FastQCFastQC Report
Fri 10 Feb 2017
ERR1630484.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630484.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences356401
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT24640.6913560848594701No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT17670.4957898546861541No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT14060.39449945426640215No Hit
CTTATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGT13630.3824343927205591No Hit
GGATTACTCCGGTCTGAACTCAGATCACGTAGGACTTTAATCG12050.3381023061102522No Hit
TCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCTT10970.3077993608323209RNA PCR Primer, Index 27 (95% over 23bp)
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10030.2814245751274547No Hit
GAGTAATCCAGGTCGGTTTCTATCTACTTCAAATTCCTCCCTG8700.2441070591833356No Hit
CTCTAATACTGGTGATGCTAGAGGTGATGTTTTTGGTAAACAG8420.2362507400372053No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCC7820.21941577043835453No Hit
CTACTATACTCAATTGATCCAATAACTTGACCAACGGAACAAG7430.2084730401991016No Hit
GATCAGGACATCCCGATGGTGCAGCCGCTATTAAAGGTTCGTT7400.20763129171915903No Hit
GTCCTGATCCAACATCGAGGTCGTAAACCCTATTGTTGATATG7050.1978108927864961No Hit
ACGTAGGACTTTAATCGTTGAACAAACGAACCTTTAATAGCGG6700.18799049385383318No Hit
GTTATCCCTAGGGTAACTTGTTCCGTTGGTCAAGTTATTGGAT6600.18518466558735805No Hit
GTCCTAAACTACTAAACCTGCATTAAAAATTTCGGTTGGGGCG6330.17760892926787522No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTC6310.1770477636145802No Hit
GATAGAAACCGACCTGGATTACTCCGGTCTGAACTCAGATCAC6300.1767671807879327No Hit
AGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCC6240.17508368382804762No Hit
ATTCTAGAGTCCATATCAACAATAGGGTTTACGACCTCGATGT6060.17003319294839242No Hit
TGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTCAATTG5930.16638561620197476No Hit
ATCCTATTCTAGAGTCCATATCAACAATAGGGTTTACGACCTC5910.1658244505486797No Hit
AGGGATAACAGCGCAATCCTATTCTAGAGTCCATATCAACAAT5820.1632992051088521No Hit
CTATTAAAGGTTCGTTTGTTCAACGATTAAAGTCCTACGTGAT5720.160493376842377No Hit
CATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTC5610.15740696574925434RNA PCR Primer, Index 27 (95% over 21bp)
ACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCC5520.15488172030942673No Hit
GTCCTACGTGATCTGAGTTCAGACCGGAGTAATCCAGGTCGGT5410.1517953092163041No Hit
GTTTACGACCTCGATGTTGGATCAGGACATCCCGATGGTGCAG5400.1515147263896566No Hit
GGTCTGAACTCAGATCACGTAGGACTTTAATCGTTGAACAAAC5240.1470254011632964No Hit
ATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATG5180.1453419042034113No Hit
GTCTTAGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGT5100.14309724159023124No Hit
CCATCGGGATGTCCTGATCCAACATCGAGGTCGTAAACCCTAT5020.1408525789770511No Hit
GGACATCCCGATGGTGCAGCCGCTATTAAAGGTTCGTTTGTTC4950.13888849919051854No Hit
GTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAA4940.13860791636387104No Hit
TCCCGATGGTGCAGCCGCTATTAAAGGTTCGTTTGTTCAACGA4860.13636325375069092No Hit
CCTCTAGCATCACCAGTATTAGAGGCACCGCCTGCCCAGTGAC4680.13131276287103572No Hit
TTCGTACAGGGAGGAATTTGAAGTAGATAGAAACCGACCTGGA4670.1310321800443882No Hit
GTACGGGGGAAGGCGCTTTGTGAAGTAGGCCTTATTTCTCTTG4660.13075159721774068No Hit
CCTTTAATAGCGGCTGCACCATCGGGATGTCCTGATCCAACAT4630.12990984873779815No Hit
ACTATACTCAATTGATCCAATAACTTGACCAACGGAACAAGTT4600.12906810025785562No Hit
CTCTAGAATAGGATTGCGCTGTTATCCCTAGGGTAACTTGTTC4600.12906810025785562No Hit
ATACTCAATTGATCCAATAACTTGACCAACGGAACAAGTTACC4570.12822635177791308No Hit
GTGCAGCCGCTATTAAAGGTTCGTTTGTTCAACGATTAAAGTC4550.12766518612461808No Hit
CTACTAAACCTGCATTAAAAATTTCGGTTGGGGCGACCTCGGA4540.12738460329797055No Hit
GAATAGGATTGCGCTGTTATCCCTAGGGTAACTTGTTCCGTTG4490.12598168916473299No Hit
CCATAGGGTCTTCTCGTCTTGCTGTGTCATGCCCGCCTCTTCA4490.12598168916473299No Hit
CGGCTGCACCATCGGGATGTCCTGATCCAACATCGAGGTCGTA4430.1242981922048479No Hit
GTATAGTAGTTCGCTTTGACTGGTGAAGTCTTAGCATGTACTG4420.1240176093782004No Hit
ACAATAGGGTTTACGACCTCGATGTTGGATCAGGACATCCCGA4380.12289527807161035No Hit
CTGTTATCCCTAGGGTAACTTGTTCCGTTGGTCAAGTTATTGG4300.12065061545843025No Hit
ATCTTACCCCGCCTGTTTACCAAAAACATCACCTCTAGCATCA4260.1195282841518402No Hit
GTTCTGCTCCGAGGTCGCCCCAACCGAAATTTTTAATGCAGGT4090.11475837609883248No Hit
CCCATAGTAGGCCTAAAAGCAGCCACCAATTAAGAAAGCGTTC4030.11307487913894741No Hit
GTTCAGACCGGAGTAATCCAGGTCGGTTTCTATCTACTTCAAA3950.11083021652576733No Hit
CTTTCGTACAGGGAGGAATTTGAAGTAGATAGAAACCGACCTG3950.11083021652576733No Hit
GTCGTAAACCCTATTGTTGATATGGACTCTAGAATAGGATTGC3840.1077438054326447No Hit
GTTTAGGACCTGTGGGTTTGTTAGGTACTGTTTGCATTAATAA3830.10746322260599718No Hit
GGGATGTCCTGATCCAACATCGAGGTCGTAAACCCTATTGTTG3760.10549914281946458No Hit
GAGTACGGGGGAAGGCGCTTTGTGAAGTAGGCCTTATTTCTCT3680.1032544802062845No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTCTT1200.033.91666837
GTATGTA303.5945402E-430.8333341
GCCGTCT1350.030.1481536
CCAGGAC250.005491318629.63
CAGCACA250.005491318629.630
TCGGCAG250.005491318629.626
TTATAGA250.005491318629.64
TTGGTGT250.005491318629.622
AGACACC250.005491318629.615
TATAGAT250.005491318629.65
CGTATTA556.241389E-726.9090915
CCGTATT556.241389E-726.9090914
CCGCTTA358.857343E-426.42857225
GTACTGG358.857343E-426.4285721
TCCGTAT601.3330846E-624.66666813
ACTCGCA601.3330846E-624.66666821
TGCCGAG1150.024.13043611
GGAGTTG400.001928587923.1258
GAGTTGA400.001928587923.1259
CCTTTCC1054.0017767E-1122.9047637
Produced by FastQC (version 0.11.5)