##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1630494.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1817504
Sequences flagged as poor quality	0
Sequence length	43
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.10943084581932	33.0	31.0	34.0	30.0	34.0
2	32.27502552951741	34.0	31.0	34.0	30.0	34.0
3	32.38546985316126	34.0	31.0	34.0	30.0	34.0
4	35.95475223163195	37.0	35.0	37.0	35.0	37.0
5	35.908770489638535	37.0	35.0	37.0	35.0	37.0
6	35.98428614187369	37.0	35.0	37.0	35.0	37.0
7	35.932169612831665	37.0	35.0	37.0	35.0	37.0
8	35.93478418754512	37.0	35.0	37.0	35.0	37.0
9	37.65249374966988	39.0	37.0	39.0	35.0	39.0
10	37.56719820148952	39.0	37.0	39.0	35.0	39.0
11	37.65828410831558	39.0	37.0	39.0	35.0	39.0
12	37.6077026515485	39.0	37.0	39.0	35.0	39.0
13	37.647555108544466	39.0	37.0	39.0	35.0	39.0
14	38.95197039456309	40.0	38.0	41.0	36.0	41.0
15	38.966954680704966	40.0	38.0	41.0	36.0	41.0
16	38.92849864429459	40.0	38.0	41.0	36.0	41.0
17	38.92335642727609	40.0	38.0	41.0	36.0	41.0
18	38.91246951863655	40.0	38.0	41.0	35.0	41.0
19	38.96516761448668	40.0	38.0	41.0	35.0	41.0
20	38.93830054844446	40.0	38.0	41.0	35.0	41.0
21	38.891333939292565	40.0	38.0	41.0	35.0	41.0
22	38.82686255436027	40.0	38.0	41.0	35.0	41.0
23	38.78010832438333	40.0	38.0	41.0	35.0	41.0
24	38.72569468898005	40.0	38.0	41.0	35.0	41.0
25	38.683270573269716	40.0	38.0	41.0	34.0	41.0
26	38.55796135799426	40.0	38.0	41.0	34.0	41.0
27	38.42923206771484	40.0	38.0	41.0	34.0	41.0
28	38.332444385266825	40.0	38.0	41.0	34.0	41.0
29	38.26961976424811	40.0	38.0	41.0	34.0	41.0
30	38.18392421694808	40.0	38.0	41.0	34.0	41.0
31	38.13784123721323	40.0	38.0	41.0	33.0	41.0
32	38.056500013204925	40.0	37.0	41.0	33.0	41.0
33	37.96907352060848	40.0	37.0	41.0	33.0	41.0
34	37.91074352518619	40.0	37.0	41.0	33.0	41.0
35	37.834498851171716	40.0	37.0	41.0	33.0	41.0
36	37.80408956458968	40.0	37.0	41.0	33.0	41.0
37	37.66857679542934	40.0	37.0	41.0	33.0	41.0
38	37.52319719791538	40.0	37.0	41.0	32.0	41.0
39	37.461527457436134	40.0	37.0	41.0	32.0	41.0
40	37.28226622885011	40.0	36.0	41.0	32.0	41.0
41	37.2263043162491	40.0	36.0	41.0	31.0	41.0
42	37.0800410893181	40.0	36.0	41.0	31.0	41.0
43	36.298158903639276	39.0	35.0	40.0	30.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	0.0
9	7.0
10	5.0
11	8.0
12	5.0
13	2.0
14	4.0
15	5.0
16	3.0
17	11.0
18	37.0
19	84.0
20	172.0
21	364.0
22	742.0
23	1200.0
24	2055.0
25	3581.0
26	5673.0
27	8457.0
28	12217.0
29	17620.0
30	23683.0
31	31227.0
32	40841.0
33	52955.0
34	70723.0
35	95292.0
36	135405.0
37	215430.0
38	403260.0
39	696435.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.04017597760445	18.93519904220293	11.565586650703382	24.459038329489232
2	18.885460499674277	20.503118562600136	34.45323916756167	26.158181770163917
3	20.72952796802648	23.580360758490766	28.691106044333324	26.999005229149425
4	13.978566209482896	14.620325457330493	34.70578331602021	36.6953250171664
5	14.053614187369051	37.55722133211261	34.47783333626776	13.911331144250576
6	35.725423437857636	34.60058409775164	15.3379029702273	14.336089494163422
7	29.487582970931562	30.07811812243604	21.651836804760816	18.782462101871577
8	28.590638590066376	32.175995211014666	19.744770850573094	19.488595348345864
9	26.945470271317145	13.170700036973784	19.86168943782242	40.022140253886654
10	17.030168846946143	26.8626644012888	32.90336637498459	23.203800376780464
11	35.67937126960931	21.723473510924872	21.6639413173231	20.933213902142718
12	22.89612567565188	25.58833433103861	28.612426712678484	22.90311328063102
13	30.531927302498374	19.65107091923869	25.472516154022216	24.344485624240715
14	22.917693716217403	20.626144426642252	25.840603376938926	30.615558480201422
15	25.9136706164058	27.978480377484725	22.66718532668979	23.44066367941969
