##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1630493.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1662511
Sequences flagged as poor quality	0
Sequence length	43
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.09534553455586	33.0	31.0	34.0	30.0	34.0
2	32.2569402548314	33.0	31.0	34.0	30.0	34.0
3	32.3610321976817	34.0	31.0	34.0	30.0	34.0
4	35.933927053715735	37.0	35.0	37.0	35.0	37.0
5	35.895455127815694	37.0	35.0	37.0	35.0	37.0
6	35.978301496952504	37.0	35.0	37.0	35.0	37.0
7	35.926058233599655	37.0	35.0	37.0	35.0	37.0
8	35.93727199398982	37.0	35.0	37.0	35.0	37.0
9	37.640784933152325	39.0	37.0	39.0	35.0	39.0
10	37.562758983248834	39.0	37.0	39.0	35.0	39.0
11	37.66157577303248	39.0	37.0	39.0	35.0	39.0
12	37.61147565339417	39.0	37.0	39.0	35.0	39.0
13	37.65485882499424	39.0	37.0	39.0	35.0	39.0
14	38.946089980758025	40.0	38.0	41.0	36.0	41.0
15	38.95621562804697	40.0	38.0	41.0	36.0	41.0
16	38.91129201551148	40.0	38.0	41.0	35.0	41.0
17	38.91936654855216	40.0	38.0	41.0	36.0	41.0
18	38.920398120674086	40.0	38.0	41.0	35.0	41.0
19	38.98336311759742	40.0	38.0	41.0	35.0	41.0
20	38.95731998164223	40.0	38.0	41.0	35.0	41.0
21	38.91734550929287	40.0	38.0	41.0	35.0	41.0
22	38.84217728484202	40.0	38.0	41.0	35.0	41.0
23	38.786303970319594	40.0	38.0	41.0	35.0	41.0
24	38.739061576133935	40.0	38.0	41.0	35.0	41.0
25	38.684150059759006	40.0	38.0	41.0	35.0	41.0
26	38.54319520291896	40.0	38.0	41.0	34.0	41.0
27	38.40728692922934	40.0	38.0	41.0	34.0	41.0
28	38.30463377385172	40.0	38.0	41.0	34.0	41.0
29	38.22689293484374	40.0	38.0	41.0	34.0	41.0
30	38.130035229842086	40.0	37.0	41.0	33.0	41.0
31	38.06102215263538	40.0	37.0	41.0	33.0	41.0
32	37.948705903299285	40.0	37.0	41.0	33.0	41.0
33	37.8496521226025	40.0	37.0	41.0	33.0	41.0
34	37.770894147467295	40.0	37.0	41.0	33.0	41.0
35	37.69098610475359	40.0	37.0	41.0	33.0	41.0
36	37.62589661060889	40.0	37.0	41.0	33.0	41.0
37	37.47007989721572	40.0	36.0	41.0	33.0	41.0
38	37.30291468748177	40.0	36.0	41.0	32.0	41.0
39	37.21170085491163	40.0	36.0	41.0	32.0	41.0
40	37.003467646229105	40.0	36.0	41.0	31.0	41.0
41	36.91829708194412	40.0	35.0	41.0	31.0	41.0
42	36.75213818134136	40.0	35.0	41.0	31.0	41.0
43	35.98707617573658	39.0	35.0	40.0	29.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	4.0
10	14.0
11	5.0
12	5.0
13	2.0
14	2.0
15	5.0
16	11.0
17	14.0
18	34.0
19	76.0
20	184.0
21	404.0
22	726.0
23	1218.0
24	2205.0
25	3577.0
26	5527.0
27	8306.0
28	12301.0
29	17279.0
30	23246.0
31	30341.0
32	38969.0
33	50062.0
34	66556.0
35	88929.0
36	124949.0
37	200008.0
38	356654.0
39	630898.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.68920807140524	19.437525526146896	12.179949486048514	23.69331691639935
2	18.624478274128712	21.178326038143506	34.858656574302366	25.338539113425412
3	20.724494454472783	24.0882616716521	29.33382094915462	25.853422924720498
4	13.916058299764632	15.386003461029732	34.518087399120965	36.17985084008467
5	13.842615176681539	38.166003112159856	33.920978567961356	14.070403143197248
6	34.537515842000445	34.557184884791745	15.998630986501745	14.906668286706074
7	28.530818743454933	31.13362858952512	21.740006532287605	18.59554613473234
8	28.09052090482409	32.683272471580636	20.085160338788736	19.14104628480654
9	26.75134179563323	13.174048171711345	20.919500683003	39.15510934965242
10	17.1782322041779	27.42051030038298	32.93391742971926	22.467340065719867
11	34.459020120769125	22.17663522226319	21.720277339518354	21.644067317449327
