##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1630497.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1012736
Sequences flagged as poor quality	0
Sequence length	43
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.02960100164307	33.0	31.0	34.0	30.0	34.0
2	32.15935051188069	33.0	31.0	34.0	30.0	34.0
3	32.22807128412538	34.0	31.0	34.0	30.0	34.0
4	35.83177649456522	37.0	35.0	37.0	35.0	37.0
5	35.82027695273003	37.0	35.0	37.0	35.0	37.0
6	35.93799469950708	37.0	35.0	37.0	35.0	37.0
7	35.87862483411274	37.0	35.0	37.0	35.0	37.0
8	35.915638428968656	37.0	35.0	37.0	35.0	37.0
9	37.574503128159755	39.0	37.0	39.0	35.0	39.0
10	37.48518567524014	39.0	37.0	39.0	35.0	39.0
11	37.605947650720424	39.0	37.0	39.0	35.0	39.0
12	37.56533884447674	39.0	37.0	39.0	35.0	39.0
13	37.60533841001011	39.0	37.0	39.0	35.0	39.0
14	38.82336265324823	40.0	38.0	41.0	35.0	41.0
15	38.80628416487614	40.0	38.0	41.0	35.0	41.0
16	38.73483908935794	40.0	38.0	41.0	35.0	41.0
17	38.78934589073559	40.0	38.0	41.0	35.0	41.0
18	38.8281220377275	40.0	38.0	41.0	35.0	41.0
19	38.93259151447169	40.0	38.0	41.0	35.0	41.0
20	38.890995284062186	40.0	38.0	41.0	35.0	41.0
21	38.85015147086704	40.0	38.0	41.0	35.0	41.0
22	38.75727929095046	40.0	38.0	41.0	35.0	41.0
23	38.67380738909252	40.0	38.0	41.0	35.0	41.0
24	38.61309166456016	40.0	38.0	41.0	34.0	41.0
25	38.52879822579626	40.0	38.0	41.0	34.0	41.0
26	38.326887757520225	40.0	37.0	41.0	34.0	41.0
27	38.15607720077098	40.0	37.0	41.0	34.0	41.0
28	38.00429134542467	40.0	37.0	41.0	33.0	41.0
29	37.905523255813954	40.0	37.0	41.0	33.0	41.0
30	37.71128902300303	40.0	37.0	41.0	33.0	41.0
31	37.57426910863246	40.0	36.0	41.0	33.0	41.0
32	37.392295721688576	40.0	35.0	41.0	33.0	41.0
33	37.22440695304601	39.0	35.0	41.0	32.0	41.0
34	37.09387540286906	39.0	35.0	41.0	32.0	41.0
35	36.91332390672397	39.0	35.0	41.0	31.0	41.0
36	36.792369383531344	39.0	35.0	41.0	31.0	41.0
37	36.5360952903817	39.0	35.0	41.0	31.0	41.0
38	36.27147153848584	39.0	35.0	41.0	30.0	41.0
39	36.07914698401163	39.0	35.0	41.0	30.0	41.0
40	35.76124083670374	39.0	35.0	41.0	27.0	41.0
41	35.555766754613245	39.0	35.0	41.0	26.0	41.0
42	35.23438092454499	39.0	35.0	41.0	24.0	41.0
43	34.431222944577854	38.0	34.0	40.0	21.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	3.0
10	16.0
11	10.0
12	10.0
13	4.0
14	2.0
15	3.0
16	9.0
17	10.0
18	33.0
19	76.0
20	164.0
21	353.0
22	586.0
23	1079.0
24	1825.0
25	2888.0
26	4745.0
27	7138.0
28	10086.0
29	14270.0
30	18217.0
31	22664.0
32	27998.0
33	36054.0
34	47032.0
35	59474.0
36	83062.0
37	140724.0
38	220528.0
39	313672.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	46.611061520475225	19.942413422649143	12.380027963852376	21.066497093023255
2	18.966147149898887	21.880430832912033	34.8228956016178	24.330526415571285
3	21.06886691102123	27.401711798533874	29.038959807886755	22.49046148255814
4	12.665788517441861	16.327354809150656	34.78122630182002	36.225630371587464
5	11.591767252275027	41.75727929095046	33.86341553968656	12.787537917087969
6	31.497053526289182	35.26940880940344	17.18216790950455	16.05136975480283
7	27.18013381572295	32.518741310667345	22.84869896991911	17.452425903690596
8	29.651162790697676	31.474638997724973	19.535298439079877	19.338899772497474
9	27.58349658746208	12.197650720424672	21.981049355409503	38.23780333670374
10	19.339492226996967	28.420239825581394	31.11413043478261	21.12613751263903
11	32.0205858190091	24.489699191102122	20.805027173913043	22.68468781597573