16	25.224153564448827	26.30596686444707	24.448309329718118	24.02157024138599
17	23.97529799109108	26.28935066992975	25.431525872845395	24.303825466133773
18	23.337225117523815	24.957194041938834	27.713666654928957	23.991914185608394
19	24.542779548215574	24.970233903199112	27.51339199253482	22.973594556050493
20	25.147234889166683	24.43158309417751	26.616172509111397	23.805009507544412
21	25.11400250013205	24.84753816222688	26.579363786819727	23.459095550821345
22	24.968033082733243	24.908390848108176	26.722967322217723	23.40060874694086
23	24.287759476732926	24.914443104389317	26.49534746553515	24.302449953342606
24	23.776123738929872	25.579145905593602	26.856997288589184	23.787733066887334
25	24.203853196471645	25.386739176364948	26.41754846206666	23.99185916509675
26	24.06729228656443	25.855128792013666	26.568689807560258	23.50888911386165
27	24.363687782805428	24.915103350529076	26.518896244519958	24.202312622145534
28	23.64253393665158	25.481154374350755	26.866625878127365	24.009685810870295
29	23.782616159304187	25.875211278764727	27.161480800042252	23.180691761888834
30	23.778544641442327	25.882308924767155	26.423325615789565	23.915820818000952
31	23.213428966318645	26.437851030864305	26.731990686127787	23.616729316689263
32	23.080719492226702	25.545033188372624	26.60736922724792	24.766878092152755
33	22.6416558092857	26.135623360388756	27.017437100551085	24.20528372977446
34	24.56022105040759	24.845062239202775	26.698923358628097	23.895793351761537
35	23.581681250770288	25.200329682905785	26.96632304523126	24.251666021092664
36	22.80869808264521	26.16373884184024	26.988221208866666	24.039341866647888
37	23.700195432857367	24.746630543866754	27.539471715055374	24.013702308220505
38	23.57672940472208	24.97925726710918	27.10783580118668	24.33617752698206
39	23.347458932690106	24.838349736781872	27.47471257284716	24.33947875768086
40	22.618767276440657	24.700523355106785	27.969842157156187	24.71086721129637
41	21.742070443861472	25.102173090127998	28.60224791802384	24.553508547986688
42	22.51065197105481	25.01193945102734	28.788602390971352	23.688806186946493
43	22.668450798457666	24.395654700072186	28.383156240646514	24.552738260823634
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	205.0
1	248.5
2	292.0
3	904.0
4	1516.0
5	1516.0
6	2084.0
7	2652.0
8	2512.0
9	2372.0
10	3479.0
11	4586.0
12	4586.0
13	7509.5
14	10433.0
15	14241.5
16	18050.0
17	17241.0
18	16432.0
19	16432.0
20	19112.5
21	21793.0
22	21178.0
23	20563.0
24	23490.0
25	26417.0
26	26417.0
27	30240.0
28	34063.0
29	38790.0
30	43517.0
31	48995.0
32	54473.0
33	54473.0
34	60900.5
35	67328.0
36	73396.5
37	79465.0
38	86450.0
39	93435.0
40	93435.0
41	97164.5
42	100894.0
43	102610.0
44	104326.0
45	106547.5
46	108769.0
47	108769.0
48	114146.0
49	119523.0
50	118845.0
51	118167.0
52	117716.5
53	117266.0
54	117266.0
55	113493.5
56	109721.0
57	107292.5
58	104864.0
59	106680.0
60	108496.0
61	108496.0
62	99930.5
63	91365.0
64	83886.5
65	76408.0
66	66582.5
67	56757.0
68	56757.0
69	50133.0
70	43509.0
71	36743.5
72	29978.0
73	22006.5
74	14035.0
75	14035.0
76	10206.5
77	6378.0
78	5071.0
79	3764.0
80	3060.0
81	2356.0
82	2356.0
83	1826.5
84	1297.0
85	1099.0
86	901.0
87	815.5
88	730.0
89	730.0
90	553.0
91	376.0
92	199.5
93	23.0
94	15.5
95	8.0
96	8.0
97	4.0
98	0.0
99	0.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1817504.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	56.115924089884416
#Duplication Level	Percentage of deduplicated	Percentage of total
1	86.5093106236745	48.5454990802635
2	8.37402569324507	9.398323802577641
3	2.076972868972684	3.496537555560618
4	0.8549316102372201	1.91901109368458
5	0.45556447094461827	1.2782210634788276
6	0.2715127873542009	0.9141714578760762
