FastQCFastQC Report
Fri 10 Feb 2017
ERR1630483.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630483.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1965403
Sequences flagged as poor quality0
Sequence length43
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT82190.41818395514812995No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT77770.39569492872454143No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT59360.302024572059776No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT45160.22977475866272717No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGCGAA502.703048E-422.211
AACCGTC555.1448797E-420.1818187
TAAGACG701.21975645E-418.54
CTAGCAC3400.017.4117643
TACACCG752.0682078E-417.2666665
GGTATCA39150.016.397191
CCTATAC2250.015.6222223
AAGACGG3900.015.1794875
ACCTTAG1406.0005914E-714.5357141
TAACGGC1152.2113121E-514.47826136
TACCGTC2700.014.3888897
ACACCGT908.277702E-414.3888886
ATCTCGC2850.014.28070211
TCGCCAT2600.014.23076913
CGCAAGA3800.014.1184222
GAACCGT1051.6565748E-414.0952396
CGTCGTA2500.014.05999910
CGAACGA1854.9039954E-914.016
ACCGTCG2650.013.9622648
AAATCGG1203.3027027E-513.87500126
Produced by FastQC (version 0.11.5)