FastQCFastQC Report
Fri 10 Feb 2017
ERR1630497.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630497.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1012736
Sequences flagged as poor quality0
Sequence length43
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT148061.4619802199191103No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT136601.3488214105156724No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT107121.0577287664307382No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT76570.7560706837714863No Hit
GGATTACTCCGGTCTGAACTCAGATCACGTAGGACTTTAATCG21940.2166408619817998No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21750.21476475606673406No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA18780.18543825834175934No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT17530.17309545626895856No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC16810.16598600227502527No Hit
GAGTAATCCAGGTCGGTTTCTATCTACTTCAAATTCCTCCCTG16800.16588725985844288No Hit
CTACTATACTCAATTGATCCAATAACTTGACCAACGGAACAAG16780.16568977502527804No Hit
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA16310.16104888144590496No Hit
CTCTAATACTGGTGATGCTAGAGGTGATGTTTTTGGTAAACAG16160.15956774519716885No Hit
CTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCGAGATTGAGC15340.15147086703741153No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15090.14900230662285135No Hit
GTCCTAAACTACTAAACCTGCATTAAAAATTTCGGTTGGGGCG14870.14682997345803844No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCC14770.14584254929221435No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14530.1434727312942366No Hit
AGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCC14280.14100417087967645No Hit
GTCCTGATCCAACATCGAGGTCGTAAACCCTATTGTTGATATG13860.13685698938321536No Hit
GTACATGGGAATGGTATCAACGCAAAAAAAAAAAAAAAAAAAA13780.13606705005055614No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTC13730.1355733379676441No Hit
TGCCTACCCTACGCCGGCAGGCGCGGGTAACCCGTTGAACCCC13410.13241358063700706No Hit
ACGTAGGACTTTAATCGTTGAACAAACGAACCTTTAATAGCGG13190.13024124747219415No Hit
GATAGAAACCGACCTGGATTACTCCGGTCTGAACTCAGATCAC13180.13014250505561173No Hit
GATCAGGACATCCCGATGGTGCAGCCGCTATTAAAGGTTCGTT12850.1268840053083923No Hit
ATTCTAGAGTCCATATCAACAATAGGGTTTACGACCTCGATGT12790.12629155080889787No Hit
TCTTAGAGGGACAAGTGGCGTTCAGCCACCCGAGATTGAGCAA12620.12461292972699696No Hit
TGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTCAATTG12300.12145317239635996No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATG12170.12016952098078867No Hit
GTTATCCCTAGGGTAACTTGTTCCGTTGGTCAAGTTATTGGAT11950.11799718781597573No Hit
GTACGGGGGAAGGCGCTTTGTGAAGTAGGCCTTATTTCTCTTG11910.1176022181496461No Hit
GGGTTCAACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGGCAC11790.11641730915065723No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG11680.11533114256825076No Hit
CTATTAAAGGTTCGTTTGTTCAACGATTAAAGTCCTACGTGAT11550.11404749115267948No Hit
GTCTTAGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGT11500.11355377906976745No Hit
AGGGATAACAGCGCAATCCTATTCTAGAGTCCATATCAACAAT11390.11246761248736098No Hit
ATCCTATTCTAGAGTCCATATCAACAATAGGGTTTACGACCTC11110.10970282482305359No Hit
GTCCTACGTGATCTGAGTTCAGACCGGAGTAATCCAGGTCGGT11100.10960408240647118No Hit
GGTCTGAACTCAGATCACGTAGGACTTTAATCGTTGAACAAAC10460.10328456774519716No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10380.10249462841253791No Hit
CTTATACACATCTCCGAGCCCACGAGACCGATCAGTATCTCGT10230.10101349216380183No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTATAG200.001841832337.036
CTTAGAA352.3880297E-531.7142872
CTCTATA303.599179E-430.8333342
CGTTATA405.9394417E-527.7500022
TTAGAAC358.868663E-426.4285723
ATCCGAC358.868663E-426.42857227
GTCGTAG358.868663E-426.42857213
TATAATC451.3230607E-424.6666663
TGCGGAC400.001931029123.12500213
TCATGGA400.001931029123.1250022
GGTATGC400.001931029123.12500230
CCTTGCT1054.0017767E-1122.90476415
CACTGCG502.7017953E-422.1999999
ACTCGCA855.1792085E-821.76470621
AGTCCTT1201.0913936E-1121.58333412
AATCCGA450.003825499420.55555526
TCCGTAT909.4754796E-820.55555513
GGACCGC450.003825499420.5555556
TATACGA450.003825499420.55555536
ACTTAGA555.1425135E-420.1818181
Produced by FastQC (version 0.11.5)