##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1630488.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1544434
Sequences flagged as poor quality	0
Sequence length	43
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.013602394145686	33.0	31.0	34.0	30.0	34.0
2	32.1875282465939	33.0	31.0	34.0	30.0	34.0
3	32.300530161858646	34.0	31.0	34.0	30.0	34.0
4	35.888016580831554	37.0	35.0	37.0	35.0	37.0
5	35.83505866874208	37.0	35.0	37.0	35.0	37.0
6	35.905577059298096	37.0	35.0	37.0	35.0	37.0
7	35.85475779476494	37.0	35.0	37.0	35.0	37.0
8	35.85297526472481	37.0	35.0	37.0	35.0	37.0
9	37.56091746231953	39.0	37.0	39.0	35.0	39.0
10	37.45747892108047	39.0	37.0	39.0	35.0	39.0
11	37.5497496170118	39.0	37.0	39.0	35.0	39.0
12	37.48900891847758	39.0	37.0	39.0	35.0	39.0
13	37.532801013186706	39.0	37.0	39.0	35.0	39.0
14	38.82475586525549	40.0	38.0	41.0	35.0	41.0
15	38.84121237942185	40.0	38.0	41.0	35.0	41.0
16	38.810140154904644	40.0	38.0	41.0	35.0	41.0
17	38.79008814879755	40.0	38.0	41.0	35.0	41.0
18	38.769598441888746	40.0	38.0	41.0	35.0	41.0
19	38.8156127098989	40.0	38.0	41.0	35.0	41.0
20	38.788189071206666	40.0	38.0	41.0	35.0	41.0
21	38.73843427430373	40.0	38.0	41.0	35.0	41.0
22	38.672886636787325	40.0	38.0	41.0	34.0	41.0
23	38.63313356219819	40.0	38.0	41.0	34.0	41.0
24	38.577854411389545	40.0	38.0	41.0	34.0	41.0
25	38.53429670675471	40.0	38.0	41.0	34.0	41.0
26	38.40600763774949	40.0	38.0	41.0	34.0	41.0
27	38.28410731698473	40.0	38.0	41.0	34.0	41.0
28	38.192672525986865	40.0	38.0	41.0	34.0	41.0
29	38.13046915569069	40.0	38.0	41.0	33.0	41.0
30	38.0528251773789	40.0	38.0	41.0	33.0	41.0
31	38.02525326430265	40.0	37.0	41.0	33.0	41.0
32	37.95189370345382	40.0	37.0	41.0	33.0	41.0
33	37.87588592325732	40.0	37.0	41.0	33.0	41.0
34	37.828827907181534	40.0	37.0	41.0	33.0	41.0
35	37.77109284048396	40.0	37.0	41.0	33.0	41.0
36	37.74760591906161	40.0	37.0	41.0	33.0	41.0
37	37.628851734680794	40.0	37.0	41.0	33.0	41.0
38	37.4971957364316	40.0	37.0	41.0	32.0	41.0
39	37.450360455675025	40.0	37.0	41.0	32.0	41.0
40	37.27839519202504	40.0	36.0	41.0	31.0	41.0
41	37.24248753912437	40.0	36.0	41.0	31.0	41.0
42	37.117797199491854	39.0	36.0	41.0	31.0	41.0
43	36.3137809708929	38.0	35.0	40.0	30.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	1.0
9	2.0
10	5.0
11	2.0
12	2.0
13	1.0
14	2.0
15	2.0
16	7.0
17	22.0
18	28.0
19	68.0
20	173.0
21	373.0
22	630.0
23	1170.0
24	1968.0
25	3254.0
26	4900.0
27	7647.0
28	11114.0
29	15356.0
30	21032.0
31	28044.0
32	35890.0
33	46931.0
34	62393.0
35	84431.0
36	119536.0
37	187839.0
38	368433.0
39	543177.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.78197190686038	18.669687406519152	11.527459250443851	25.020881436176616
2	19.247115771862056	20.228899389679324	34.137036610175635	26.386948228282982
3	20.989372158344093	23.02163770028373	28.341256408496573	27.6477337328756
4	14.430917734263815	14.37834183914625	34.50921178891426	36.68152863767568
5	14.559573280567509	36.90406971097502	34.46887338662578	14.067483621831686
6	36.54898817301355	34.28162032174894	14.908827440991326	14.26056406424619
7	30.071728542624676	29.49073900211987	21.26015096792741	19.177381487328045
8	28.468487484735512	32.276678705597	19.550463147016966	19.704370662650526
9	27.030161211162145	13.247895345479316	19.31302988667693	40.40891355668161
10	17.074021939428942	26.53230892352797	32.82166800264692	23.57200113439616
11	36.63128369357318	21.308129709654153	21.364072534015698	20.69651406275697
12	23.386366785502002	24.771728672121956	28.408077004261756	23.433827538114286
13	31.054548138670867	19.411771561620633	24.724526914066903	24.8091533856416
14	23.130221168402144	20.284583219483643	25.164364420881697	31.42083119123252
