##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1630484.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	356401
Sequences flagged as poor quality	0
Sequence length	43
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.921310546266707	33.0	31.0	34.0	30.0	34.0
2	32.0829487010418	33.0	31.0	34.0	30.0	34.0
3	32.1631364670694	34.0	31.0	34.0	30.0	34.0
4	35.76982668398798	37.0	35.0	37.0	35.0	37.0
5	35.76579190294079	37.0	35.0	37.0	35.0	37.0
6	35.826641900555835	37.0	35.0	37.0	35.0	37.0
7	35.80006228938752	37.0	35.0	37.0	35.0	37.0
8	35.80412232288911	37.0	35.0	37.0	35.0	37.0
9	37.512616406800205	39.0	37.0	39.0	35.0	39.0
10	37.40024017889961	39.0	37.0	39.0	34.0	39.0
11	37.52104792074096	39.0	37.0	39.0	35.0	39.0
12	37.45608738471553	39.0	37.0	39.0	35.0	39.0
13	37.50511362201565	39.0	37.0	39.0	35.0	39.0
14	38.74023641908973	40.0	38.0	41.0	35.0	41.0
15	38.696619818687374	40.0	38.0	41.0	35.0	41.0
16	38.64319404266543	40.0	38.0	41.0	34.0	41.0
17	38.63536578180196	40.0	38.0	41.0	34.0	41.0
18	38.6806490441946	40.0	38.0	41.0	35.0	41.0
19	38.74644571704344	40.0	38.0	41.0	35.0	41.0
20	38.68132805463509	40.0	38.0	41.0	34.0	41.0
21	38.60411446656996	40.0	38.0	41.0	34.0	41.0
22	38.556325599535356	40.0	38.0	41.0	34.0	41.0
23	38.54720946349758	40.0	38.0	41.0	34.0	41.0
24	38.5248357889007	40.0	38.0	41.0	34.0	41.0
25	38.424555486656885	40.0	38.0	41.0	34.0	41.0
26	38.224881523901445	40.0	38.0	41.0	34.0	41.0
27	38.109460972331725	40.0	38.0	41.0	33.0	41.0
28	38.028030224382086	40.0	37.0	41.0	33.0	41.0
29	37.946846389319894	40.0	37.0	41.0	33.0	41.0
30	37.81454597489906	40.0	37.0	41.0	33.0	41.0
31	37.7372931052382	40.0	37.0	41.0	33.0	41.0
32	37.641611555523134	40.0	37.0	41.0	33.0	41.0
33	37.617762015258094	40.0	37.0	41.0	33.0	41.0
34	37.5435254109837	40.0	37.0	41.0	32.0	41.0
35	37.44651951032685	40.0	37.0	41.0	32.0	41.0
36	37.42894099623739	40.0	37.0	41.0	32.0	41.0
37	37.218537546190944	40.0	36.0	41.0	31.0	41.0
38	37.09097898154046	40.0	36.0	41.0	31.0	41.0
39	37.00668628875901	39.0	36.0	41.0	31.0	41.0
40	36.87525848692905	39.0	36.0	41.0	31.0	41.0
41	36.76044399426489	39.0	35.0	41.0	30.0	41.0
42	36.63982143708912	39.0	35.0	41.0	30.0	41.0
43	35.78662517781937	38.0	35.0	40.0	28.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	2.0
11	0.0
12	0.0
13	1.0
14	2.0
15	1.0
16	1.0
17	1.0
18	5.0
19	14.0
20	39.0
21	84.0
22	184.0
23	308.0
24	574.0
25	893.0
26	1346.0
27	2022.0
28	2951.0
29	4196.0
30	5559.0
31	7395.0
32	9352.0
33	11987.0
34	16026.0
35	20909.0
36	29034.0
37	45324.0
38	80998.0
39	117192.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.368284600772725	20.4477540747641	12.287563727374502	23.896397597088672
2	19.874242777096583	20.40847247903345	35.113257257976265	24.6040274858937
3	19.989281736022065	24.778269421241802	30.260016105454252	24.97243273728188
4	13.133801532543398	16.676159718968243	36.49428593073532	33.69575281775304
5	12.45815808597619	38.3579170653281	36.3607285052511	12.823196343444604
