FastQCFastQC Report
Fri 10 Feb 2017
ERR1630488.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630488.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1544434
Sequences flagged as poor quality0
Sequence length43
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT35440.2294691777052305No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT34290.22202308418488587No Hit
ACCTAGGCCTGGTCAGCATGTTGATGTATCTACGGAGATCAGC31600.20460570021121005No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT28460.18427462746870374No Hit
GTGTGGGAGCAGGGAGCAAGCTTTGGCCAGAGCCAAGGGTGCA24030.15559098025554993No Hit
GGCCTAGGTATGGGAAAAGACACAAAGAGGACACGCTGGCCTT23870.15455500202663242No Hit
GCTTTATTGAGCCTGTGTGGGAGCAGGGAGCAAGCTTTGGCCA22990.14885712176758606No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21410.13862683675702556No Hit
GTATGCAGCTGATCTCCGTAGATACATCAACATGCTGACCAGG19230.12451163338802435No Hit
GGGTACACTGGCTCCAGTGGGGCTCCCTGGGCACCCAGCAGTG17800.11525257796707403No Hit
AGTGTACCCAGGGGACAATGCCACACCAGAGCAGATGGCCCAG17210.11143240824794066No Hit
GCTGCATACTGGGCCATCTGCTCTGGTGTGGCATTGTCCCCTG15700.10165536371253157No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGGTGA2250.020.55555513
AGTCGGT2250.018.91111211
CGCCTTA1653.6379788E-1217.93939425
GCAGTCG2450.017.3673469
TATACTG2450.017.3673465
TTAGGAC2150.017.2093033
TAATACT2500.017.024
CGGTGAT2500.017.0214
GGTATCA19000.016.7473681
CTGTCGC1554.0199666E-1016.7096799
TTGCGCG1005.88042E-616.65000218
TGCGCTA904.44755E-516.4444455
CCTATAC1352.2208042E-816.4444453
GTCGGTG2700.016.44444512
CAGTCGG2600.016.36538510
TCCATAC1251.6586819E-716.283
GTCCTAT2750.016.1454541
TCTATAC1751.3096724E-1015.8571433
GCTTTAT8200.015.5670731
TCTAGCG1701.4861143E-915.23529428
Produced by FastQC (version 0.11.5)