15	26.07719073783664	27.900836163928016	21.92660871231791	24.09536438591743
16	25.738296359702	26.073564814035432	23.53082099979669	24.657317826465878
17	24.512604617613963	25.837750269678082	24.822491605338914	24.827153507369044
18	24.048421622419607	24.69144035938085	26.688612138815905	24.571525879383646
19	25.307653159668845	24.708275005600758	26.37963163204125	23.60444020268914
20	25.920822773909407	24.237940889672206	25.391696893489783	24.449539442928607
21	25.65736056056782	24.486122424137257	25.591511194392254	24.265005820902672
22	25.5478706114991	24.619051380635234	25.958506481986284	23.874571525879386
23	25.012982102181127	24.529504012473176	25.462531904892018	24.99498198045368
24	24.570036660679577	25.032018202137486	25.969125258832687	24.428819878350254
25	25.00255757125264	25.059730619761027	25.29263147534955	24.645080333636788
26	24.87208906304834	25.361524027572564	25.511417127569064	24.254969781810036
27	25.026644065074972	24.29071103070769	25.762382853524336	24.920262050693005
28	24.509302437009286	24.984168957689352	25.682418283979764	24.824110321321598
29	24.54692139644685	25.379394652021386	26.176191407337573	23.897492544194186
30	24.723490935837983	25.23099077072895	25.329149707918887	24.716368585514175
31	24.118350152871535	25.90249890898543	25.797347118750295	24.181803819392737
32	23.955507325013564	25.094371141790457	25.588468008344805	25.361653524851175
33	23.526029600487945	25.59228817806394	25.992305271704712	24.8893769497434
34	25.350063518415162	24.28410666949834	25.731627249853346	24.634202562233153
35	24.424611216795277	24.555921457310575	26.05433446816115	24.965132857732993
36	23.703117128993533	25.63916619292246	26.116363664617587	24.54135301346642
37	24.58913750927524	24.312466573514957	26.553675974499395	24.544719942710405
38	24.486381418694485	24.699015950179806	26.118370872436113	24.696231758689592
39	24.18840818060208	24.461517941200466	26.485689903226685	24.864383974970767
40	23.466137109128653	24.176753425526762	27.062082290340665	25.295027175003916
41	22.57415985403067	24.580784934804594	27.668582794732572	25.176472416432166
42	23.409676295652645	24.367632414204817	27.838224229717813	24.384467060424726
43	23.593368185367584	23.913550206742407	27.518948689293293	24.974132918596716
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	135.0
1	156.0
2	177.0
3	529.0
4	881.0
5	881.0
6	1180.0
7	1479.0
8	1359.5
9	1240.0
10	1769.0
11	2298.0
12	2298.0
13	3814.0
14	5330.0
15	7208.5
16	9087.0
17	9148.5
18	9210.0
19	9210.0
20	11342.0
21	13474.0
22	14017.0
23	14560.0
24	17341.0
25	20122.0
26	20122.0
27	23353.5
28	26585.0
29	30729.0
30	34873.0
31	39382.0
32	43891.0
33	43891.0
34	49258.5
35	54626.0
36	60123.0
37	65620.0
38	70692.5
39	75765.0
40	75765.0
41	79146.0
42	82527.0
43	83987.0
44	85447.0
45	88271.5
46	91096.0
47	91096.0
48	96131.0
49	101166.0
50	101955.5
51	102745.0
52	103621.0
53	104497.0
54	104497.0
55	101991.0
56	99485.0
57	98111.0
58	96737.0
59	98214.5
60	99692.0
61	99692.0
62	91908.5
63	84125.0
64	77555.5
65	70986.0
66	61751.5
67	52517.0
68	52517.0
69	46066.5
70	39616.0
71	33298.0
72	26980.0
73	19878.5
74	12777.0
75	12777.0
76	9455.0
77	6133.0
78	4851.0
79	3569.0
80	2849.5
81	2130.0
82	2130.0
83	1645.0
84	1160.0
85	977.0
86	794.0
87	687.5
88	581.0
89	581.0
90	426.5
91	272.0
92	151.5
93	31.0
94	23.0
95	15.0
96	15.0
97	9.0
98	3.0
99	1.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1544434.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	62.01574443894968
#Duplication Level	Percentage of deduplicated	Percentage of total
1	88.96600710976081	55.17293160672707
2	6.9455305464746475	8.614644947261805
