##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1630483.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1965403
Sequences flagged as poor quality	0
Sequence length	43
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.92880900253027	33.0	31.0	34.0	30.0	34.0
2	32.09434808026649	33.0	31.0	34.0	30.0	34.0
3	32.196154172960966	34.0	31.0	34.0	30.0	34.0
4	35.79235556270139	37.0	35.0	37.0	35.0	37.0
5	35.77358333125573	37.0	35.0	37.0	35.0	37.0
6	35.808479991126504	37.0	35.0	37.0	35.0	37.0
7	35.78218207665298	37.0	35.0	37.0	35.0	37.0
8	35.77507819007094	37.0	35.0	37.0	35.0	37.0
9	37.4922939468394	39.0	37.0	39.0	35.0	39.0
10	37.38755563108431	39.0	37.0	39.0	34.0	39.0
11	37.50443293309311	39.0	37.0	39.0	35.0	39.0
12	37.430203373048684	39.0	37.0	39.0	34.0	39.0
13	37.48439022429497	39.0	37.0	39.0	35.0	39.0
14	38.74241058958392	40.0	38.0	41.0	35.0	41.0
15	38.713557473963355	40.0	38.0	41.0	35.0	41.0
16	38.69139458930306	40.0	38.0	41.0	34.0	41.0
17	38.683160145781805	40.0	38.0	41.0	34.0	41.0
18	38.67317644269394	40.0	38.0	41.0	34.0	41.0
19	38.705495005350045	40.0	38.0	41.0	34.0	41.0
20	38.68952474378028	40.0	38.0	41.0	34.0	41.0
21	38.61649595528245	40.0	38.0	41.0	34.0	41.0
22	38.56169141901177	40.0	38.0	41.0	34.0	41.0
23	38.53498493693151	40.0	38.0	41.0	34.0	41.0
24	38.48497687242769	40.0	38.0	41.0	34.0	41.0
25	38.44578185746129	40.0	38.0	41.0	34.0	41.0
26	38.24805548785669	40.0	38.0	41.0	34.0	41.0
27	38.13183352218349	40.0	38.0	41.0	33.0	41.0
28	38.041862152444054	40.0	37.0	41.0	33.0	41.0
29	37.93634129997766	40.0	37.0	41.0	33.0	41.0
30	37.85152510706455	40.0	37.0	41.0	33.0	41.0
31	37.789704706871824	40.0	37.0	41.0	33.0	41.0
32	37.68949421569012	40.0	37.0	41.0	33.0	41.0
33	37.65129390766169	40.0	37.0	41.0	33.0	41.0
34	37.56916571308785	40.0	37.0	41.0	32.0	41.0
35	37.51241857267949	40.0	37.0	41.0	32.0	41.0
36	37.46974132022796	40.0	37.0	41.0	32.0	41.0
37	37.281887226182114	40.0	36.0	41.0	31.0	41.0
38	37.16479266593162	40.0	36.0	41.0	31.0	41.0
39	37.097283356136124	39.0	36.0	41.0	31.0	41.0
40	36.98773177816458	39.0	36.0	41.0	31.0	41.0
41	36.84644472405914	39.0	35.0	41.0	31.0	41.0
42	36.75326281683706	39.0	35.0	41.0	30.0	41.0
43	35.9193524177993	38.0	35.0	40.0	29.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	2.0
9	4.0
10	5.0
11	6.0
12	5.0
13	2.0
14	4.0
15	5.0
16	12.0
17	31.0
18	61.0
19	160.0
20	310.0
21	558.0
22	1068.0
23	1821.0
24	3111.0
25	4886.0
26	7507.0
27	11195.0
28	16131.0
29	22554.0
30	30273.0
31	39493.0
32	50963.0
33	65669.0
34	85878.0
35	114734.0
36	160558.0
37	244735.0
38	441140.0
39	662522.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.28725457323511	19.63587111650893	12.486650320570387	24.590223989685576
2	18.51121627472839	21.218956112308774	35.317947515089784	24.951880097873058
3	20.11444981003896	23.834094076380264	29.706833662103904	26.344622451476873
4	14.300527678038549	15.80530812255807	34.510225129400936	35.383939070002434
5	14.013105709109022	37.61778118787852	34.19258035120533	14.176532751807136
6	34.327718030347974	34.88424511410637	16.042155222109663	14.745881633435992
7	28.64786509433434	31.023968112392218	21.7112724464143	18.61689434685914
8	26.824066107561656	33.91329920632054	20.206644642345616	19.055990043772194
9	26.46179943757082	13.969704940920513	20.40288938197408	39.16560623953459
10	16.992596429332814	27.090016653073185	33.17009285118625	22.747294066407754
11	34.76605052500683	22.19463387407061	21.841780031881502	21.197535569041058
12	21.098268395845533	26.368790522859687	29.498530326859175	23.034410754435605
13	29.294144763185976	20.214022264136158	26.212334060749882	24.279498911927984
