FastQCFastQC Report
Fri 10 Feb 2017
ERR1630496.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630496.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences225486
Sequences flagged as poor quality0
Sequence length43
%GC32

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT127195.640704966161978No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT110264.8898822986792965No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT89433.966099890902318No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT55192.4476020684210993No Hit
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA18770.8324241859805043No Hit
GTACATGGGAATGGTATCAACGCAAAAAAAAAAAAAAAAAAAA14770.6550295805504555No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14290.6337422278988496No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT13920.6173332268965701No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12230.5423840061023745No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10550.46787827182175384No Hit
GTACATGGGAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAA9500.4213121878963661No Hit
GAATGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA9100.4035727273533612No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG7980.3539022378329475No Hit
CTTATACACATCTCCGAGCCCACGAGACACTCGCTAATCTCGT7880.3494673726971963No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7450.330397452613466No Hit
GTACATGGGAAGCAGAGGTATCAACGCAAAAAAAAAAAAAAAA7180.31842331674693775No Hit
TCTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTT6020.26697888117222357Illumina PCR Primer Index 10 (95% over 21bp)
TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5710.25323079925139474No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTT5460.2421436364120167No Hit
GTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAA5390.23903923081699088No Hit
CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5050.2239606893554367No Hit
ACGCAGAGTACATGGGAATGGTATCAACGCAAAAAAAAAAAAA5050.2239606893554367No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGT4990.22129977027398598No Hit
GAGTACATGGGAATGGTATCAACGCAAAAAAAAAAAAAAAAAA4700.2084386613803074No Hit
GTACATGGGAACATGGTATCAACGCAAAAAAAAAAAAAAAAAA4540.20134287716310548No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG4470.1982384715680796No Hit
GAGTACATGGGAACAGTGGTATCAACGCAAAAAAAAAAAAAAA4440.19690801202735425No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATATGCCCA4390.19469057945947862No Hit
GTACATGGGAAGCATGGTATCAACGCAAAAAAAAAAAAAAAAA4330.1920296603780279No Hit
CCACTGTTCCCATGTACTCTGCGTTGATACCCTGTCTCTTATA3700.16409001002279522No Hit
GTACATGGGAAGAGTGGTATCAACGCAAAAAAAAAAAAAAAAA3680.16320303699564495No Hit
GTACATGGGAATGTATCAACGCAAAAAAAAAAAAAAAAAAAAA3620.16054211791419423No Hit
ACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAAAAAAAAA3600.159655144887044No Hit
GGTATCAACGCAGAGTACATGGGAACAGTGGCTGTCTCTTATA3600.159655144887044No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGTT3580.15876817185989373No Hit
ACACATCTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCC3530.15655073929201813No Hit
GTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3530.15655073929201813No Hit
GTACATGGGAAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAA3440.15255936066984202No Hit
GAAGAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA3420.15167238764269178No Hit
GTACATGGGAAGATGGTATCAACGCAAAAAAAAAAAAAAAAAA3370.14945495507481615No Hit
TATCAACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAAAA3350.14856798204766591No Hit
GGTATCAACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAA3350.14856798204766591No Hit
ATACACATCTCCGAGCCCACGAGACACTCGCTAATCTCGTATG3340.1481244955340908No Hit
GAAGCAGAGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAA3230.14324614388476448No Hit
GAACATGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAA2980.13215898104538643No Hit
GAACAGTGGTATCAACGCAGAAAAAAAAAAAAAAAAAAAAAAA2840.1259501698553347No Hit
CATCTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTC2840.1259501698553347No Hit
TATCAACGCAGAGTACATGGGAATGGTATCAACGCAAAAAAAA2830.12550668334175957No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC2770.12284576426030884No Hit
ACGCAGAGTACATGGGAAGCAGAGGTATCAACGCAAAAAAAAA2670.11841089912455761No Hit
GAGTACATGGGAAGCAGAGGTATCAACGCAAAAAAAAAAAAAA2670.11841089912455761No Hit
GTACATGGGAACTGGTATCAACGCAAAAAAAAAAAAAAAAAAA2650.11752392609740737No Hit
CAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA2630.11663695307025713No Hit
GAATGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2590.11486300701595664No Hit
CCCATGTACTCTGCGTTGATACCCTGTCTCTTATACACATCTC2570.1139760339888064No Hit
ACGCAGAGTACATGGGAACAGTGGTATCAACGCAAAAAAAAAA2560.11353254747523127No Hit
GGTATCAACGCAGAGTACATGGGAATGGTATCAACGCAAAAAA2480.1099846553666303No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTG2450.10865419582590492No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAA2420.10732373628517956No Hit
CATGGGAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAA2310.10244538463585323No Hit
GTATCAACGCAGAGTACATGGGAATGGTATCAACGCAAAAAAA2280.10111492509512787No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTTAT200.001838799837.01
CTGTTTA200.001838799837.017
ATGCCCC251.2304803E-437.037
AGTGGCT803.274181E-1127.7527
CCGTCTT950.027.26315937
GTGGCTG901.4188117E-1024.66666628
CAGTGGC751.3613317E-824.66666626
GCCGTCT1103.6379788E-1223.54545436
TCTTTGC652.666995E-622.769238
TGGGCAG2700.021.9259266
GGCAGTC2350.021.2553188
CTTTGCG705.071968E-621.1428579
GGGCAGT2550.021.0392157
TATGCCG1151.3278623E-1020.91304433
TGCCGTC1151.3278623E-1020.91304435
TCCTCCG806.909195E-720.812521
TTAAAAC450.003816418320.55555536
AGTCGGT2250.020.55555511
CAGTCGG2400.020.04166610
ATGCCGT1202.3464963E-1020.04166634
Produced by FastQC (version 0.11.5)