12	21.665420559623367	26.732695302467175	29.22085929055507	22.381024847354393
13	29.99312485751974	20.202753545690825	26.11970687712743	23.684414719662005
14	22.555459783423988	21.683706152921694	26.757477093384647	29.00335697026967
15	25.7153787253137	28.02826567764063	23.64706158335193	22.609294013693745
16	24.205734578598275	26.667492726363918	25.749363462858295	23.377409232179517
17	22.607609814311004	27.15482784775559	26.783040834015537	23.454521503917867
18	22.36580690293177	25.284825183111572	28.83722273115787	23.51214518279879
19	22.9636976838048	25.954113987817223	28.869342819385857	22.21284550899212
20	23.89367649296757	25.043683921489844	28.534427742132234	22.528211843410357
21	24.302876792995658	25.474778813493565	27.930642263419607	22.29170213009117
22	24.040081539310115	25.476102113008576	27.967333749972177	22.51648259770913
23	23.267334772521806	25.614627512239018	27.942130909209023	23.175906806030156
24	22.84442027751997	26.359705289168012	28.016656731895306	22.779217701416712
25	23.13578677073415	26.11122573023577	27.81725955497437	22.93572794405571
26	22.950284238720826	26.54388452166632	27.929860313706197	22.575970925906656
27	23.105771931734587	25.95140723880925	27.84468794492187	23.098132884534298
28	22.48640760873161	26.196819148865778	28.46399211794689	22.852781124455717
29	22.368393351983837	26.694139166597996	28.62651735838139	22.31095012303678
30	22.40418258886708	26.94737057378869	28.161678328744895	22.48676850859934
31	22.428603479916827	27.14556475115052	27.83765039750113	22.588181371431524
32	21.78596111544525	26.73281560242308	28.07247591143758	23.408747370694087
33	21.6473154162589	26.72024425703048	28.577434976370082	23.055005350340537
34	22.89043501065557	26.034474358365145	28.210339660910517	22.86475097006877
35	22.33067931580603	26.20060859747695	28.447631323943117	23.021080762773902
36	21.74920947891473	26.88331084726657	28.17882107246208	23.188658601356625
37	22.33988226243315	25.7422657654596	28.641434552914234	23.276417419193017
38	21.97633579567293	25.739679316407532	28.808471041695366	23.475513846224175
39	21.945659306915864	25.67459704026018	29.110905130853272	23.26883852197068
40	21.352339924367417	25.584372073327632	29.674510424291928	23.388777578013016
41	20.77706553520548	25.830986982943273	29.89598264312236	23.495964838728884
42	21.0156804977531	25.80638564195966	30.022778796651572	23.155155063635668
43	20.80455407513093	25.376433599537084	29.784945783817367	24.034066541514612
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	524.0
1	489.5
2	455.0
3	1247.5
4	2040.0
5	2040.0
6	2801.0
7	3562.0
8	3530.0
9	3498.0
10	5052.0
11	6606.0
12	6606.0
13	11114.5
14	15623.0
15	20664.0
16	25705.0
17	24062.5
18	22420.0
19	22420.0
20	25649.5
21	28879.0
22	26179.5
23	23480.0
24	26070.0
25	28660.0
26	28660.0
27	32293.5
28	35927.0
29	40114.0
30	44301.0
31	49303.0
32	54305.0
33	54305.0
34	59775.0
35	65245.0
36	70689.0
37	76133.0
38	81860.5
39	87588.0
40	87588.0
41	90895.5
42	94203.0
43	95497.0
44	96791.0
45	99392.0
46	101993.0
47	101993.0
48	105035.5
49	108078.0
50	107052.0
51	106026.0
52	104600.5
53	103175.0
54	103175.0
55	101208.5
56	99242.0
57	95280.0
58	91318.0
59	88751.5
60	86185.0
61	86185.0
62	79008.0
63	71831.0
64	64687.0
65	57543.0
66	49891.5
67	42240.0
68	42240.0
69	36648.0
70	31056.0
71	26241.5
72	21427.0
73	16568.0
74	11709.0
75	11709.0
76	8687.0
77	5665.0
78	4562.5
79	3460.0
80	2857.0