6	32.15731717924473	36.791984309808335	15.612750805974168	15.437947704972768
7	28.11327689877413	30.979149890151824	22.288096834745133	18.61947637632891
8	26.932584364241404	32.946035504950885	19.934006919172507	20.187373211635208
9	25.947738642708636	13.118650059904432	21.458974582001733	39.474636715385195
10	19.4101587818216	26.234213708715743	32.26309690489084	22.092530604571817
11	33.6825654249006	22.82821877604159	21.37227448856765	22.11694131049015
12	22.306054135650573	28.304914969374384	26.49178874357816	22.89724215139688
13	28.375621841689558	20.35516174197042	26.727478317962074	24.54173809837795
14	22.77350512484533	21.706448635104838	25.688760693712982	29.83128554633685
15	26.93062028445487	26.221587481516607	24.951950190936614	21.89584204309191
16	25.760309314508095	25.493194463539666	25.664630570621295	23.081865651330943
17	22.812506137749335	27.59588216643612	25.63264412838348	23.958967567431067
18	21.44690952045589	25.349816639122785	28.78920092816799	24.41407291225333
19	23.670528421637425	24.77406067884209	28.92893117583845	22.62647972368203
20	23.184278383057286	24.153130883471146	30.077637268133365	22.584953465338202
21	23.34841933664608	25.79117342543932	28.392176228461764	22.468231009452836
22	24.45980791299688	25.847009407942178	27.0347165131411	22.65846616591985
23	23.1205860814083	25.584103299373457	28.984767158341306	22.310543460876932
24	23.25947458059882	26.86496390301935	26.00553870499802	23.870022811383805
25	23.290338691530046	25.455876947595545	28.61103083324682	22.642753527627587
26	22.971316017631825	26.588589818771553	28.216811961807064	22.22328220178956
27	23.197746358736367	26.31389923148364	26.80099101854372	23.68736339123628
28	22.01537032724375	25.187639765320526	30.311082179904098	22.48590772753163
29	23.906779161674628	25.762273394294628	28.595598777781206	21.735348666249536
30	22.263686128826798	25.645270355582618	29.78526996276666	22.305773552823926
31	22.568679661392647	26.986456266957724	28.143579844052063	22.301284227597566
32	21.431758047816928	25.758625817548207	28.781064026195214	24.02855210843965
33	21.2314219095906	25.49375562919296	30.277131657879746	22.99769080333669
34	23.059419025199144	24.984217216001078	29.635999898990185	22.320363859809596
35	21.809422532484476	26.53920724128159	28.70474549734709	22.946624728886846
36	22.902012059449888	26.42192361974293	27.607386062328672	23.06867825847851
37	21.960656676047485	24.89022196907416	29.34952483298307	23.799596521895282
38	22.12928695486264	24.47972929368885	29.79172336777955	23.59926038366896
39	21.323733659557632	26.02040959481034	29.46708903734838	23.18876770828365
40	21.05802172272244	25.71120731984478	30.058277053094688	23.17249390433809
41	21.525472711917196	24.596451749574218	29.38684234892719	24.4912331895814
42	20.532209505585	26.05997177336764	30.147782974795245	23.260035746252115
43	21.924461491409957	23.77939455837666	29.784147631460065	24.511996318753315
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	15.0
