##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1630495.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	597764
Sequences flagged as poor quality	0
Sequence length	43
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.02959696468841	33.0	31.0	34.0	30.0	34.0
2	32.13647693738666	33.0	31.0	34.0	30.0	34.0
3	32.195021781171164	34.0	31.0	34.0	30.0	34.0
4	35.79565681439498	37.0	35.0	37.0	35.0	37.0
5	35.7903102227635	37.0	35.0	37.0	35.0	37.0
6	35.91767319544168	37.0	35.0	37.0	35.0	37.0
7	35.85508494991334	37.0	35.0	37.0	35.0	37.0
8	35.9100062901078	37.0	35.0	37.0	35.0	37.0
9	37.52570077823355	39.0	37.0	39.0	35.0	39.0
10	37.48280257760588	39.0	37.0	39.0	35.0	39.0
11	37.602697720170504	39.0	37.0	39.0	35.0	39.0
12	37.5834995081671	39.0	37.0	39.0	35.0	39.0
13	37.61408515735307	39.0	37.0	39.0	35.0	39.0
14	38.819743243152814	40.0	38.0	41.0	35.0	41.0
15	38.800073273064285	40.0	38.0	41.0	35.0	41.0
16	38.70003379260042	40.0	38.0	41.0	34.0	41.0
17	38.79137084200454	40.0	38.0	41.0	35.0	41.0
18	38.80809483341252	40.0	38.0	41.0	35.0	41.0
19	38.90999290689971	40.0	38.0	41.0	35.0	41.0
20	38.88233985318621	40.0	38.0	41.0	35.0	41.0
21	38.83457183771522	40.0	38.0	41.0	35.0	41.0
22	38.70226376964822	40.0	38.0	41.0	35.0	41.0
23	38.582510823669544	40.0	38.0	41.0	34.0	41.0
24	38.49299890926854	40.0	38.0	41.0	34.0	41.0
25	38.40748020958104	40.0	38.0	41.0	34.0	41.0
26	38.15618371129744	40.0	37.0	41.0	34.0	41.0
27	37.93727290368774	40.0	37.0	41.0	33.0	41.0
28	37.7442435476208	40.0	36.0	41.0	33.0	41.0
29	37.60596489584518	40.0	36.0	41.0	33.0	41.0
30	37.381019934288446	39.0	35.0	41.0	33.0	41.0
31	37.19659598102261	39.0	35.0	41.0	33.0	41.0
32	36.95134534699313	39.0	35.0	41.0	32.0	41.0
33	36.69006497547527	39.0	35.0	41.0	31.0	41.0
34	36.50396644829732	39.0	35.0	41.0	31.0	41.0
35	36.264572640707705	39.0	35.0	41.0	30.0	41.0
36	36.08056356689262	39.0	35.0	41.0	30.0	41.0
37	35.75589363026211	38.0	35.0	41.0	29.0	41.0
38	35.415632256208134	38.0	35.0	41.0	26.0	41.0
39	35.12212511961242	38.0	35.0	40.0	24.0	41.0
40	34.70974665587088	38.0	35.0	40.0	22.0	41.0
41	34.392623175701445	38.0	34.0	40.0	20.0	41.0
42	33.976527525913234	38.0	33.0	40.0	16.0	41.0
43	33.15708373204141	37.0	33.0	40.0	12.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	2.0
9	2.0
10	5.0
11	7.0
12	1.0
13	4.0
14	4.0
15	5.0
16	13.0
17	16.0
18	32.0
19	89.0
20	161.0
21	286.0
22	500.0
23	879.0
24	1498.0
25	2274.0
26	3471.0
27	5107.0
28	7297.0
29	9771.0
30	12688.0
31	15272.0
32	18823.0
33	23754.0
34	30292.0
35	38310.0
36	52934.0
37	88615.0
38	121533.0
39	164119.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	47.35096124892098	20.5574440749192	12.90827149175929	19.183323184400532
2	18.81361206094713	22.96441404969185	33.35948635247355	24.862487536887468
3	23.972002328678208	26.826138743718257	28.056222857181094	21.14563607042244
4	13.722137833660106	16.60605188669776	32.3932187284614	37.27859155118073
5	12.131041682001593	42.03230706432639	31.31904898923321	14.517602264438809
6	31.866087619863357	32.20869774693692	17.65345520974833	18.271759423451396
7	26.092571650350305	34.19560227782202	21.746542113610055	17.965283958217622
8	30.90868637121004	29.766596850931137	20.219016200373392	19.10570057748543
