FastQCFastQC Report
Fri 10 Feb 2017
ERR1630499.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630499.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences982719
Sequences flagged as poor quality0
Sequence length43
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTAGGCCTGGTCAGCATGTTGATGTATCTACGGAGATCAGC25800.2625369001718701No Hit
GTGTGGGAGCAGGGAGCAAGCTTTGGCCAGAGCCAAGGGTGCA20990.21359106723285087No Hit
GCTTTATTGAGCCTGTGTGGGAGCAGGGAGCAAGCTTTGGCCA19640.19985367129362513No Hit
GGCCTAGGTATGGGAAAAGACACAAAGAGGACACGCTGGCCTT18540.18866023756536712No Hit
AGTGTACCCAGGGGACAATGCCACACCAGAGCAGATGGCCCAG14180.14429353660608984No Hit
GTATGCAGCTGATCTCCGTAGATACATCAACATGCTGACCAGG13440.13676340846162535No Hit
CCTCTGCCTCTCCCTGCTGCTCCTGTCCACCTGCGTGGCTCTG13420.13655989148474793No Hit
GGGTACACTGGCTCCAGTGGGGCTCCCTGGGCACCCAGCAGTG13330.13564406508879953No Hit
GCTGCATACTGGGCCATCTGCTCTGGTGTGGCATTGTCCCCTG12050.12261897856864475No Hit
GTCTAGTGCCCATTTACTCTGGACTCCGGATGGCTGCCGCACG11830.12038029182299315No Hit
CCTCTGCACCCTTGGCTCTGGCCAAAGCTTGCTCCCTGCTCCC11450.1165134692623222No Hit
AGGCAGAGGCGTGCGGCAGCCATCCGGAGTCCAGAGTAAATGG11020.11213785425945769No Hit
GTGCAGAGGGGAGAGCTGGGCTGGCGCTGCTCATGGAGTCGTA10440.10623586193001255No Hit
CTGTTACTACAGCCACTGCTGGGTGCCCAGGGAGCCCCACTGG10440.10623586193001255No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA10300.10481124309187062No Hit
CCCTTGGCTCTGGCCAAAGCTTGCTCCCTGCTCCCACACAGGC9970.1014532129733932No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTTAT551.9022946E-523.5454541
TCTATAC502.7017153E-422.23
CGAAATT603.7255784E-521.58333420
GGTATCA6950.020.762591
AATACGC450.003825419820.5555555
TCGATTT609.2355156E-418.513
ATTATAC752.0668822E-417.2666663
GCTTTAT5150.016.8834951
ACGCTGA3550.016.67605611
TTTGCGC1005.8760434E-616.65000217
CCGAAAT904.444902E-516.44444519
TACTCCG1251.656881E-716.2799995
TCTGTCG803.3819414E-416.18758
CGGCTAA803.3819414E-416.187526
ACCTAGG5700.015.9035081
TCTTATA8850.015.88700537
GCAGTCG1059.340258E-615.8571449
AAACGCT1302.5876398E-715.65384629
CAGTCGG1302.5876398E-715.65384610
GTATTAT855.363169E-415.2352931
Produced by FastQC (version 0.11.5)