FastQCFastQC Report
Fri 10 Feb 2017
ERR1630487.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630487.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1399464
Sequences flagged as poor quality0
Sequence length43
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT67070.47925491473878573No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT65870.4706802032778264No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT56750.40551239617453544No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT37780.2699604991625365No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTATA1254.129106E-1019.241
CCGGACT650.001580166317.0769234
CGCCTTA2100.016.73809425
CGAACGA2000.016.6516
CTAGTAC700.00259287215.8571423
ACACCGT957.061431E-515.5789486
TTGCACA1902.7284841E-1115.5789484
CTAATAC2500.015.543
CTTATAC8550.015.36257337
TTACACT1701.4861143E-915.2352944
TCTTATA14250.015.18947437
GGTATCA33800.015.1065091
TACCGTC1508.1039616E-814.87
ACGAACG2251.8189894E-1214.815
GCAGTCG2550.014.5098049
ATTGCCG2101.364242E-1014.09523811
TCGTAGA800.006299523613.8750012
GGCCGCA3750.013.81333333
TCCGAAA2151.9645086E-1013.76744221
TCTATGG4300.013.7674422
Produced by FastQC (version 0.11.5)