FastQCFastQC Report
Fri 10 Feb 2017
ERR1630492.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630492.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1281625
Sequences flagged as poor quality0
Sequence length43
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT48900.38154686433239055No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT44080.3439383595045353No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT32680.25498878377060374No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22260.17368575051204524No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGCTTA500.007035313618.49999828
CTAGATA1351.1514203E-917.8148143
GTATAGA1502.5102054E-1017.2666661
CGAACGA1107.8093217E-716.81818216
AGTCGGT1801.0913936E-1116.44444511
GTAAGAC803.3829446E-416.18753
TCGCACG1059.344694E-615.85714222
GTGCTAC1059.344694E-615.8571421
CAATACG957.060685E-515.5789484
GGTATCA24400.015.5430331
ATAACGA1201.935825E-615.41666712
CGCTTAT855.3647504E-415.23529326
ATACACC1353.972873E-715.0740734
GCAGTCG2109.094947E-1214.976199
TCTTATA13600.014.82720537
CTGTCGC750.00410474914.89
CCGCTTA750.00410474914.825
CGTTAAC750.00410474914.82
AAACGTG750.00410474914.85
TAATACT2006.184564E-1114.7999994
Produced by FastQC (version 0.11.5)