FastQCFastQC Report
Fri 10 Feb 2017
ERR1630491.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630491.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences588752
Sequences flagged as poor quality0
Sequence length43
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT56830.965262113759274No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT50330.8548590917737859No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT41380.7028426230399217No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33860.5751148191428649No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8990.15269587194608256No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8880.150827513112482No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT8050.13672989645895045No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCTAAG405.935282E-527.7500021
GCCGTCT1000.027.75000236
GGTCTAC358.8642363E-426.428571
ACCGTGT400.00193007423.1250028
TTGCCGA502.699933E-422.210
CCGTCTT1301.8189894E-1221.34615337
AGCCGTT450.003823625220.55555514
TTAGACA450.003823625220.5555554
GTTGCGG450.003823625220.5555559
GCCGAGT555.139E-420.18181812
GGTATCA28250.019.7115041
CTAAGCA951.6726517E-719.4736844
CGAGTTC609.2295214E-418.514
CCGAGTT609.2295214E-418.513
GGAACGC500.00703090518.58
TTGCGGT500.00703090518.510
TTTGCCG609.2295214E-418.59
GTCTTAC701.21791585E-418.51
GCTAAGC701.21791585E-418.53
CCGTTAA609.2295214E-418.516
Produced by FastQC (version 0.11.5)