14	22.572673390648127	21.61495632193499	26.038883628446683	29.773486658970192
15	25.468364503361396	28.004078552846412	23.27476858435649	23.252788359435698
16	24.646090394692592	26.870112643564703	24.8936223258029	23.590174635939803
17	23.08091521179117	27.15214131656459	26.208111008276674	23.558832463367562
18	22.642786237733432	25.54300568382159	27.96281475096965	23.851393327475332
19	23.38660315467108	26.084675763698336	27.964748196680272	22.56397288495031
20	23.96022596892342	25.177686204813977	27.8955511923	22.966536633962605
21	24.200889079745984	25.84457233452885	26.913309891152092	23.04122869457307
22	24.031712580066277	25.641713175364032	27.00056934888163	23.32600489568806
23	23.17789277822411	25.938497091945013	27.378812386060265	23.504797743770617
24	23.498590365436502	26.346606777337776	27.15168339521208	23.003119462013643
25	23.75711240900721	25.625838568476794	27.28132601812453	23.335723004391465
26	23.34193038272558	26.527790992483474	27.21477478155879	22.915503843232152
27	23.761742502682655	25.914278140411916	26.632451461608635	23.69152789529679
28	22.891488412300173	26.13723495893717	27.700018774775454	23.271257853987198
29	22.73477754943897	26.501892995991156	27.6886725012631	23.07465695330678
30	22.581933577999017	27.083656634288232	27.590270290622332	22.74413949709042
31	23.327938341398685	26.55231522491825	27.00148519158666	23.118261242096406
32	22.485312172618034	26.48978352022461	27.309768022130832	23.71513628502653
33	22.510141685954483	26.297863593369907	27.729631022238188	23.462363698437418
34	23.360552517727918	25.91117445124486	27.416412817116896	23.311860213910325
35	23.173059163947546	26.08808473376707	27.694625478845815	23.044230623439567
36	22.64548288569825	26.499348988477173	27.354644314677444	23.50052381114713
37	23.258792217168693	25.512172312752142	27.552059297762344	23.67697617231682
38	22.58686895257614	25.577400665410604	28.30493288144976	23.530797500563498
39	22.76642500291289	25.543412725023824	28.0874711191547	23.60269115290859
40	22.24169801307925	25.391077555086667	28.844923916367282	23.522300515466803
41	21.677742427380036	25.6727500670346	29.049920041843833	23.599587463741535
42	22.27507539166268	25.518634091837654	28.803914515241914	23.402376001257757
43	21.748618476719532	25.140238414208184	28.85118217485167	24.259960934220615
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	539.0
1	482.0
2	425.0
3	1080.5
4	1736.0
5	1736.0
6	2291.0
7	2846.0
8	2781.0
9	2716.0
10	4074.0
11	5432.0
12	5432.0
13	9055.0
14	12678.0
15	16945.0
16	21212.0
17	20488.5
18	19765.0
19	19765.0
20	22787.0
21	25809.0
22	24969.5
23	24130.0
24	26850.5
25	29571.0
26	29571.0
27	33773.5
28	37976.0
29	45110.5
30	52245.0
31	58092.5
32	63940.0
33	63940.0
34	70186.0
35	76432.0
36	83291.0
37	90150.0
38	99068.0
39	107986.0
40	107986.0
41	113557.0
42	119128.0
43	119179.0
44	119230.0
45	123296.0
46	127362.0
47	127362.0
48	131446.0
49	135530.0
50	137191.5
51	138853.0
52	142014.5
53	145176.0
54	145176.0
55	135689.5
56	126203.0
57	117995.5
58	109788.0
59	101943.5
60	94099.0
61	94099.0
62	87619.0
63	81139.0
64	71667.0
65	62195.0
66	53874.0
67	45553.0
68	45553.0
69	38487.5
70	31422.0
71	27115.0
72	22808.0
73	17899.0
74	12990.0
75	12990.0
76	10174.5
77	7359.0
78	5890.0
79	4421.0
80	3515.0
81	2609.0
82	2609.0
83	1993.0
84	1377.0
85	1260.5
86	1144.0
87	1025.0
88	906.0
89	906.0
90	680.0
91	454.0
92	250.5
93	47.0
94	30.5
95	14.0
96	14.0
97	10.0
98	6.0
99	4.0
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1965403.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	56.36958582000757
#Duplication Level	Percentage of deduplicated	Percentage of total
1	86.23072254946163	48.60790115073139
