FastQCFastQC Report
Fri 10 Feb 2017
ERR1630494.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630494.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1817504
Sequences flagged as poor quality0
Sequence length43
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT70970.3904805711569273No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT66390.3652811768227195No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT55730.30662931140729266No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT39930.21969690300544042No Hit
ACCTAGGCCTGGTCAGCATGTTGATGTATCTACGGAGATCAGC35880.19741359578850995No Hit
GGCCTAGGTATGGGAAAAGACACAAAGAGGACACGCTGGCCTT27560.1516365300984207No Hit
GCTTTATTGAGCCTGTGTGGGAGCAGGGAGCAAGCTTTGGCCA23210.12770260753208795No Hit
GTGTGGGAGCAGGGAGCAAGCTTTGGCCAGAGCCAAGGGTGCA22170.1219804743208268No Hit
GTATGCAGCTGATCTCCGTAGATACATCAACATGCTGACCAGG21440.11796397697061464No Hit
GGGTACACTGGCTCCAGTGGGGCTCCCTGGGCACCCAGCAGTG20160.11092135147983168No Hit
AGTGTACCCAGGGGACAATGCCACACCAGAGCAGATGGCCCAG19000.10453897212880962No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCTAA451.3236259E-424.66666614
TCTATAC1750.019.0285723
CGTTATA500.007036417318.52
CTAGCGG2500.017.76000229
TCTAGCG2550.017.41176428
CGAATTG1005.881573E-616.65000214
GCAGTCG2950.016.3050849
CGAACGA1251.6591548E-716.2816
CAGATCG803.383918E-416.18759
CGCAATA2750.016.14545436
TACCGTC1851.8189894E-1116.07
AGTCGGT3050.015.77049311
CTTATAC12750.015.5254937
CCGGTCC3250.015.3692319
CGGTCCA3250.015.36923110
GCTTTAT7350.015.3537421
ATACCGT1954.1836756E-1115.1794866
CTTGCGC3450.015.0144933
CGTCGTA1853.0559022E-1015.010
GGTATCA36550.014.830371
Produced by FastQC (version 0.11.5)