3	1.6553694311922298	3.0797690279060044
4	0.7040595549898903	1.7465110972821472
5	0.3615257541191238	1.1210144387775072
6	0.23456209565333577	0.8727925787461044
7	0.15571458319821563	0.6759729057926678
8	0.11757096280095962	0.5833000626004458
9	0.08911320832884781	0.4973779758471038
>10	0.6030322907664315	7.4291355165857516
>50	0.07366183493496163	3.211274649791739
>100	0.08285439651048168	10.51209923170853
>500	0.008379604777214944	3.548484076149319
>1k	0.00261862649287967	2.934691884823877
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	3544	0.2294691777052305	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	3429	0.22202308418488587	No Hit
ACCTAGGCCTGGTCAGCATGTTGATGTATCTACGGAGATCAGC	3160	0.20460570021121005	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	2846	0.18427462746870374	No Hit
GTGTGGGAGCAGGGAGCAAGCTTTGGCCAGAGCCAAGGGTGCA	2403	0.15559098025554993	No Hit
GGCCTAGGTATGGGAAAAGACACAAAGAGGACACGCTGGCCTT	2387	0.15455500202663242	No Hit
GCTTTATTGAGCCTGTGTGGGAGCAGGGAGCAAGCTTTGGCCA	2299	0.14885712176758606	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2141	0.13862683675702556	No Hit
GTATGCAGCTGATCTCCGTAGATACATCAACATGCTGACCAGG	1923	0.12451163338802435	No Hit
GGGTACACTGGCTCCAGTGGGGCTCCCTGGGCACCCAGCAGTG	1780	0.11525257796707403	No Hit
AGTGTACCCAGGGGACAATGCCACACCAGAGCAGATGGCCCAG	1721	0.11143240824794066	No Hit
GCTGCATACTGGGCCATCTGCTCTGGTGTGGCATTGTCCCCTG	1570	0.10165536371253157	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	7.122350323807945E-4	0.0
3	0.0	0.0	0.0	0.0011654755075322092	0.0
4	0.0	0.0	0.0	0.0022014537364497284	0.0
5	0.0	0.0	0.0	0.0027841914902158333	0.0
6	0.0	0.0	0.0	0.0027841914902158333	0.0
7	0.0	0.0	0.0	0.003366929243981938	0.0
8	0.0	0.0	0.0	0.0034316778832892826	0.0
9	0.0	0.0	0.0	0.005762628898353701	0.0
10	0.0	0.0	0.0	0.008546820388569534	0.0
11	0.0	0.0	0.0	0.009323804060257674	0.0
12	0.0	0.0	0.0	0.010230285010560503	0.0
13	0.0	0.0	0.0	0.010554028207097228	0.0
14	0.0	0.0	0.0	0.010942520042941297	0.0
15	0.0	0.0	0.0	0.012237492829088197	0.0
16	0.0	0.0	0.0	0.014309449286923235	6.474863930734496E-5
17	0.0	0.0	0.0	0.017223138055753758	6.474863930734496E-5
18	0.0	0.0	0.0	0.018971351317052073	6.474863930734496E-5
19	0.0	0.0	0.0	0.021237553692809146	6.474863930734496E-5
20	0.0	0.0	0.0	0.023309510150644183	6.474863930734496E-5
21	0.0	0.0	0.0	0.026676439394626123	1.2949727861468992E-4
22	0.0	0.0	0.0	0.03386353835774141	1.2949727861468992E-4
23	0.0	0.0	0.0	0.0436405828931505	1.2949727861468992E-4
24	0.0	0.0	0.0	0.055489583886394626	1.2949727861468992E-4
25	0.0	0.0	0.0	0.05969824544137205	1.2949727861468992E-4
26	0.0	0.0	0.0	0.06604361209349185	1.2949727861468992E-4
27	0.0	0.0	0.0	0.07549691343236421	1.2949727861468992E-4
28	0.0	0.0	0.0	0.09848268038647168	1.2949727861468992E-4
29	0.0	0.0	0.0	0.13402968336620405	1.2949727861468992E-4
30	0.0	0.0	0.0	0.18343289515770825	1.2949727861468992E-4
31	0.0	0.0	0.0	0.3880385953689183	1.2949727861468992E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGGTGA	225	0.0	20.555555	13
AGTCGGT	225	0.0	18.911112	11
CGCCTTA	165	3.6379788E-12	17.939394	25
GCAGTCG	245	0.0	17.367346	9
TATACTG	245	0.0	17.367346	5
TTAGGAC	215	0.0	17.209303	3
TAATACT	250	0.0	17.02	4
CGGTGAT	250	0.0	17.02	14
GGTATCA	1900	0.0	16.747368	1
CTGTCGC	155	4.0199666E-10	16.709679	9
TTGCGCG	100	5.88042E-6	16.650002	18
TGCGCTA	90	4.44755E-5	16.444445	5
CCTATAC	135	2.2208042E-8	16.444445	3
GTCGGTG	270	0.0	16.444445	12
CAGTCGG	260	0.0	16.365385	10
TCCATAC	125	1.6586819E-7	16.28	3
GTCCTAT	275	0.0	16.145454	1
TCTATAC	175	1.3096724E-10	15.857143	3
GCTTTAT	820	0.0	15.567073	1
TCTAGCG	170	1.4861143E-9	15.235294	28
>>END_MODULE