12	21.8233577161274	30.121374178463096	27.283319641051566	20.771948464357937
13	28.761888586956523	20.850646170374116	28.414611507836195	21.972853734833166
14	22.503001769464102	22.22217833670374	28.35200881572295	26.9228110781092
15	26.26340922017189	27.119999684024265	26.894175777300305	19.72241531850354
16	23.222636501516682	25.83832311678463	28.50081363751264	22.438226744186046
17	21.781589673913043	28.039884036905967	27.924651636754298	22.253874652426695
18	19.78985638902932	24.993976712588474	31.870892315470172	23.345274582912033
19	21.09088646991911	25.915440944135494	32.40716237992922	20.58651020601618
20	21.471143516177957	24.521395506825076	33.49431638650152	20.51314459049545
21	22.127484359201212	26.267457659251768	31.590068882709808	20.014989098837212
22	23.3611721119818	25.66414149393327	30.146948464357937	20.827737929726997
23	21.393433234327603	26.141264850859454	31.63845266683519	20.826849247977755
24	20.056559656218404	28.125296227249745	29.775084523508593	22.043059593023255
25	20.984145917593526	26.583235907482305	31.67676472446916	20.755853450455007
26	20.66530665444894	28.003546827603643	31.12479461577351	20.206351902173914
27	21.36213188827098	27.568685224974722	30.30789860338726	20.761284283367036
28	19.9555461640546	27.197018768958543	32.7715218971183	20.075913169868553
29	20.72484833164813	27.607392252275027	32.334092991658245	19.333666424418606
30	18.794927799544993	28.51671116658241	32.348608126895854	20.339752906976745
31	20.24328156597573	29.064534093781596	30.638784441354904	20.053399898887765
32	19.01749320652174	28.16943408746208	31.340151826339735	21.47292087967644
33	18.425433676693633	28.466253791708795	31.71685414560162	21.391458385995957
34	20.499320652173914	27.79885379802831	31.678739572800808	20.023085976996967
35	19.199672965116278	29.108276984327603	31.19707406471183	20.494975985844288
36	19.84801567239636	29.118151225985844	29.79493174924166	21.23890135237614
37	19.67106926188069	27.41267220677452	31.419540729271993	21.4967178020728
38	19.28380150404449	27.297044836956523	31.203294836956523	22.215858822042467
39	19.250920279322546	28.60004976617796	31.367700960566232	20.781328993933265
40	18.749605030333672	28.49103813827098	31.194802989130434	21.564553842264914
41	18.867997187815977	28.014408493427705	31.110180738119315	22.007413580637007
42	18.25470803842265	29.051302609959556	31.44284393958544	21.251145412032358
43	18.658367037411526	26.99291819388271	30.971447642821033	23.37726712588473
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	134.0
1	196.0
2	258.0
3	1137.0
4	2016.0
5	2016.0
6	3058.0
7	4100.0
8	4168.0
9	4236.0
10	5962.0
11	7688.0
12	7688.0
13	13263.5
14	18839.0
15	26328.0
16	33817.0
17	29733.0
18	25649.0
19	25649.0
20	26498.5
21	27348.0
22	19577.5
23	11807.0
24	11524.0
25	11241.0
26	11241.0
27	12455.5
28	13670.0
29	16048.5
30	18427.0
31	22316.0
32	26205.0
33	26205.0
34	32377.0
35	38549.0
36	42342.0
37	46135.0
38	55018.5
39	63902.0
40	63902.0
41	68004.5
42	72107.0
43	78201.5
44	84296.0
45	81736.5
46	79177.0
47	79177.0
48	80655.0
49	82133.0
50	78885.0
51	75637.0
52	69211.5
53	62786.0
54	62786.0
55	56187.5
56	49589.0
57	40616.5
58	31644.0
59	30226.5
60	28809.0
61	28809.0
62	27274.5
63	25740.0
64	23290.5
65	20841.0
66	19740.5
67	18640.0
68	18640.0
69	14782.5
70	10925.0
71	9337.5
72	7750.0
73	5644.5
74	3539.0
75	3539.0
76	2797.0
77	2055.0
78	1600.0
