FastQCFastQC Report
Fri 10 Feb 2017
ERR1630490.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630490.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences785116
Sequences flagged as poor quality0
Sequence length43
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT120331.5326397627866455No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT118621.5108595417747186No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT105361.3419673016471452No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT68770.8759215198773176No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA28850.3674616235053164No Hit
CTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCGAGATTGAGC25130.3200800900758614No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC22620.28811029198233123No Hit
TCTTAGAGGGACAAGTGGCGTTCAGCCACCCGAGATTGAGCAA19770.2518099236291198No Hit
TGCCTACCCTACGCCGGCAGGCGCGGGTAACCCGTTGAACCCC19710.2510457053479995No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT19380.2468425048018382No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATG19080.24302141339623698No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18450.23499712144447443No Hit
GGGTTCAACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGGCAC16540.21066950616214675No Hit
GCGCTACACTGACTGGCTCAGCGTGTGCCTACCCTACGCCGGC15490.1972956862425425No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15360.19563987996678195No Hit
AGTCAGTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATC14120.17984603549029698No Hit
CCTTAGATGTCCGGGGCTGCACGCGCGCTACACTGACTGGCTC13960.17780812007397634No Hit
GGTCTGTGATGCCCTTAGATGTCCGGGGCTGCACGCGCGCTAC13880.176789162365816No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGAT13460.1714396343979743No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13000.16558062757605246No Hit
GTACATGGGCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCAC12990.1654532578625324No Hit
CATCTAAGGGCATCACAGACCTGTTATTGCTCAATCTCGGGTG12630.16086794817581096No Hit
CTACCATGGTGACCACGGGTGACGGGGAATCAGGGTTCGATTC12300.15666474762964963No Hit
ACCCGAGATTGAGCAATAACAGGTCTGTGATGCCCTTAGATGT12000.1528436562240484No Hit
CCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAATC11830.15067837109420773No Hit
ACACTGACTGGCTCAGCGTGTGCCTACCCTACGCCGGCAGGCG11700.14902256481844722No Hit
ACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATC11440.14571095226692615No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTT11390.14507410369932597No Hit
GCCCCGGACATCTAAGGGCATCACAGACCTGTTATTGCTCAAT11310.14405514599116562No Hit
GGATTACTCCGGTCTGAACTCAGATCACGTAGGACTTTAATCG10540.13424767805012253No Hit
TCACAGACCTGTTATTGCTCAATCTCGGGTGGCTGAACGCCAC10330.1315729140662017No Hit
GTCCGGGGCTGCACGCGCGCTACACTGACTGGCTCAGCGTGTG10260.1306813260715614No Hit
CTCCGGAATCGAACCCTGATTCCCCGTCACCCGTGGTCACCAT10190.12978973807692112No Hit
CCATGGTAGGCACGGCGACTACCATCGAAAGTTGATAGGGCAG9890.1259686466713199No Hit
GGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGG9790.1246949495361195No Hit
ATTCCGGAGAGGGAGCCTGAGAAACGGCTACCACATCCAAGGA9390.11960016099531788No Hit
CCCCGATCCCCATCACGAATGGGGTTCAACGGGTTACCCGCGC9390.11960016099531788No Hit
ATTCGAACGTCTGCCCTATCAACTTTCGATGGTAGTCGCCGTG9300.11845383357363753No Hit
TAACAGGTCTGTGATGCCCTTAGATGTCCGGGGCTGCACGCGC9190.11705276672491709No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9180.11692539701139704No Hit
ACGCAGAGTACATGGGCAACTTCTTAGAGGGACAAGTGGCGTT9030.11501485130859643No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG9020.1148874815950764No Hit
GACAAGTGGCGTTCAGCCACCCGAGATTGAGCAATAACAGGTC8730.11119375990299522No Hit
AGATTGAGCAATAACAGGTCTGTGATGCCCTTAGATGTCCGGG8570.10915584448667458No Hit
GTTCAACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGGCACAC8550.1089011050596345No Hit
TCTAAGGGCATCACAGACCTGTTATTGCTCAATCTCGGGTGGC8420.10724529878387398No Hit
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA8380.10673581992979382No Hit
GAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGACATCTAAGG8320.10597160164867356No Hit
CGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGACATCT8140.10367894680531284No Hit
GTTATTGCTCAATCTCGGGTGGCTGAACGCCACTTGTCCCTCT8050.10253261938363248No Hit
TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8050.10253261938363248No Hit
CTCTAATACTGGTGATGCTAGAGGTGATGTTTTTGGTAAACAG7890.10049470396731183No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATATAGC352.3873512E-531.7142856
GAATAAC250.005495296329.5999981
ATCGTTT250.005495296329.59999829
AATAACG250.005495296329.5999982
TGTTTAC1250.026.6414
TAAAGAT358.866882E-426.4285724
CTAGAAC451.3226904E-424.66666624
TAACGCC451.3226904E-424.6666664
TTACCAA1400.023.78571317
TAGAACC551.9016637E-523.54545425
GTCCTGG400.001930644623.1250021
TCGTGTT400.001930644623.12500225
GTGCTTT400.001930644623.12500217
CTTTACG400.001930644623.12500235
GGCTCGA400.001930644623.12500231
ATCTTAC1450.022.9655171
CTTACCC1550.022.6774183
GTTTACC1500.022.215
ACATGGA753.7376412E-722.23
TACATAC502.701046E-422.1999993
Produced by FastQC (version 0.11.5)