.191113120365003
>50	0.3865769199400901	7.884690578888959
>100	0.27440472014798556	16.35498510637303
>500	0.023176074336823105	4.5160478183412565
>1k	0.011124515681675091	4.894999842388699
>5k	4.635214867364621E-4	1.246403231128205
>10k+	0.0013905644602093864	6.204696987597633
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	17165	2.3121457358994806	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	17070	2.299349123903532	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	11643	1.568325825987629	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	9216	1.2414060647858791	No Hit
CTTATACACATCTCCGAGCCCACGAGACATCTCAGGATCTCGT	3392	0.45690639884480266	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2541	0.34227569559688786	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2153	0.2900116381818574	No Hit
TCTCCGAGCCCACGAGACATCTCAGGATCTCGTATGCCGTCTT	2012	0.27101877195629215	TruSeq Adapter, Index 1 (95% over 22bp)
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1914	0.2578180564236298	No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1788	0.24084570788163537	No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA	1719	0.23155132653720983	No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC	1400	0.18858165046660488	No Hit
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA	1389	0.18709993749865297	No Hit
ATACACATCTCCGAGCCCACGAGACATCTCAGGATCTCGTATG	1380	0.18588762688851054	No Hit
CTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCGAGATTGAGC	1316	0.1772667514386086	No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGAT	1305	0.17578503847065668	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG	1268	0.17080109485118214	No Hit
CCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAATC	1242	0.16729886419965947	No Hit
TGCCTACCCTACGCCGGCAGGCGCGGGTAACCCGTTGAACCCC	1233	0.16608655358951702	No Hit
ACACATCTCCGAGCCCACGAGACATCTCAGGATCTCGTATGCC	1194	0.16083320761223302	No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1168	0.15733097696071036	No Hit
CTACCATGGTGACCACGGGTGACGGGGAATCAGGGTTCGATTC	1166	0.15706157460290093	No Hit
GTACATGGGAATGGTATCAACGCAAAAAAAAAAAAAAAAAAAA	1157	0.15584926399275847	No Hit
CTCCGGAATCGAACCCTGATTCCCCGTCACCCGTGGTCACCAT	1134	0.15275113687794997	No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTT	1103	0.1485754003319037	No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATG	1102	0.14844069915299898	No Hit
TCTTAGAGGGACAAGTGGCGTTCAGCCACCCGAGATTGAGCAA	1084	0.14601607793271407	No Hit
ATTCCGGAGAGGGAGCCTGAGAAACGGCTACCACATCCAAGGA	1034	0.1392810189874782	No Hit
GCGCTACACTGACTGGCTCAGCGTGTGCCTACCCTACGCCGGC	998	0.13443177654690833	No Hit
GGGTTCAACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGGCAC	965	0.12998663764305265	No Hit
TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	961	0.12944783292743378	No Hit
CATCTCCGAGCCCACGAGACATCTCAGGATCTCGTATGCCGTC	923	0.1243291881290545	No Hit
ATTCGAACGTCTGCCCTATCAACTTTCGATGGTAGTCGCCGTG	914	0.12311687751891205	No Hit
CCATGGTAGGCACGGCGACTACCATCGAAAGTTGATAGGGCAG	908	0.12230867044548373	No Hit
CATCCAAGGAAGGCAGCAGGCGCGCAAATTACCCACTCCCGAC	830	0.11180197849091575	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG	800	0.10776094312377421	No Hit
GGTCTGTGATGCCCTTAGATGTCCGGGGCTGCACGCGCGCTAC	793	0.10681803487144119	No Hit
AGTCAGTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATC	774	0.10425871247225155	No Hit
GAATGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA	766	0.1031811030410138	No Hit
GTACATGGGAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAA	758	0.10210349360977608	No Hit
CGTCTGCCCTATCAACTTTCGATGGTAGTCGCCGTGCCTACCA	751	0.10116058535744304	No Hit
GCCCCGGACATCTAAGGGCATCACAGACCTGTTATTGCTCAAT	743	0.10008297592620531	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	4.041035367141533E-4	0.0
3	0.0	0.0	0.0	6.735058945235889E-4	0.0
4	0.0	0.0	0.0	6.735058945235889E-4	0.0
5	0.0	0.0	0.0	6.735058945235889E-4	0.0
6	0.0	0.0	0.0	6.735058945235889E-4	0.0
7	0.0	0.0	0.0	6.735058945235889E-4	0.0
8	0.0	0.0	0.0	8.082070734283066E-4	0.0
9	0.0	0.0	0.0	0.0014817129679518955	0.0
10	0.0	0.0	0.0	0.0021552188624754842	0.0
11	0.0	0.0	0.0	0.0033675294726179445	0.0
12	0.0	0.0	0.0	0.0060615530507123	0.0
13	0.0	0.0	0.0	0.008082070734283067	0.0
14	0.0	0.0	0.0	0.011045496670186858	0.0
15	0.0	0.0	0.0	0.01710704972089916	0.0
16	0.0	0.0	0.0	0.03434880062070303	0.0
17	0.0	0.0	0.0	0.06277074936959848	0.0
18	0.0	0.0	0.0	0.07166102717730985	0.0
19	0.0	0.0	0.0	0.09065389340287507	0.0
20	0.0	0.0	0.0	0.10183409125196664	0.0
21	0.0	0.0	0.0	0.12998663764305265	0.0
22	0.0	0.0	0.0	0.19235328347593697	0.0
23	0.0	0.0	0.0	0.28906872992952437	0.0
24	0.0	0.0	0.0	0.4536735705510895	0.0
25	0.0	0.0	0.0	0.48088320868984247	0.0
26	0.0	0.0	0.0	0.5308573460634928	0.0
27	0.0	0.0	0.0	0.5755781374598591	0.0
28	0.0	0.0	0.0	0.6530313153300717	0.0
29	0.0	0.0	0.0	0.7656415008944158	0.0
30	0.0	0.0	0.0	0.9714649022608246	0.0
31	0.0	0.0	0.0	1.5455613267527317	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCG	20	0.0018415161	37.0	8
CCGGTCG	20	0.0018415161	37.0	9
TACAGGA	25	1.233205E-4	36.999996	2
TTAGATT	30	3.5982614E-4	30.833334	28
CTGGTAC	25	0.0054951063	29.599998	26
AACTGCG	25	0.0054951063	29.599998	7
GTATAGA	25	0.0054951063	29.599998	1
CGTAAGG	25	0.0054951063	29.599998	3
CCGTCTT	260	0.0	29.173077	37
GCCGTCT	255	0.0	29.019608	36
AATCGTC	40	5.937339E-5	27.75	28
GTATACC	35	8.866426E-4	26.42857	1
CCGATTT	40	0.0019305464	23.125	21
TTAGAGT	40	0.0019305464	23.125	4
GTTAGAT	40	0.0019305464	23.125	27
TCGTCCC	50	2.700854E-4	22.199999	30
TGCCGTC	340	0.0	21.764708	35
GATACAC	60	3.723998E-5	21.583334	3
GACACAT	155	0.0	21.48387	26
GTGACAC	155	0.0	21.48387	24
>>END_MODULE