9	28.04986583333891	12.384988055486781	23.30083444302434	36.26431166814997
10	20.84016434579533	28.73157299536272	29.409599775162103	21.01866288367985
11	31.361707965016294	23.717386794788577	20.306174343051772	24.614730897143357
12	21.695518632771464	30.408488968890733	27.711772538995323	20.184219859342484
13	29.066822357987434	20.62134889354327	29.97470573671215	20.33712301175715
14	22.356314532156503	23.454574045944554	29.50294765158156	24.686163770317382
15	26.798535877035086	26.04991267456722	27.896795390823137	19.25475605757456
16	22.478436305966902	26.066139814374907	30.218447414029615	21.236976465628576
17	21.29318593960158	28.048025642226698	28.85603683058866	21.80275158758306
18	19.9485081068783	23.639931477974585	33.1341465862782	23.277413828868916
19	20.21834703996895	26.48938377018355	33.683025408020555	19.60924378182694
20	21.44792928312846	23.574521048440523	35.08926599795237	19.88828367047865
21	22.80398284272723	25.517930153036982	32.79755890284461	18.880528101391185
22	23.420446865318084	24.656051552117557	32.43203003191895	19.491471550645407
23	21.27997002161388	25.159427466357958	33.16777189660133	20.392830615426824
24	19.184661505209412	27.332024009475308	32.83519917559438	20.648115309720893
25	19.959214673349347	26.508789421912326	33.42171827008652	20.1102776346518
26	19.98782128063918	28.031296632115684	32.49325820892526	19.48762387831987
27	20.748656660488084	26.91697726862106	32.9098105606895	19.42455551020135
28	18.982909643270588	27.262264037312384	33.7894219123266	19.96540440709042
29	18.987928346303892	27.51788331180867	34.181048039025434	19.313140302862
30	17.285584277407136	29.046078385449775	34.24445098734618	19.42388634979691
31	19.47541170093883	29.041896132922023	31.84584551762903	19.636846648510115
32	18.3609250473431	28.326229080372855	32.890572199061836	20.422273673222207
33	17.645927155198375	28.50656780936958	32.97689389123467	20.870611144197376
34	19.124938939113097	28.231542883144517	32.95514617809035	19.688371999652038
35	18.133075929631094	28.695605623624036	33.21561686551883	19.955701581226034
36	18.01831492026954	29.388855802624448	31.754170542220677	20.83865873488534
37	18.746194150199745	28.090350037807564	32.59731265181577	20.566143160176924
38	18.120696462148942	27.393921346886064	32.23931183544007	22.24607035552492
39	18.600986342436148	28.401342335771307	32.67359693792199	20.324074383870556
40	17.741282512831148	28.05655743738332	32.54444897986497	21.65771106992057
41	17.487336139345963	28.395989052535786	32.692333429246325	21.42434137887193
42	17.702303919272488	28.72287391010499	32.80809817921454	20.766723991407982
43	16.88944131797833	27.53494690212191	31.946052288194004	23.629559491705756
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1260.0
1	916.0
2	572.0
3	1244.0
4	1916.0
5	1916.0
6	2847.5
7	3779.0
8	3805.0
9	3831.0
10	5742.5
11	7654.0
12	7654.0
13	12892.0
14	18130.0
15	22303.0
16	26476.0
17	23900.0
18	21324.0
19	21324.0
20	22178.0
21	23032.0
22	17085.0
23	11138.0
24	10099.5
25	9061.0
26	9061.0
27	9466.0
28	9871.0
29	10566.5
30	11262.0
31	12432.5
32	13603.0
33	13603.0
34	15524.0
35	17445.0
36	18753.0
37	20061.0
38	23256.0
39	26451.0
40	26451.0
41	28804.0
