FastQCFastQC Report
Fri 10 Feb 2017
ERR1630495.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630495.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences597764
Sequences flagged as poor quality0
Sequence length43
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT109391.8299864160437898No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT94411.5793858445808044No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT81421.3620760032387362No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT70211.1745437998942727No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20420.34160638646689995No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17170.28723710360610544No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT16680.2790398886517087No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA16550.2768651173372769No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13910.2327005306442007No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC13270.22199396417315193No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTT12320.20610140456768894No Hit
TGCCTACCCTACGCCGGCAGGCGCGGGTAACCCGTTGAACCCC11710.19589670839997053No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATG10670.1784985378845163No Hit
CTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCGAGATTGAGC10580.17699292697452507No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9770.16344242878460397No Hit
GGGTTCAACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGGCAC9640.16126765747017216No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG8870.14838631968469163No Hit
TCTTAGAGGGACAAGTGGCGTTCAGCCACCCGAGATTGAGCAA8730.1460442582691497No Hit
GCGCTACACTGACTGGCTCAGCGTGTGCCTACCCTACGCCGGC8720.14587696816803958No Hit
AGTCAGTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATC8170.13667601260698203No Hit
TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8020.13416666109032996No Hit
ACCCGAGATTGAGCAATAACAGGTCTGTGATGCCCTTAGATGT7460.12479841542816228No Hit
CATCTAAGGGCATCACAGACCTGTTATTGCTCAATCTCGGGTG7280.12178719360817981No Hit
GGTCTGTGATGCCCTTAGATGTCCGGGGCTGCACGCGCGCTAC7170.11994700249596829No Hit
GCCCCGGACATCTAAGGGCATCACAGACCTGTTATTGCTCAAT7050.11793952128264668No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6940.11609933017043515No Hit
TCACAGACCTGTTATTGCTCAATCTCGGGTGGCTGAACGCCAC6850.11459371926044393No Hit
ACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATC6840.11442642915933379No Hit
CCTTAGATGTCCGGGGCTGCACGCGCGCTACACTGACTGGCTC6820.11409184895711351No Hit
ACACTGACTGGCTCAGCGTGTGCCTACCCTACGCCGGCAGGCG6520.10907314592380939No Hit
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA6300.10539276369938638No Hit
CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6060.1013778012727431No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCGTCT706.5792847E-926.42857236
AGCGTCA400.001930109223.1253
GCGTAAC400.001930109223.12511
TGGGTAG450.00382369220.5555556
TGCCGAG450.00382369220.55555515
GGCGGTA851.2432629E-619.58823625
TTCTGCG851.2432629E-619.5882367
AATACTG851.2432629E-619.5882365
TTAACGG1052.2539098E-819.38095335
GATCGTT609.2297455E-418.511
TAACGGC1103.8424332E-818.536
ACTGTCC801.6145565E-518.58
CGGTACA902.1487012E-618.527
GTAACTA609.2297455E-418.513
ATATATG500.007031030518.4999982
CGTAACT500.007031030518.49999812
CTCGAAT500.007031030518.49999833
CGGTCTA500.007031030518.49999820
CGCGTAA500.007031030518.49999810
TCGCGTA500.007031030518.4999989
Produced by FastQC (version 0.11.5)