FastQCFastQC Report
Fri 10 Feb 2017
ERR1630486.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630486.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences810643
Sequences flagged as poor quality0
Sequence length43
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT26290.3243104547871258No Hit
ACCTAGGCCTGGTCAGCATGTTGATGTATCTACGGAGATCAGC21540.2657149941466219No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT20840.2570798736311792No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT18070.22290946816292742No Hit
GGCCTAGGTATGGGAAAAGACACAAAGAGGACACGCTGGCCTT17400.21464442424100372No Hit
GTGTGGGAGCAGGGAGCAAGCTTTGGCCAGAGCCAAGGGTGCA15840.1954004413780172No Hit
GCTTTATTGAGCCTGTGTGGGAGCAGGGAGCAAGCTTTGGCCA15380.1897259336107263No Hit
GTATGCAGCTGATCTCCGTAGATACATCAACATGCTGACCAGG13350.16468408411594251No Hit
GGGTACACTGGCTCCAGTGGGGCTCCCTGGGCACCCAGCAGTG12810.15802270543260102No Hit
AGTGTACCCAGGGGACAATGCCACACCAGAGCAGATGGCCCAG11800.1455634601174623No Hit
CCTCTGCCTCTCCCTGCTGCTCCTGTCCACCTGCGTGGCTCTG11470.14149261758875362No Hit
GCTGCATACTGGGCCATCTGCTCTGGTGTGGCATTGTCCCCTG11180.13791521051807024No Hit
CTGTTACTACAGCCACTGCTGGGTGCCCAGGGAGCCCCACTGG9930.12249535245477973No Hit
GCTCATGGAGTCGTAGGAGACAGAAGGTGGCATTATAAGTCCA9900.12212527586126076No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9640.11891794538409634No Hit
GTCTAGTGCCCATTTACTCTGGACTCCGGATGGCTGCCGCACG9360.11546389717791926No Hit
AGGCAGAGGCGTGCGGCAGCCATCCGGAGTCCAGAGTAAATGG8770.10818572417204614No Hit
GTGCAGAGGGGAGAGCTGGGCTGGCGCTGCTCATGGAGTCGTA8690.10719885325599554No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGACGG1151.3460522E-1020.9130445
CGAACGT806.954906E-720.81254
GCAGTCG1450.020.4137949
TTAACGG555.1412976E-420.18181835
CGGAAAC656.898924E-519.92307729
GTTTAGA656.898924E-519.9230771
GTATTAG1950.018.974361
TCGGAAA701.218635E-418.528
CGGTGAT1501.2732926E-1118.514
CGCAAGA1306.9485395E-1018.52
CATTAGC500.00703313818.4999982
CAGTCGG1653.6379788E-1217.93939410
TCGGTGA1653.6379788E-1217.93939413
GACGGAC1451.546141E-1017.8620687
ACGGACC1351.1496013E-917.8148148
TTTCGGA1156.3979314E-817.69565230
GCTTTAT5800.017.5431041
AGTCGGT1705.456968E-1217.41176411
GTACTAT752.0663197E-417.2666666
TGTAGGG752.0663197E-417.2666662
Produced by FastQC (version 0.11.5)