##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1630496.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	225486
Sequences flagged as poor quality	0
Sequence length	43
%GC	32
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.85308622264797	33.0	31.0	34.0	30.0	34.0
2	31.84787525611346	33.0	31.0	34.0	30.0	34.0
3	31.809584630531386	33.0	31.0	34.0	28.0	34.0
4	35.558487001410285	37.0	35.0	37.0	33.0	37.0
5	35.603731495525224	37.0	35.0	37.0	33.0	37.0
6	35.78434581304383	37.0	35.0	37.0	35.0	37.0
7	35.6730218284062	37.0	35.0	37.0	33.0	37.0
8	35.80873757129046	37.0	35.0	37.0	35.0	37.0
9	37.28483364820876	39.0	37.0	39.0	34.0	39.0
10	37.26651765519811	39.0	37.0	39.0	34.0	39.0
11	37.43725552805939	39.0	37.0	39.0	35.0	39.0
12	37.4298759124735	39.0	37.0	39.0	35.0	39.0
13	37.47515145064438	39.0	37.0	39.0	35.0	39.0
14	38.372408930044436	40.0	38.0	41.0	34.0	41.0
15	38.278048304551056	40.0	38.0	41.0	34.0	41.0
16	38.11446386915374	40.0	37.0	41.0	33.0	41.0
17	38.451092307282934	40.0	38.0	41.0	34.0	41.0
18	38.6578013712603	40.0	38.0	41.0	35.0	41.0
19	38.85055391465546	40.0	38.0	41.0	35.0	41.0
20	38.896499117461836	40.0	38.0	41.0	35.0	41.0
21	38.86380529168108	40.0	38.0	41.0	35.0	41.0
22	38.69245540743106	40.0	38.0	41.0	35.0	41.0
23	38.54510701329573	40.0	38.0	41.0	35.0	41.0
24	38.413134296586044	40.0	38.0	41.0	34.0	41.0
25	38.31828583592773	40.0	37.0	41.0	34.0	41.0
26	37.92703759878662	40.0	36.0	41.0	34.0	41.0
27	37.618770123200555	40.0	35.0	41.0	33.0	41.0
28	37.37936723344243	39.0	35.0	41.0	33.0	41.0
29	37.05242010590458	39.0	35.0	41.0	33.0	41.0
30	36.63056686446165	38.0	35.0	40.0	32.0	41.0
31	36.09651153508422	37.0	35.0	40.0	31.0	41.0
32	35.610020134287716	37.0	35.0	40.0	31.0	41.0
33	35.166169961771466	35.0	35.0	40.0	30.0	41.0
34	34.808134429632	35.0	35.0	40.0	28.0	41.0
35	34.38187293224413	35.0	35.0	40.0	24.0	41.0
36	33.89843271866103	35.0	34.0	40.0	21.0	41.0
37	33.272136629325104	35.0	33.0	40.0	18.0	41.0
38	32.6647729792537	35.0	33.0	39.0	15.0	41.0
39	32.08516271520183	35.0	33.0	39.0	10.0	41.0
40	31.343134385283342	35.0	31.0	39.0	8.0	41.0
41	30.648913901528253	35.0	30.0	39.0	7.0	41.0
42	29.871029686987217	35.0	24.0	38.0	7.0	41.0
43	28.989649024773158	35.0	21.0	38.0	7.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	4.0
10	5.0
11	8.0
12	2.0
13	2.0
14	2.0
15	2.0
16	2.0
17	10.0
18	13.0
19	53.0
20	89.0
21	166.0
22	301.0
23	544.0
24	809.0
25	1407.0
26	2187.0
27	3167.0
28	4584.0
29	5913.0
30	7258.0
31	8157.0
32	9617.0
33	11497.0
34	14175.0
35	16885.0
36	23459.0
37	45068.0
38	39841.0
39	30258.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	55.037119821186245	17.802879114446128	15.198282820219436	11.961718244148194
2	15.560611301810312	29.57389815775702	33.34530746919987	21.520183071232804
3	23.85691351126012	34.34270863823031	31.145170875353678	10.655206975155886
4	11.685426146190894	17.705312081459603	27.679323771764103	42.9299380005854
5	8.899443867911977	49.67980273719876	27.041590165243072	14.379163229646185
6	27.44560637911001	29.306475790071225	21.069157286926902	22.17876054389186
7	20.858944679492296	39.639268069858	22.586324649867397	16.91546260078231
8	38.10480473288807	26.576372812502775	19.917866297685887	15.400956156923268
9	30.78993817798001	8.82804253922638	24.326122242622603	36.05589704017101
10	20.172871042991584	36.20712594130012	23.433827377309456	20.186175638398836
11	23.600134819900127	29.727344491454016	18.04014439920882	28.63237628943704
12	19.623391252672008	38.20192827936103	28.982287148647814	13.192393319319159
13	31.97981249390206	20.69574164249665	32.12261515127325	15.201830712328038
14	24.374905759115865	24.351844460409957	36.52954063666924	14.743709143804937
15	27.42831040508058	24.657406668263217	34.33117798887736	13.583104937778842
16	16.0652989542588	25.787853791366206	38.01388999760517	20.13295725676982
