FastQCFastQC Report
Fri 10 Feb 2017
ERR1630498.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630498.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1796660
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT71770.39946344884396606No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT70170.39055803546580875No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT59280.329945565660726No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT38280.21306201507241213No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCCAT3550.020.32394413
TACGCAT500.00703638618.55
TTCGGGC1950.018.02564235
GGTATCA31950.017.3129881
AGTCGGT3150.017.03174611
GTCGCCA4150.016.93975812
CGAATTA1554.0199666E-1016.70967915
ACCGTCC904.4482003E-516.4444458
CGAACGA1851.8189894E-1116.016
ACGAACG1902.7284841E-1115.57894815
CCGCTTA1201.93688E-615.41666625
TAGGACA6150.015.3414634
TCGGTGA3500.015.32857113
GAACCGT855.366238E-415.2352946
CGGTGAT3650.015.2054814
GCAGTCG3550.015.1126779
GTCGGTG3450.015.01449312
CTTATAC11750.014.837
ACGGACC2650.014.6603778
TTAGGAC6100.014.5573773
Produced by FastQC (version 0.11.5)