81	2254.0
82	2254.0
83	1781.0
84	1308.0
85	1120.5
86	933.0
87	814.0
88	695.0
89	695.0
90	535.5
91	376.0
92	205.0
93	34.0
94	21.5
95	9.0
96	9.0
97	8.0
98	7.0
99	4.5
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1662511.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	62.0914720565623
#Duplication Level	Percentage of deduplicated	Percentage of total
1	87.73178580994818	54.47395727090707
2	7.813618143726314	9.703181052636612
3	1.7943500353678272	3.34241505242199
4	0.7213038303119772	1.7914726649642998
5	0.39108323962746194	1.2141467022559205
6	0.2503752202775213	0.9327699596110408
7	0.17694720593043206	0.7690838744761339
8	0.13778456481538678	0.6844197164848149
9	0.1083905046219505	0.6057113390037475
>10	0.6982926349913706	8.668035080345101
>50	0.0910586388274722	3.968092518057589
>100	0.0793753988278246	9.416257881936598
>500	0.004371806425564222	1.7308376147890083
>1k	8.743612851128443E-4	0.6545006838065291
>5k	1.9430250780285427E-4	0.8034372910248077
>10k+	1.9430250780285427E-4	1.2416812972786724
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	10426	0.6271236701591748	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	10158	0.6110034760672259	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	7480	0.4499218351036474	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5839	0.3512157212794382	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1683	0.10123241289832068	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	6.014997795503308E-5	0.0
2	0.0	0.0	0.0	4.2104984568523153E-4	0.0
3	0.0	0.0	0.0	6.014997795503308E-4	0.0
4	0.0	0.0	0.0	0.0010826996031905955	0.0
5	0.0	0.0	0.0	0.0012029995591006616	0.0
6	0.0	0.0	0.0	0.0012631495370556947	0.0
7	0.0	0.0	0.0	0.0013834494929657608	0.0
8	0.0	0.0	0.0	0.001443599470920794	0.0
9	0.0	0.0	0.0	0.0026465990300214553	0.0
10	0.0	0.0	0.0	0.0034285487434368854	0.0
11	0.0	0.0	0.0	0.00469169828049258	0.0
12	0.0	0.0	0.0	0.006075147773458341	0.0
13	0.0	0.0	0.0	0.007157847376648936	0.0
14	0.0	0.0	0.0	0.00878189678143483	0.0
15	0.0	0.0	0.0	0.010706696075995888	0.0
16	0.0	0.0	0.0	0.016300644025813965	0.0
17	0.0	0.0	0.0	0.025563740630889058	0.0
18	0.0	0.0	0.0	0.028811839440460844	0.0
19	0.0	0.0	0.0	0.03458623732414402	0.0
20	0.0	0.0	0.0	0.03843583591326614	0.0
21	0.0	0.0	0.0	0.047398182628566064	0.0
22	0.0	0.0	0.0	0.06778902515532229	0.0
23	0.0	0.0	0.0	0.1002098632730851	0.0
24	0.0	0.0	0.0	0.1519989942923686	0.0
25	0.0	0.0	0.0	0.16324704016995978	0.0
26	0.0	0.0	0.0	0.18387848260853612	0.0
27	0.0	0.0	0.0	0.2049309748927977	0.0
28	0.0	0.0	0.0	0.2505848081606678	0.0
29	0.0	0.0	0.0	0.3251707808249088	0.0
30	0.0	0.0	0.0	0.43723018975513545	0.0
31	0.0	0.0	0.0	0.7961451082128178	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TATACCG	40	0.0019315481	23.125	5
CGTTATA	45	0.0038265155	20.555557	2
ATACGAC	50	0.0070361695	18.5	6
CGCAAGT	50	0.0070361695	18.5	12
CGGTTTA	85	2.7242999E-5	17.411764	15
CGACCCG	175	7.2759576E-12	16.914286	5
TATCCGG	70	0.002593146	15.857143	10
TACGTTA	130	2.5907866E-7	15.653846	19
GGTATCA	5670	0.0	15.563492	1
TAAACCG	75	0.004105432	14.799999	5
CGTAGTA	75	0.004105432	14.799999	2
ACCGTGT	150	8.107236E-8	14.799999	8
GCTTTAT	530	0.0	14.6603775	1
TATTAGA	305	0.0	14.557377	2
ACGTTAA	140	5.9989725E-7	14.535715	20
GTCGGTT	205	9.276846E-11	14.439024	12
AAGACGG	245	0.0	14.34694	5
TTAACGG	105	1.6563335E-4	14.095238	35
TATACTG	185	4.9021764E-9	14.0	5
GTATAGA	225	2.7284841E-11	13.9777775	1
>>END_MODULE