42	31157.0
43	33480.5
44	35804.0
45	37236.0
46	38668.0
47	38668.0
48	40316.0
49	41964.0
50	39852.0
51	37740.0
52	37158.0
53	36576.0
54	36576.0
55	33542.5
56	30509.0
57	27318.5
58	24128.0
59	22711.5
60	21295.0
61	21295.0
62	20966.5
63	20638.0
64	18223.0
65	15808.0
66	15092.0
67	14376.0
68	14376.0
69	11537.0
70	8698.0
71	7420.0
72	6142.0
73	4399.5
74	2657.0
75	2657.0
76	2059.5
77	1462.0
78	1191.0
79	920.0
80	760.5
81	601.0
82	601.0
83	585.5
84	570.0
85	549.5
86	529.0
87	428.5
88	328.0
89	328.0
90	303.0
91	278.0
92	157.5
93	37.0
94	21.0
95	5.0
96	5.0
97	6.0
98	7.0
99	4.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	597764.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	40.16998515663027
#Duplication Level	Percentage of deduplicated	Percentage of total
1	82.82912962686059	33.272449076475944
2	8.174236821546776	6.567179435766292
3	2.558916122159269	3.0837486793259914
4	1.3317116369374196	2.139793467547517
5	0.8719443701067999	1.751299620229874
6	0.5796415313387269	1.3970515026025854
7	0.44048140157197996	1.2385891954042691
8	0.37029222499025527	1.189970654517932
9	0.2928370492772968	1.0586933930502376
>10	2.1122699299147554	16.85377251781854
>50	0.2564410677855409	7.1167433261968585
>100	0.16539195902337575	12.949020094563446
>500	0.010859068016686287	3.0147047508384155
>1k	0.0041765646218024175	2.4038449368363746
>5k	0.0012529693865407255	4.127875546197806
>10k+	4.176564621802418E-4	1.8352638026279386
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	10939	1.8299864160437898	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	9441	1.5793858445808044	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	8142	1.3620760032387362	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	7021	1.1745437998942727	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2042	0.34160638646689995	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1717	0.28723710360610544	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1668	0.2790398886517087	No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA	1655	0.2768651173372769	No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1391	0.2327005306442007	No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC	1327	0.22199396417315193	No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTT	1232	0.20610140456768894	No Hit
TGCCTACCCTACGCCGGCAGGCGCGGGTAACCCGTTGAACCCC	1171	0.19589670839997053	No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATG	1067	0.1784985378845163	No Hit
CTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCGAGATTGAGC	1058	0.17699292697452507	No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	977	0.16344242878460397	No Hit
GGGTTCAACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGGCAC	964	0.16126765747017216	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG	887	0.14838631968469163	No Hit
TCTTAGAGGGACAAGTGGCGTTCAGCCACCCGAGATTGAGCAA	873	0.1460442582691497	No Hit
GCGCTACACTGACTGGCTCAGCGTGTGCCTACCCTACGCCGGC	872	0.14587696816803958	No Hit
AGTCAGTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATC	817	0.13667601260698203	No Hit
TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	802	0.13416666109032996	No Hit