1	22.0
2	29.0
3	126.5
4	224.0
5	224.0
6	327.5
7	431.0
8	447.0
9	463.0
10	647.5
11	832.0
12	832.0
13	1616.5
14	2401.0
15	3209.0
16	4017.0
17	3584.0
18	3151.0
19	3151.0
20	3674.5
21	4198.0
22	2865.5
23	1533.0
24	1506.5
25	1480.0
26	1480.0
27	1873.5
28	2267.0
29	3198.0
30	4129.0
31	5791.0
32	7453.0
33	7453.0
34	10663.5
35	13874.0
36	16086.5
37	18299.0
38	22284.0
39	26269.0
40	26269.0
41	27901.5
42	29534.0
43	32229.5
44	34925.0
45	33051.5
46	31178.0
47	31178.0
48	31826.5
49	32475.0
50	31654.5
51	30834.0
52	28906.0
53	26978.0
54	26978.0
55	24008.0
56	21038.0
57	18221.5
58	15405.0
59	14259.0
60	13113.0
61	13113.0
62	11392.5
63	9672.0
64	8254.5
65	6837.0
66	5924.5
67	5012.0
68	5012.0
69	4075.0
70	3138.0
71	2623.0
72	2108.0
73	1652.0
74	1196.0
75	1196.0
76	972.5
77	749.0
78	604.5
79	460.0
80	386.0
81	312.0
82	312.0
83	249.5
84	187.0
85	142.5
86	98.0
87	74.5
88	51.0
89	51.0
90	39.5
91	28.0
92	17.0
93	6.0
94	4.0
95	2.0
96	2.0
97	1.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	356401.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	28.51725370458808
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.54687676859623	21.543894513993102
2	9.792025045208701	5.584833249917943
3	3.636461340386591	3.1110567189219256
4	1.9852397744146308	2.2645434524568495
5	1.3414664452041023	1.9127469477038643
6	0.9936038770878922	1.7000912306866665
7	0.7297090418925855	1.4566508514727925
8	0.5987612527047218	1.3660021241486022
9	0.45881705330100664	1.1775782081678687
>10	4.032392141584743	23.502819095549064
>50	0.4978015088024257	9.877860098957209
>100	0.3548585052708054	19.082696196597382
>500	0.024990035582451088	4.481695615716416
>1k	0.006997209963086305	2.937531695710321
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	2464	0.6913560848594701	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	1767	0.4957898546861541	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	1406	0.39449945426640215	No Hit
CTTATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGT	1363	0.3824343927205591	No Hit
GGATTACTCCGGTCTGAACTCAGATCACGTAGGACTTTAATCG	1205	0.3381023061102522	No Hit
TCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCTT	1097	0.3077993608323209	RNA PCR Primer, Index 27 (95% over 23bp)
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1003	0.2814245751274547	No Hit
GAGTAATCCAGGTCGGTTTCTATCTACTTCAAATTCCTCCCTG	870	0.2441070591833356	No Hit
CTCTAATACTGGTGATGCTAGAGGTGATGTTTTTGGTAAACAG	842	0.2362507400372053	No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCC	782	0.21941577043835453	No Hit
CTACTATACTCAATTGATCCAATAACTTGACCAACGGAACAAG	743	0.2084730401991016	No Hit
GATCAGGACATCCCGATGGTGCAGCCGCTATTAAAGGTTCGTT	740	0.20763129171915903	No Hit
GTCCTGATCCAACATCGAGGTCGTAAACCCTATTGTTGATATG	705	0.1978108927864961	No Hit
ACGTAGGACTTTAATCGTTGAACAAACGAACCTTTAATAGCGG	670	0.18799049385383318	No Hit
GTTATCCCTAGGGTAACTTGTTCCGTTGGTCAAGTTATTGGAT	660	0.18518466558735805	No Hit