2	8.37610970386717	9.443156695798773
3	2.091160425775963	3.53633541252545
4	0.8930469235866836	2.013627408016532
5	0.4885584850811728	1.376991972643803
6	0.3020650313122143	1.0216368423466282
7	0.2238705630532206	0.8833643641621354
8	0.16076049659846345	0.7249602087579295
9	0.12666677010363486	0.6426138031110012
>10	0.8628642297471751	9.756620228208075
>50	0.12144403626326228	4.817101731192467
>100	0.11412686711354308	12.49401750334548
>500	0.007426539778451928	2.783028066247254
>1k	9.056755827380399E-4	0.7789599221250088
>5k	2.71702674821412E-4	1.1196846907881566
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	8219	0.41818395514812995	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	7777	0.39569492872454143	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	5936	0.302024572059776	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4516	0.22977475866272717	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	5.088015027961187E-5	0.0
2	0.0	5.088015027961187E-5	0.0	1.5264045083883559E-4	0.0
3	0.0	5.088015027961187E-5	0.0	3.5616105195728306E-4	0.0
4	0.0	5.088015027961187E-5	0.0	5.088015027961187E-4	0.0
5	0.0	1.0176030055922374E-4	0.0	5.596816530757306E-4	0.0
6	0.0	1.0176030055922374E-4	0.0	5.596816530757306E-4	0.0
7	0.0	1.0176030055922374E-4	0.0	7.63202254194178E-4	0.0
8	0.0	1.0176030055922374E-4	0.0	8.140824044737899E-4	0.0
9	0.0	1.0176030055922374E-4	0.0	0.0017808052597864154	0.0
10	0.0	1.0176030055922374E-4	0.0	0.0024931273637009816	0.0
11	0.0	1.0176030055922374E-4	0.0	0.0032054494676155474	0.0
12	0.0	1.0176030055922374E-4	0.0	0.003866891421250502	0.0
13	0.0	1.0176030055922374E-4	0.0	0.0042739326234873965	0.0
14	0.0	1.0176030055922374E-4	0.0	0.005342415779359246	0.0
15	0.0	1.0176030055922374E-4	0.0	0.006512659235790319	0.0
16	0.0	1.5264045083883559E-4	0.0	0.008496985096695182	0.0
17	0.0	1.5264045083883559E-4	0.0	0.012211236067106848	0.0
18	0.0	2.0352060111844747E-4	0.0	0.013534119974376757	5.088015027961187E-5
19	0.0	2.0352060111844747E-4	0.0	0.016179887788916575	5.088015027961187E-5
20	0.0	2.0352060111844747E-4	0.0	0.017808052597864152	5.088015027961187E-5
21	0.0	2.0352060111844747E-4	0.0	0.02325222867778262	1.0176030055922374E-4
22	0.0	2.0352060111844747E-4	0.0	0.03266505647951082	1.0176030055922374E-4
23	0.0	2.5440075139805934E-4	0.0	0.04675885810696331	1.0176030055922374E-4
24	0.0	2.5440075139805934E-4	0.0	0.07087604933949933	1.0176030055922374E-4
25	0.0	3.0528090167767117E-4	0.0	0.07703254752333237	1.0176030055922374E-4
26	0.0	3.0528090167767117E-4	0.0	0.085733053221146	1.0176030055922374E-4
27	0.0	3.0528090167767117E-4	0.0	0.10282878371509559	1.0176030055922374E-4
28	0.0	3.0528090167767117E-4	0.0	0.1419556192801171	1.0176030055922374E-4
29	0.0	3.0528090167767117E-4	0.0	0.19288664971000857	1.0176030055922374E-4
30	0.0	3.0528090167767117E-4	0.0	0.27337904745235453	1.0176030055922374E-4
31	0.0	3.0528090167767117E-4	0.0	0.5394822334147246	1.0176030055922374E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCGCGAA	50	2.703048E-4	22.2	11
AACCGTC	55	5.1448797E-4	20.181818	7
TAAGACG	70	1.21975645E-4	18.5	4
CTAGCAC	340	0.0	17.411764	3
TACACCG	75	2.0682078E-4	17.266666	5
GGTATCA	3915	0.0	16.39719	1
CCTATAC	225	0.0	15.622222	3
AAGACGG	390	0.0	15.179487	5
ACCTTAG	140	6.0005914E-7	14.535714	1
TAACGGC	115	2.2113121E-5	14.478261	36
TACCGTC	270	0.0	14.388889	7
ACACCGT	90	8.277702E-4	14.388888	6
ATCTCGC	285	0.0	14.280702	11
TCGCCAT	260	0.0	14.230769	13
CGCAAGA	380	0.0	14.118422	2
GAACCGT	105	1.6565748E-4	14.095239	6
CGTCGTA	250	0.0	14.059999	10
CGAACGA	185	4.9039954E-9	14.0	16
ACCGTCG	265	0.0	13.962264	8
AAATCGG	120	3.3027027E-5	13.875001	26
>>END_MODULE