7	0.18591877161498932	0.7303102572381909
8	0.15929348153508302	0.7151120734268901
9	0.12124164939825803	0.6123228474748525
>10	0.7753810265400538	8.67106580781023
>50	0.10084660268571861	3.970079921403809
>100	0.10004788871009496	11.556828824305063
>500	0.011706253193474686	4.403762447315416
>1k	0.002951156267262526	2.7228528074438474
>5k	2.951156267262526E-4	1.0659009601405625
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	7097	0.3904805711569273	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	6639	0.3652811768227195	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	5573	0.30662931140729266	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3993	0.21969690300544042	No Hit
ACCTAGGCCTGGTCAGCATGTTGATGTATCTACGGAGATCAGC	3588	0.19741359578850995	No Hit
GGCCTAGGTATGGGAAAAGACACAAAGAGGACACGCTGGCCTT	2756	0.1516365300984207	No Hit
GCTTTATTGAGCCTGTGTGGGAGCAGGGAGCAAGCTTTGGCCA	2321	0.12770260753208795	No Hit
GTGTGGGAGCAGGGAGCAAGCTTTGGCCAGAGCCAAGGGTGCA	2217	0.1219804743208268	No Hit
GTATGCAGCTGATCTCCGTAGATACATCAACATGCTGACCAGG	2144	0.11796397697061464	No Hit
GGGTACACTGGCTCCAGTGGGGCTCCCTGGGCACCCAGCAGTG	2016	0.11092135147983168	No Hit
AGTGTACCCAGGGGACAATGCCACACCAGAGCAGATGGCCCAG	1900	0.10453897212880962	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	5.5020511646741906E-5	0.0
2	0.0	0.0	0.0	7.152666514076447E-4	0.0
3	5.5020511646741906E-5	0.0	0.0	0.0013204922795218058	0.0
4	5.5020511646741906E-5	0.0	0.0	0.0024759230241033856	0.0
5	1.1004102329348381E-4	0.0	0.0	0.0025309435357501278	0.0
6	1.1004102329348381E-4	0.0	0.0	0.0025309435357501278	0.0
7	2.2008204658696763E-4	0.0	0.0	0.002861066605630579	0.0
8	2.2008204658696763E-4	0.0	0.0	0.0032462101871577726	0.0
9	2.2008204658696763E-4	0.0	0.0	0.005392010141380707	0.0
10	3.851435815271933E-4	0.0	0.0	0.007592830607250383	0.0
11	3.851435815271933E-4	0.0	0.0	0.008693240840185221	0.0
12	3.851435815271933E-4	0.0	0.0	0.010288835677940736	0.0
13	3.851435815271933E-4	0.0	0.0	0.01127920488758209	0.0
14	3.851435815271933E-4	0.0	0.0	0.012599697167103897	0.0
15	7.152666514076447E-4	0.0	0.0	0.014800517632973572	0.0
16	7.152666514076447E-4	0.0	0.0	0.018872035494832472	0.0
17	9.903692096413542E-4	0.0	0.0	0.02426404563621318	0.0
18	9.903692096413542E-4	0.0	0.0	0.026850009683610048	0.0
19	9.903692096413542E-4	0.0	0.0	0.03147173266193637	0.0
20	9.903692096413542E-4	0.0	0.0	0.034717942849094144	0.0
21	9.903692096413542E-4	0.0	0.0	0.04209069140975756	0.0
22	0.00115543074458158	0.0	0.0	0.054690388576861454	0.0
23	0.00115543074458158	0.0	0.0	0.07807410602672676	0.0
24	0.001210451256228322	0.0	0.0	0.11367237706216878	0.0
25	0.001210451256228322	0.0	0.0	0.120659982041305	0.0
26	0.0012654717678750639	0.0	0.0	0.1355155201859253	0.0
27	0.0012654717678750639	0.0	0.0	0.14938068912090427	0.0
28	0.0013755127911685477	0.0	0.0	0.1805222987129602	0.0
29	0.0013755127911685477	0.0	0.0	0.23433235910347378	5.5020511646741906E-5
30	0.0013755127911685477	0.0	0.0	0.30949037801292323	5.5020511646741906E-5
31	0.0014305333028152894	0.0	0.0	0.5949367924362202	5.5020511646741906E-5
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGCTAA	45	1.3236259E-4	24.666666	14
TCTATAC	175	0.0	19.028572	3
CGTTATA	50	0.0070364173	18.5	2
CTAGCGG	250	0.0	17.760002	29
TCTAGCG	255	0.0	17.411764	28
CGAATTG	100	5.881573E-6	16.650002	14
GCAGTCG	295	0.0	16.305084	9
CGAACGA	125	1.6591548E-7	16.28	16
CAGATCG	80	3.383918E-4	16.1875	9
CGCAATA	275	0.0	16.145454	36
TACCGTC	185	1.8189894E-11	16.0	7
AGTCGGT	305	0.0	15.770493	11
CTTATAC	1275	0.0	15.52549	37
CCGGTCC	325	0.0	15.369231	9
CGGTCCA	325	0.0	15.369231	10
GCTTTAT	735	0.0	15.353742	1
ATACCGT	195	4.1836756E-11	15.179486	6
CTTGCGC	345	0.0	15.014493	3
CGTCGTA	185	3.0559022E-10	15.0	10
GGTATCA	3655	0.0	14.83037	1
>>END_MODULE