GTCCTAAACTACTAAACCTGCATTAAAAATTTCGGTTGGGGCG	633	0.17760892926787522	No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTC	631	0.1770477636145802	No Hit
GATAGAAACCGACCTGGATTACTCCGGTCTGAACTCAGATCAC	630	0.1767671807879327	No Hit
AGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCC	624	0.17508368382804762	No Hit
ATTCTAGAGTCCATATCAACAATAGGGTTTACGACCTCGATGT	606	0.17003319294839242	No Hit
TGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTCAATTG	593	0.16638561620197476	No Hit
ATCCTATTCTAGAGTCCATATCAACAATAGGGTTTACGACCTC	591	0.1658244505486797	No Hit
AGGGATAACAGCGCAATCCTATTCTAGAGTCCATATCAACAAT	582	0.1632992051088521	No Hit
CTATTAAAGGTTCGTTTGTTCAACGATTAAAGTCCTACGTGAT	572	0.160493376842377	No Hit
CATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTC	561	0.15740696574925434	RNA PCR Primer, Index 27 (95% over 21bp)
ACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCC	552	0.15488172030942673	No Hit
GTCCTACGTGATCTGAGTTCAGACCGGAGTAATCCAGGTCGGT	541	0.1517953092163041	No Hit
GTTTACGACCTCGATGTTGGATCAGGACATCCCGATGGTGCAG	540	0.1515147263896566	No Hit
GGTCTGAACTCAGATCACGTAGGACTTTAATCGTTGAACAAAC	524	0.1470254011632964	No Hit
ATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATG	518	0.1453419042034113	No Hit
GTCTTAGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGT	510	0.14309724159023124	No Hit
CCATCGGGATGTCCTGATCCAACATCGAGGTCGTAAACCCTAT	502	0.1408525789770511	No Hit
GGACATCCCGATGGTGCAGCCGCTATTAAAGGTTCGTTTGTTC	495	0.13888849919051854	No Hit
GTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAA	494	0.13860791636387104	No Hit
TCCCGATGGTGCAGCCGCTATTAAAGGTTCGTTTGTTCAACGA	486	0.13636325375069092	No Hit
CCTCTAGCATCACCAGTATTAGAGGCACCGCCTGCCCAGTGAC	468	0.13131276287103572	No Hit
TTCGTACAGGGAGGAATTTGAAGTAGATAGAAACCGACCTGGA	467	0.1310321800443882	No Hit
GTACGGGGGAAGGCGCTTTGTGAAGTAGGCCTTATTTCTCTTG	466	0.13075159721774068	No Hit
CCTTTAATAGCGGCTGCACCATCGGGATGTCCTGATCCAACAT	463	0.12990984873779815	No Hit
ACTATACTCAATTGATCCAATAACTTGACCAACGGAACAAGTT	460	0.12906810025785562	No Hit
CTCTAGAATAGGATTGCGCTGTTATCCCTAGGGTAACTTGTTC	460	0.12906810025785562	No Hit
ATACTCAATTGATCCAATAACTTGACCAACGGAACAAGTTACC	457	0.12822635177791308	No Hit
GTGCAGCCGCTATTAAAGGTTCGTTTGTTCAACGATTAAAGTC	455	0.12766518612461808	No Hit
CTACTAAACCTGCATTAAAAATTTCGGTTGGGGCGACCTCGGA	454	0.12738460329797055	No Hit
GAATAGGATTGCGCTGTTATCCCTAGGGTAACTTGTTCCGTTG	449	0.12598168916473299	No Hit
CCATAGGGTCTTCTCGTCTTGCTGTGTCATGCCCGCCTCTTCA	449	0.12598168916473299	No Hit
CGGCTGCACCATCGGGATGTCCTGATCCAACATCGAGGTCGTA	443	0.1242981922048479	No Hit
GTATAGTAGTTCGCTTTGACTGGTGAAGTCTTAGCATGTACTG	442	0.1240176093782004	No Hit
ACAATAGGGTTTACGACCTCGATGTTGGATCAGGACATCCCGA	438	0.12289527807161035	No Hit
CTGTTATCCCTAGGGTAACTTGTTCCGTTGGTCAAGTTATTGG	430	0.12065061545843025	No Hit
ATCTTACCCCGCCTGTTTACCAAAAACATCACCTCTAGCATCA	426	0.1195282841518402	No Hit
GTTCTGCTCCGAGGTCGCCCCAACCGAAATTTTTAATGCAGGT	409	0.11475837609883248	No Hit
CCCATAGTAGGCCTAAAAGCAGCCACCAATTAAGAAAGCGTTC	403	0.11307487913894741	No Hit
