FastQCFastQC Report
Fri 10 Feb 2017
ERR1630485.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630485.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences311413
Sequences flagged as poor quality0
Sequence length43
%GC30

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT170895.48756795637947No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT144034.625047766149775No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT118803.8148696425646973No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT80802.594625144101242No Hit
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA23520.7552671211542229No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21880.7026039375363263No Hit
GTACATGGGAATGGTATCAACGCAAAAAAAAAAAAAAAAAAAA19270.6187924075102837No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT19150.6149390038309255No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16740.53754981327048No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15850.5089704026485729No Hit
GAATGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA13240.42515887262253016No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG11880.3814869642564697No Hit
GTACATGGGAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAA11650.374101273871033No Hit
CTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGT11640.3737801568977532No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10000.32111697327985667No Hit
GTACATGGGAAGCAGAGGTATCAACGCAAAAAAAAAAAAAAAA9180.2947853814709084No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTT8780.28194070253971415No Hit
ACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAAAAAAAAA8530.27391277820771776No Hit
TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8520.2735916612344379No Hit
GTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAA8240.2646003859826019No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG7690.2469389524522098No Hit
TCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCTT7100.2279930510286982RNA PCR Primer, Index 46 (95% over 21bp)
TATCAACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAAAA7030.22574523221573925No Hit
CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6850.2199651266967018No Hit
GGTATCAACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAA6720.21579060604406367No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGT6440.20679933079222768No Hit
GAGTACATGGGAATGGTATCAACGCAAAAAAAAAAAAAAAAAA6420.206157096845668No Hit
ACGCAGAGTACATGGGAATGGTATCAACGCAAAAAAAAAAAAA6250.20069810829991042No Hit
GTACATGGGAAGCATGGTATCAACGCAAAAAAAAAAAAAAAAA5600.17982550503671973No Hit
GTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5560.17854103714360028No Hit
GTACATGGGAAGAGTGGTATCAACGCAAAAAAAAAAAAAAAAA5490.1762932183306413No Hit
GTACATGGGAACATGGTATCAACGCAAAAAAAAAAAAAAAAAA5470.17565098438408158No Hit
GTATCAACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAAA5420.1740453995176823No Hit
GAGTACATGGGAACAGTGGTATCAACGCAAAAAAAAAAAAAAA5280.1695497618917643No Hit
GTACATGGGAAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAA4750.1525305623079319No Hit
ATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATG4730.1518883283613722No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGTT4600.14771380770873407No Hit
GAAGCAGAGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAA4410.1416125852164168No Hit
GAACAGTGGTATCAACGCAGAAAAAAAAAAAAAAAAAAAAAAA4390.14097035126985707No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC4380.14064923429657722No Hit
CCCATGTACTCTGCGTTGATACCCTGTCTCTTATACACATCTC4370.14032811732329734No Hit
GAACATGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAA4320.13872253245689808No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATATGCCCA4290.13775918153705852No Hit
ACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCC4280.13743806456377863No Hit
GTACATGGGAATGTATCAACGCAAAAAAAAAAAAAAAAAAAAA4260.13679583061721895No Hit
CCACTGTTCCCATGTACTCTGCGTTGATACCCTGTCTCTTATA4160.13358466088442036No Hit
GAAGAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA4110.13197907601802109No Hit
GGTATCAACGCAGAGTACATGGGAACAGTGGCTGTCTCTTATA3970.12748343839210308No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCC3830.1229878007661851No Hit
CATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTC3820.12266668379290525No Hit
GAACTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAA3810.12234556681962538No Hit
GTACATGGGAAGATGGTATCAACGCAAAAAAAAAAAAAAAAAA3800.12202444984634553No Hit
GGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3670.11784992919370739No Hit
GAATGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3570.11463875946090883No Hit
CAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA3410.10950088788843113No Hit
GGTATCAACGCAGAGTACATGGGCTGTCTCTTATACACATCTC3240.10404189934267356No Hit
CCCATGTACTCTGCGTTGATACCTGTCTCTTATACACATCTCC3210.103078548422834No Hit
CATGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3180.10211519750299443No Hit
GTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3140.10083072960987499No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTG3120.10018849566331527No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCATCT200.001839876537.016
CCGTCTT501.7826096E-1036.99999637
GCCGTCT554.965841E-1033.63636436
ATCTCAT250.00549026829.59999819
TCCTCCC250.00549026829.59999821
CCATCTC250.00549026829.59999817
ATTGACT453.9959305E-628.77777731
ACAACTG551.8968894E-523.5454543
TTTATCC400.001928044323.12537
GGAGCTC502.6959766E-422.1999998
CAGTCGG3400.021.76470610
ATACAAC603.7150672E-521.5833341
AGTCGGT3400.021.22058711
TGGGATG705.0822364E-621.1428596
GGCAGTC3600.020.5555558
CGGGAAC450.00381964120.5555554
GGAAACG450.00381964120.5555558
GCAGTCG3650.020.2739739
TATTGAC555.131529E-420.18181830
CAACTGC656.881497E-519.9230774
Produced by FastQC (version 0.11.5)