FastQCFastQC Report
Fri 10 Feb 2017
ERR1630493.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630493.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1662511
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT104260.6271236701591748No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT101580.6110034760672259No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT74800.4499218351036474No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT58390.3512157212794382No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16830.10123241289832068No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACCG400.001931548123.1255
CGTTATA450.003826515520.5555572
ATACGAC500.007036169518.56
CGCAAGT500.007036169518.512
CGGTTTA852.7242999E-517.41176415
CGACCCG1757.2759576E-1216.9142865
TATCCGG700.00259314615.85714310
TACGTTA1302.5907866E-715.65384619
GGTATCA56700.015.5634921
TAAACCG750.00410543214.7999995
CGTAGTA750.00410543214.7999992
ACCGTGT1508.107236E-814.7999998
GCTTTAT5300.014.66037751
TATTAGA3050.014.5573772
ACGTTAA1405.9989725E-714.53571520
GTCGGTT2059.276846E-1114.43902412
AAGACGG2450.014.346945
TTAACGG1051.6563335E-414.09523835
TATACTG1854.9021764E-914.05
GTATAGA2252.7284841E-1113.97777751
Produced by FastQC (version 0.11.5)