ACCCGAGATTGAGCAATAACAGGTCTGTGATGCCCTTAGATGT	746	0.12479841542816228	No Hit
CATCTAAGGGCATCACAGACCTGTTATTGCTCAATCTCGGGTG	728	0.12178719360817981	No Hit
GGTCTGTGATGCCCTTAGATGTCCGGGGCTGCACGCGCGCTAC	717	0.11994700249596829	No Hit
GCCCCGGACATCTAAGGGCATCACAGACCTGTTATTGCTCAAT	705	0.11793952128264668	No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	694	0.11609933017043515	No Hit
TCACAGACCTGTTATTGCTCAATCTCGGGTGGCTGAACGCCAC	685	0.11459371926044393	No Hit
ACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATC	684	0.11442642915933379	No Hit
CCTTAGATGTCCGGGGCTGCACGCGCGCTACACTGACTGGCTC	682	0.11409184895711351	No Hit
ACACTGACTGGCTCAGCGTGTGCCTACCCTACGCCGGCAGGCG	652	0.10907314592380939	No Hit
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA	630	0.10539276369938638	No Hit
CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	606	0.1013778012727431	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	6.691604044405485E-4	0.0
3	0.0	0.0	0.0	0.0010037406066608226	0.0
4	0.0	0.0	0.0	0.0023420614155419198	0.0
5	0.0	0.0	0.0	0.002676641617762194	0.0
6	0.0	0.0	0.0	0.002676641617762194	0.0
7	0.0	0.0	0.0	0.003011221819982468	0.0
8	0.0	0.0	0.0	0.003011221819982468	0.0
9	0.0	0.0	0.0	0.005520573336634525	0.0
10	0.0	1.6729010111013711E-4	0.0	0.00869908525772713	0.0
11	0.0	1.6729010111013711E-4	0.0	0.01271404768437042	0.0
12	0.0	1.6729010111013711E-4	0.0	0.01856920122322522	0.0
13	0.0	1.6729010111013711E-4	0.0	0.022249583447648234	0.0
14	0.0	1.6729010111013711E-4	0.0	0.02910847759316386	0.0
15	0.0	1.6729010111013711E-4	0.0	0.04232439558086469	0.0
16	0.0	1.6729010111013711E-4	0.0	0.06323565821963183	0.0
17	0.0	1.6729010111013711E-4	0.0	0.09803199925054035	0.0
18	0.0	1.6729010111013711E-4	0.0	0.1077348251149283	0.0
19	0.0	1.6729010111013711E-4	0.0	0.12964982836035627	0.0
20	0.0	1.6729010111013711E-4	0.0	0.14587696816803955	0.0
21	0.0	1.6729010111013711E-4	0.0	0.1820116300078292	0.0
22	0.0	1.6729010111013711E-4	0.0	0.24959683085632456	0.0
23	0.0	3.3458020222027423E-4	0.0	0.35314940344349943	0.0
24	0.0	3.3458020222027423E-4	0.0	0.5329862621368968	0.0
25	0.0	3.3458020222027423E-4	0.0	0.5769835587288629	0.0
26	0.0	3.3458020222027423E-4	0.0	0.6510930735206536	0.0
27	0.0	3.3458020222027423E-4	0.0	0.7183436941669288	0.0
28	0.0	3.3458020222027423E-4	0.0	0.8073420279575217	0.0
29	0.0	3.3458020222027423E-4	0.0	0.9448544910700544	0.0
30	0.0	3.3458020222027423E-4	0.0	1.1690232265576381	0.0
31	0.0	3.3458020222027423E-4	0.0	1.7550404507464483	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCCGTCT	70	6.5792847E-9	26.428572	36
AGCGTCA	40	0.0019301092	23.125	3
GCGTAAC	40	0.0019301092	23.125	11
TGGGTAG	45	0.003823692	20.555555	6
TGCCGAG	45	0.003823692	20.555555	15
GGCGGTA	85	1.2432629E-6	19.588236	25
TTCTGCG	85	1.2432629E-6	19.588236	7
AATACTG	85	1.2432629E-6	19.588236	5
TTAACGG	105	2.2539098E-8	19.380953	35
GATCGTT	60	9.2297455E-4	18.5	11
TAACGGC	110	3.8424332E-8	18.5	36
ACTGTCC	80	1.6145565E-5	18.5	8
CGGTACA	90	2.1487012E-6	18.5	27
GTAACTA	60	9.2297455E-4	18.5	13
ATATATG	50	0.0070310305	18.499998	2
CGTAACT	50	0.0070310305	18.499998	12
CTCGAAT	50	0.0070310305	18.499998	33
CGGTCTA	50	0.0070310305	18.499998	20
CGCGTAA	50	0.0070310305	18.499998	10
TCGCGTA	50	0.0070310305	18.499998	9
>>END_MODULE