17	13.662932510222364	32.611337289233035	36.57566323408105	17.15006696646355
18	12.202974907533063	22.638212571955687	43.59782869002954	21.560983830481714
19	13.6123750476748	28.592462503215277	46.95280416522534	10.842358283884588
20	15.428452320764926	23.221840823820546	48.792829710048515	12.55687714536601
21	19.624278225699157	23.662222931800645	44.50342815074993	12.210070691750264
22	17.5092910424594	25.567440994119366	42.76540450404903	14.1578634593722
23	14.857685177793744	27.68686304249488	43.17208163699742	14.283370142713961
24	12.116938523899488	30.327825230834733	44.61296931960299	12.942266925662791
25	12.156408823607673	29.251926948901485	44.35308622264797	14.238578004842871
26	14.223055976867743	28.853232573197452	43.997853525274294	12.92585792466051
27	11.72046158076333	31.531891115191186	43.780988620136064	12.96665868390942
28	11.756827474876488	31.07820441180384	45.84896623293685	11.316001880382817
29	11.32487161065432	32.46631719929397	45.448941397692096	10.759869792359614
30	10.504865047053919	33.5905555112069	45.13539643259448	10.769183009144692
31	10.034769342664289	34.39193564123715	43.86835546331036	11.7049395527882
32	8.75176285889146	35.48468641068625	44.3827998190575	11.380750911364785
33	9.417879602281294	35.35474486220874	44.58281223667988	10.644563298830082
34	9.961150581410818	36.198699697542196	41.70768916917236	12.132460551874617
35	9.22185856328109	36.59428966765121	42.17689790053484	12.006953868532857
36	9.197023318520884	37.15175221521513	40.2685754326211	13.382649033642888
37	9.276407404450831	37.52383740010467	40.65440869943145	12.545346496013057
38	9.636961939987405	36.22087402322096	40.58877269542233	13.55339134136931
39	9.994855556442529	36.757049218133275	40.849099278890925	12.398995946533265
40	9.315434217645441	37.08744667074674	40.769271706447405	12.827847405160409
41	8.990358603194878	38.55228262508537	39.28713977807935	13.170218993640404
42	9.087038663154253	37.847582555014505	39.56919720071313	13.496181581118119
43	8.484340491205662	38.94122029749075	38.26224244520724	14.312196766096344
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	13.0
1	79.5
2	146.0
3	826.5
4	1507.0
5	1507.0
6	2310.5
7	3114.0
8	3168.5
9	3223.0
10	4200.5
11	5178.0
12	5178.0
13	9307.0
14	13436.0
15	20173.5
16	26911.0
17	23110.5
18	19310.0
19	19310.0
20	20098.0
21	20886.0
22	14213.0
23	7540.0
24	7393.5
25	7247.0
26	7247.0
27	7364.0
28	7481.0
29	7431.0
30	7381.0
31	6957.0
32	6533.0
33	6533.0
34	6160.0
35	5787.0
36	5560.5
37	5334.0
38	5770.5
39	6207.0
40	6207.0
41	7070.5
42	7934.0
43	8326.0
44	8718.0
45	10429.5
46	12141.0
47	12141.0
48	12828.0
49	13515.0
50	11996.5
51	10478.0
52	7585.0
53	4692.0
54	4692.0
55	4543.5
56	4395.0
57	4017.5
58	3640.0
59	3217.0
60	2794.0
61	2794.0
62	2599.0
63	2404.0
64	2258.0
65	2112.0
66	1911.0
67	1710.0
68	1710.0
69	1509.0
70	1308.0
71	1139.0
72	970.0
73	808.5
74	647.0
75	647.0
76	508.5
77	370.0
78	299.0
79	228.0
80	166.0
81	104.0
82	104.0
83	77.5
84	51.0
85	36.5
86	22.0
87	17.0
88	12.0
89	12.0
90	7.5
91	3.0
92	3.0
93	3.0
94	2.0
95	1.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	225486.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	33.88281312365291
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.31701155744034	27.213662932510225
2	9.52867109069253	6.457163637653779
3	3.2172353765003074	3.2702695511029507
4	1.6583552571301423	2.2475896507987194
5	0.9777358935092472	1.6564221282030813
6	0.7355924660671981	1.495436523775312
7	0.48821350505883426	1.157943286944644
8	0.35601628250939127	0.9650266535394658
9	0.28140992918940855	0.8581464037678614
>10	2.009136006073219	13.287742919737811
>50	0.22774571013468412	5.269950240813177
>100	0.17408149107995968	11.801176126234001
>500	0.01570660069894373	3.6263892215037745
>1k	0.007853300349471865	3.7487914992505074
>5k	0.0026177667831572887	6.413701959323418