79	1145.0
80	862.0
81	579.0
82	579.0
83	540.0
84	501.0
85	460.5
86	420.0
87	331.0
88	242.0
89	242.0
90	188.0
91	134.0
92	78.5
93	23.0
94	12.5
95	2.0
96	2.0
97	1.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1012736.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	21.65787966249369
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.89577287107207	16.004257564086448
2	10.434566622144859	4.519811764653732
3	3.951973835310162	2.5677412126341346
4	2.1611631621619662	1.8722484678847278
5	1.3714511822081865	1.4851362333624805
6	1.005722345289409	1.3069088116895369
7	0.7183259840611493	1.0890192404867112
8	0.5963614388818151	1.033273942292315
9	0.491860023977927	0.9587380689094683
>10	4.085245851949671	18.453941509907946
>50	0.668961704767904	10.088849316520406
>100	0.5485394403060696	23.06008534157237
>500	0.05036672657234362	7.553051114402204
>1k	0.01785729396655819	5.362453082272686
>5k	4.578793324758511E-4	0.7593213731107158
>10k+	0.0013736379974275533	3.885162956214134
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	14806	1.4619802199191103	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	13660	1.3488214105156724	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	10712	1.0577287664307382	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	7657	0.7560706837714863	No Hit
GGATTACTCCGGTCTGAACTCAGATCACGTAGGACTTTAATCG	2194	0.2166408619817998	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2175	0.21476475606673406	No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA	1878	0.18543825834175934	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1753	0.17309545626895856	No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC	1681	0.16598600227502527	No Hit
GAGTAATCCAGGTCGGTTTCTATCTACTTCAAATTCCTCCCTG	1680	0.16588725985844288	No Hit
CTACTATACTCAATTGATCCAATAACTTGACCAACGGAACAAG	1678	0.16568977502527804	No Hit
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA	1631	0.16104888144590496	No Hit
CTCTAATACTGGTGATGCTAGAGGTGATGTTTTTGGTAAACAG	1616	0.15956774519716885	No Hit
CTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCGAGATTGAGC	1534	0.15147086703741153	No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1509	0.14900230662285135	No Hit
GTCCTAAACTACTAAACCTGCATTAAAAATTTCGGTTGGGGCG	1487	0.14682997345803844	No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCC	1477	0.14584254929221435	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1453	0.1434727312942366	No Hit
AGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCC	1428	0.14100417087967645	No Hit
GTCCTGATCCAACATCGAGGTCGTAAACCCTATTGTTGATATG	1386	0.13685698938321536	No Hit
GTACATGGGAATGGTATCAACGCAAAAAAAAAAAAAAAAAAAA	1378	0.13606705005055614	No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTC	1373	0.1355733379676441	No Hit
TGCCTACCCTACGCCGGCAGGCGCGGGTAACCCGTTGAACCCC	1341	0.13241358063700706	No Hit
ACGTAGGACTTTAATCGTTGAACAAACGAACCTTTAATAGCGG	1319	0.13024124747219415	No Hit
GATAGAAACCGACCTGGATTACTCCGGTCTGAACTCAGATCAC	1318	0.13014250505561173	No Hit
GATCAGGACATCCCGATGGTGCAGCCGCTATTAAAGGTTCGTT	1285	0.1268840053083923	No Hit
ATTCTAGAGTCCATATCAACAATAGGGTTTACGACCTCGATGT	1279	0.12629155080889787	No Hit
TCTTAGAGGGACAAGTGGCGTTCAGCCACCCGAGATTGAGCAA	1262	0.12461292972699696	No Hit
TGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTCAATTG	1230	0.12145317239635996	No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATG	1217	0.12016952098078867	No Hit