GTTCAGACCGGAGTAATCCAGGTCGGTTTCTATCTACTTCAAA	395	0.11083021652576733	No Hit
CTTTCGTACAGGGAGGAATTTGAAGTAGATAGAAACCGACCTG	395	0.11083021652576733	No Hit
GTCGTAAACCCTATTGTTGATATGGACTCTAGAATAGGATTGC	384	0.1077438054326447	No Hit
GTTTAGGACCTGTGGGTTTGTTAGGTACTGTTTGCATTAATAA	383	0.10746322260599718	No Hit
GGGATGTCCTGATCCAACATCGAGGTCGTAAACCCTATTGTTG	376	0.10549914281946458	No Hit
GAGTACGGGGGAAGGCGCTTTGTGAAGTAGGCCTTATTTCTCT	368	0.1032544802062845	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	5.611656532950244E-4	2.805828266475122E-4
2	0.0	0.0	0.0	0.00252524543982761	2.805828266475122E-4
3	0.0	0.0	0.0	0.004208742399712683	2.805828266475122E-4
4	0.0	0.0	0.0	0.005892239359597757	2.805828266475122E-4
5	0.0	0.0	0.0	0.005892239359597757	2.805828266475122E-4
6	0.0	0.0	0.0	0.005892239359597757	2.805828266475122E-4
7	0.0	0.0	0.0	0.006172822186245269	2.805828266475122E-4
8	0.0	0.0	0.0	0.0067339878395402935	2.805828266475122E-4
9	0.0	0.0	0.0	0.00897865045272039	2.805828266475122E-4
10	0.0	0.0	0.0	0.011223313065900489	2.805828266475122E-4
11	0.0	0.0	0.0	0.012065061545843025	2.805828266475122E-4
12	0.0	0.0	0.0	0.012065061545843025	2.805828266475122E-4
13	0.0	0.0	0.0	0.012906810025785562	2.805828266475122E-4
14	0.0	0.0	0.0	0.013748558505728098	2.805828266475122E-4
15	0.0	0.0	0.0	0.015151472638965659	2.805828266475122E-4
16	0.0	0.0	0.0	0.01907963221203083	2.805828266475122E-4
17	0.0	0.0	0.0	0.028338865491398732	2.805828266475122E-4
18	0.0	0.0	0.0	0.031144693757873855	2.805828266475122E-4
19	0.0	0.0	0.0	0.036475767464176585	2.805828266475122E-4
20	0.0	0.0	0.0	0.0390010129040042	2.805828266475122E-4
21	0.0	0.0	0.0	0.0482602461833721	2.805828266475122E-4
22	0.0	0.0	0.0	0.06004472490256761	2.805828266475122E-4
23	0.0	0.0	0.0	0.07687969450141835	2.805828266475122E-4
24	0.0	0.0	0.0	0.1032544802062845	2.805828266475122E-4
25	0.0	0.0	0.0	0.11167196500570986	2.805828266475122E-4
26	0.0	0.0	0.0	0.12317586089825786	2.805828266475122E-4
27	0.0	0.0	0.0	0.1425360759369362	2.805828266475122E-4
28	0.0	0.0	0.0	0.16863027881515483	2.805828266475122E-4
29	0.0	0.0	0.0	0.20538662910597894	2.805828266475122E-4
30	0.0	0.0	0.0	0.26992067923490676	2.805828266475122E-4
31	0.0	0.0	0.0	0.5100995788451772	2.805828266475122E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CCGTCTT	120	0.0	33.916668	37
GTATGTA	30	3.5945402E-4	30.833334	1
GCCGTCT	135	0.0	30.14815	36
CCAGGAC	25	0.0054913186	29.6	3
CAGCACA	25	0.0054913186	29.6	30
TCGGCAG	25	0.0054913186	29.6	26
TTATAGA	25	0.0054913186	29.6	4
TTGGTGT	25	0.0054913186	29.6	22
AGACACC	25	0.0054913186	29.6	15
TATAGAT	25	0.0054913186	29.6	5
CGTATTA	55	6.241389E-7	26.90909	15
CCGTATT	55	6.241389E-7	26.90909	14
CCGCTTA	35	8.857343E-4	26.428572	25
GTACTGG	35	8.857343E-4	26.428572	1
TCCGTAT	60	1.3330846E-6	24.666668	13
ACTCGCA	60	1.3330846E-6	24.666668	21
TGCCGAG	115	0.0	24.130436	11
GGAGTTG	40	0.0019285879	23.125	8
GAGTTGA	40	0.0019285879	23.125	9
CCTTTCC	105	4.0017767E-11	22.90476	37
>>END_MODULE