FastQCFastQC Report
Fri 10 Feb 2017
ERR1630489.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630489.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1440065
Sequences flagged as poor quality0
Sequence length43
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT67100.46595118970324256No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT65240.45303510605424063No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT50210.3486648172131119No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT35700.24790547648890848No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTATAC11000.018.537
AGTCGGT1800.018.511
TAACGGC852.7238893E-517.41176436
CGAATTA1107.810977E-716.81818215
TAGTACT1107.810977E-716.8181824
AAGACGG2550.016.6862745
CGCAAGA2550.016.6862742
TTAACGG904.4472115E-516.44444535
CGGTGAT2300.016.08695614
CTTAACG700.002592920615.85714330
CGCTAAG700.002592920615.85714335
TACGTTA700.002592920615.85714319
GCAGTCG2100.015.8571429
GACGGAC2450.015.8571427
GGTATCA33950.015.3667161
CAGTCGG2300.015.28260910
TCTATGG4500.015.2111112
GCGTTAT1954.1836756E-1115.1794871
ACGCTAA750.004105075714.834
TACTCCG1508.104507E-814.85
Produced by FastQC (version 0.11.5)