GTTATCCCTAGGGTAACTTGTTCCGTTGGTCAAGTTATTGGAT	1195	0.11799718781597573	No Hit
GTACGGGGGAAGGCGCTTTGTGAAGTAGGCCTTATTTCTCTTG	1191	0.1176022181496461	No Hit
GGGTTCAACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGGCAC	1179	0.11641730915065723	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG	1168	0.11533114256825076	No Hit
CTATTAAAGGTTCGTTTGTTCAACGATTAAAGTCCTACGTGAT	1155	0.11404749115267948	No Hit
GTCTTAGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGT	1150	0.11355377906976745	No Hit
AGGGATAACAGCGCAATCCTATTCTAGAGTCCATATCAACAAT	1139	0.11246761248736098	No Hit
ATCCTATTCTAGAGTCCATATCAACAATAGGGTTTACGACCTC	1111	0.10970282482305359	No Hit
GTCCTACGTGATCTGAGTTCAGACCGGAGTAATCCAGGTCGGT	1110	0.10960408240647118	No Hit
GGTCTGAACTCAGATCACGTAGGACTTTAATCGTTGAACAAAC	1046	0.10328456774519716	No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1038	0.10249462841253791	No Hit
CTTATACACATCTCCGAGCCCACGAGACCGATCAGTATCTCGT	1023	0.10101349216380183	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.001086166582406471	0.0
3	0.0	0.0	0.0	0.0014811362487360971	0.0
4	0.0	0.0	0.0	0.0029622724974721942	0.0
5	0.0	0.0	0.0	0.00335724216380182	0.0
6	0.0	0.0	0.0	0.00335724216380182	9.874241658240647E-5
7	0.0	0.0	0.0	0.0038509542467138522	9.874241658240647E-5
8	0.0	0.0	0.0	0.004147181496461072	9.874241658240647E-5
9	0.0	0.0	0.0	0.0065169994944388274	9.874241658240647E-5
10	0.0	0.0	0.0	0.00977549924165824	1.9748483316481293E-4
11	0.0	0.0	0.0	0.011651605156723964	1.9748483316481293E-4
12	0.0	0.0	0.0	0.014515135237613752	1.9748483316481293E-4
13	0.0	0.0	0.0	0.01648998356926188	1.9748483316481293E-4
14	0.0	0.0	0.0	0.0198472257330637	3.9496966632962587E-4
15	0.0	0.0	0.0	0.026265482810920122	3.9496966632962587E-4
16	0.0	0.0	0.0	0.04147181496461072	3.9496966632962587E-4
17	0.0	0.0	0.0	0.06201023761375126	3.9496966632962587E-4
18	0.0	0.0	0.0	0.07188447927199192	3.9496966632962587E-4
19	0.0	0.0	0.0	0.08452350859453994	3.9496966632962587E-4
20	0.0	0.0	0.0	0.09508894716885744	3.9496966632962587E-4
21	0.0	0.0	0.0	0.12086071789686552	3.9496966632962587E-4
22	0.0	0.0	0.0	0.16963947168857432	3.9496966632962587E-4
23	0.0	0.0	0.0	0.2488308897876643	3.9496966632962587E-4
24	0.0	0.0	0.0	0.3768010616784631	3.9496966632962587E-4
25	0.0	0.0	0.0	0.4023753475733064	3.9496966632962587E-4
26	0.0	0.0	0.0	0.4529314648634985	3.9496966632962587E-4
27	0.0	0.0	0.0	0.48472652300303337	3.9496966632962587E-4
28	0.0	0.0	0.0	0.5321228829625885	4.937120829120324E-4
29	0.0	0.0	0.0	0.5987740141557129	5.924544994944388E-4
30	0.0	0.0	0.0	0.7225970045500506	5.924544994944388E-4
31	0.0	0.0	0.0	1.1513365773508595	5.924544994944388E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTTATAG	20	0.0018418323	37.0	36
CTTAGAA	35	2.3880297E-5	31.714287	2
CTCTATA	30	3.599179E-4	30.833334	2
CGTTATA	40	5.9394417E-5	27.750002	2
TTAGAAC	35	8.868663E-4	26.428572	3
ATCCGAC	35	8.868663E-4	26.428572	27
GTCGTAG	35	8.868663E-4	26.428572	13
TATAATC	45	1.3230607E-4	24.666666	3
TGCGGAC	40	0.0019310291	23.125002	13
TCATGGA	40	0.0019310291	23.125002	2
GGTATGC	40	0.0019310291	23.125002	30
CCTTGCT	105	4.0017767E-11	22.904764	15
CACTGCG	50	2.7017953E-4	22.199999	9
ACTCGCA	85	5.1792085E-8	21.764706	21
AGTCCTT	120	1.0913936E-11	21.583334	12
AATCCGA	45	0.0038254994	20.555555	26
TCCGTAT	90	9.4754796E-8	20.555555	13
GGACCGC	45	0.0038254994	20.555555	6
TATACGA	45	0.0038254994	20.555555	36
ACTTAGA	55	5.1425135E-4	20.181818	1
>>END_MODULE