>10k+	0.0026177667831572887	10.530587264841277
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	12719	5.640704966161978	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	11026	4.8898822986792965	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	8943	3.966099890902318	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5519	2.4476020684210993	No Hit
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA	1877	0.8324241859805043	No Hit
GTACATGGGAATGGTATCAACGCAAAAAAAAAAAAAAAAAAAA	1477	0.6550295805504555	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1429	0.6337422278988496	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1392	0.6173332268965701	No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1223	0.5423840061023745	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1055	0.46787827182175384	No Hit
GTACATGGGAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAA	950	0.4213121878963661	No Hit
GAATGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA	910	0.4035727273533612	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG	798	0.3539022378329475	No Hit
CTTATACACATCTCCGAGCCCACGAGACACTCGCTAATCTCGT	788	0.3494673726971963	No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	745	0.330397452613466	No Hit
GTACATGGGAAGCAGAGGTATCAACGCAAAAAAAAAAAAAAAA	718	0.31842331674693775	No Hit
TCTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTT	602	0.26697888117222357	Illumina PCR Primer Index 10 (95% over 21bp)
TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	571	0.25323079925139474	No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTT	546	0.2421436364120167	No Hit
GTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAA	539	0.23903923081699088	No Hit
CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	505	0.2239606893554367	No Hit
ACGCAGAGTACATGGGAATGGTATCAACGCAAAAAAAAAAAAA	505	0.2239606893554367	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGT	499	0.22129977027398598	No Hit
GAGTACATGGGAATGGTATCAACGCAAAAAAAAAAAAAAAAAA	470	0.2084386613803074	No Hit
GTACATGGGAACATGGTATCAACGCAAAAAAAAAAAAAAAAAA	454	0.20134287716310548	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG	447	0.1982384715680796	No Hit
GAGTACATGGGAACAGTGGTATCAACGCAAAAAAAAAAAAAAA	444	0.19690801202735425	No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATATGCCCA	439	0.19469057945947862	No Hit
GTACATGGGAAGCATGGTATCAACGCAAAAAAAAAAAAAAAAA	433	0.1920296603780279	No Hit
CCACTGTTCCCATGTACTCTGCGTTGATACCCTGTCTCTTATA	370	0.16409001002279522	No Hit
GTACATGGGAAGAGTGGTATCAACGCAAAAAAAAAAAAAAAAA	368	0.16320303699564495	No Hit
GTACATGGGAATGTATCAACGCAAAAAAAAAAAAAAAAAAAAA	362	0.16054211791419423	No Hit
ACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAAAAAAAAA	360	0.159655144887044	No Hit
GGTATCAACGCAGAGTACATGGGAACAGTGGCTGTCTCTTATA	360	0.159655144887044	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGTT	358	0.15876817185989373	No Hit
ACACATCTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCC	353	0.15655073929201813	No Hit
GTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	353	0.15655073929201813	No Hit
GTACATGGGAAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAA	344	0.15255936066984202	No Hit
GAAGAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA	342	0.15167238764269178	No Hit
GTACATGGGAAGATGGTATCAACGCAAAAAAAAAAAAAAAAAA	337	0.14945495507481615	No Hit
TATCAACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAAAA	335	0.14856798204766591	No Hit
GGTATCAACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAA	335	0.14856798204766591	No Hit
ATACACATCTCCGAGCCCACGAGACACTCGCTAATCTCGTATG	334	0.1481244955340908	No Hit
GAAGCAGAGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAA	323	0.14324614388476448	No Hit
GAACATGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAA	298	0.13215898104538643	No Hit
GAACAGTGGTATCAACGCAGAAAAAAAAAAAAAAAAAAAAAAA	284	0.1259501698553347	No Hit
CATCTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTC	284	0.1259501698553347	No Hit
TATCAACGCAGAGTACATGGGAATGGTATCAACGCAAAAAAAA	283	0.12550668334175957	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC	277	0.12284576426030884	No Hit
ACGCAGAGTACATGGGAAGCAGAGGTATCAACGCAAAAAAAAA	267	0.11841089912455761	No Hit
GAGTACATGGGAAGCAGAGGTATCAACGCAAAAAAAAAAAAAA	267	0.11841089912455761	No Hit
GTACATGGGAACTGGTATCAACGCAAAAAAAAAAAAAAAAAAA	265	0.11752392609740737	No Hit
CAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA	263	0.11663695307025713	No Hit
GAATGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	259	0.11486300701595664	No Hit
CCCATGTACTCTGCGTTGATACCCTGTCTCTTATACACATCTC	257	0.1139760339888064	No Hit
ACGCAGAGTACATGGGAACAGTGGTATCAACGCAAAAAAAAAA	256	0.11353254747523127	No Hit
GGTATCAACGCAGAGTACATGGGAATGGTATCAACGCAAAAAA	248	0.1099846553666303	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTG	245	0.10865419582590492	No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAA	242	0.10732373628517956	No Hit
CATGGGAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAA	231	0.10244538463585323	No Hit
GTATCAACGCAGAGTACATGGGAATGGTATCAACGCAAAAAAA	228	0.10111492509512787	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0017739460543004888	0.0
3	0.0	0.0	0.0	0.0022174325678756108	0.0
4	0.0	0.0	0.0	0.0039913786221761	0.0
5	0.0	0.0	0.0	0.004878351649326344	0.0
6	0.0	0.0	0.0	0.004878351649326344	0.0
7	0.0	0.0	0.0	0.00620881119005171	0.0
8	0.0	0.0	0.0	0.006652297703626833	0.0
9	0.0	0.0	0.0	0.009313216785077566	0.0
10	4.434865135751222E-4	0.0	0.0	0.01640900100227952	0.0
11	8.869730271502444E-4	0.0	0.0	0.021730839165180985	0.0
12	8.869730271502444E-4	0.0	0.0	0.02882662338238294	0.0
13	8.869730271502444E-4	0.0	0.0	0.0319310289774088	0.0
14	8.869730271502444E-4	4.434865135751222E-4	0.0	0.03947029970818587	0.0
15	8.869730271502444E-4	8.869730271502444E-4	0.0	0.051887922088289296	0.0
16	8.869730271502444E-4	8.869730271502444E-4	0.0	0.09135822179647517	0.0
17	8.869730271502444E-4	8.869730271502444E-4	0.0	0.1321589810453864	0.0
18	8.869730271502444E-4	8.869730271502444E-4	0.0	0.15655073929201813	0.0
19	8.869730271502444E-4	8.869730271502444E-4	0.0	0.18936874129657716	0.0
20	8.869730271502444E-4	8.869730271502444E-4	0.0	0.2181953646789601	0.0
21	8.869730271502444E-4	8.869730271502444E-4	0.0	0.29314458547315575	0.0
22	8.869730271502444E-4	8.869730271502444E-4	0.0	0.42929494514071825	0.0
23	8.869730271502444E-4	8.869730271502444E-4	0.0	0.6652297703626833	0.0
24	8.869730271502444E-4	8.869730271502444E-4	0.0	0.9681310591344917	0.0
25	8.869730271502444E-4	8.869730271502444E-4	0.0	1.0315496305757341	0.0
26	8.869730271502444E-4	8.869730271502444E-4	0.0	1.150847502727442	0.0
27	8.869730271502444E-4	8.869730271502444E-4	0.0	1.2284576426030884	0.0
28	8.869730271502444E-4	8.869730271502444E-4	0.0	1.3011894308294085	0.0
29	8.869730271502444E-4	0.0013304595407253666	0.0	1.4235917085761423	0.0
30	8.869730271502444E-4	0.0013304595407253666	0.0	1.6759355348003868	0.0
31	8.869730271502444E-4	0.0013304595407253666	0.0	2.3748702801947794	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCGTTAT	20	0.0018387998	37.0	1
CTGTTTA	20	0.0018387998	37.0	17
ATGCCCC	25	1.2304803E-4	37.0	37
AGTGGCT	80	3.274181E-11	27.75	27
CCGTCTT	95	0.0	27.263159	37
GTGGCTG	90	1.4188117E-10	24.666666	28
CAGTGGC	75	1.3613317E-8	24.666666	26
GCCGTCT	110	3.6379788E-12	23.545454	36
TCTTTGC	65	2.666995E-6	22.76923	8
TGGGCAG	270	0.0	21.925926	6
GGCAGTC	235	0.0	21.255318	8
CTTTGCG	70	5.071968E-6	21.142857	9
GGGCAGT	255	0.0	21.039215	7
TATGCCG	115	1.3278623E-10	20.913044	33
TGCCGTC	115	1.3278623E-10	20.913044	35
TCCTCCG	80	6.909195E-7	20.8125	21
TTAAAAC	45	0.0038164183	20.555555	36
AGTCGGT	225	0.0	20.555555	11
CAGTCGG	240	0.0	20.041666	10
ATGCCGT	120	2.3464963E-10	20.041666	34
>>END_MODULE