FastQCFastQC Report
Fri 10 Feb 2017
ERR1630482.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630482.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences712631
Sequences flagged as poor quality0
Sequence length43
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT98211.3781325819393206No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT74941.051596127589173No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT60540.8495280166032632No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT48440.6797346733442693No Hit
GGATTACTCCGGTCTGAACTCAGATCACGTAGGACTTTAATCG19140.268582197518772No Hit
GAGTAATCCAGGTCGGTTTCTATCTACTTCAAATTCCTCCCTG14200.199261609444439No Hit
CTACTATACTCAATTGATCCAATAACTTGACCAACGGAACAAG13920.19533250728637963No Hit
CTTATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGT13880.19477120697808545No Hit
CTCTAATACTGGTGATGCTAGAGGTGATGTTTTTGGTAAACAG13290.18649202743074605No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13240.18579040204537833No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT12590.17666927203559768No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12460.17484504603364154No Hit
GTCCTGATCCAACATCGAGGTCGTAAACCCTATTGTTGATATG11900.16698684171752282No Hit
GTCCTAAACTACTAAACCTGCATTAAAAATTTCGGTTGGGGCG11790.16544326586971378No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTC11430.16039156309506603No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTT11360.15940928755555117No Hit
GATCAGGACATCCCGATGGTGCAGCCGCTATTAAAGGTTCGTT11220.15744473647652152No Hit
AGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCC11130.15618181078285956No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCC10880.15267368385602087No Hit
GATAGAAACCGACCTGGATTACTCCGGTCTGAACTCAGATCAC10850.1522527086248002No Hit
ATTCTAGAGTCCATATCAACAATAGGGTTTACGACCTCGATGT10520.1476219810813731No Hit
ACGTAGGACTTTAATCGTTGAACAAACGAACCTTTAATAGCGG10490.14720100585015244No Hit
GTTATCCCTAGGGTAACTTGTTCCGTTGGTCAAGTTATTGGAT10310.14467515446282858No Hit
AGGGATAACAGCGCAATCCTATTCTAGAGTCCATATCAACAAT10190.142991253537946No Hit
TGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTCAATTG10170.1427106033837989No Hit
CTATTAAAGGTTCGTTTGTTCAACGATTAAAGTCCTACGTGAT9750.1368169501467099No Hit
TCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTT9220.1293797210618118RNA PCR Primer, Index 26 (100% over 22bp)
GTCTTAGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGT9080.12741516998278213No Hit
GTACGGGGGAAGGCGCTTTGTGAAGTAGGCCTTATTTCTCTTG8990.12615224428912017No Hit
TTCGTACAGGGAGGAATTTGAAGTAGATAGAAACCGACCTGGA8840.12404736813301695No Hit
ATCCTATTCTAGAGTCCATATCAACAATAGGGTTTACGACCTC8670.12166184182276663No Hit
GGTCTGAACTCAGATCACGTAGGACTTTAATCGTTGAACAAAC8450.11857469012714855No Hit
GTCCTACGTGATCTGAGTTCAGACCGGAGTAATCCAGGTCGGT8440.11843436505007501No Hit
ACTATACTCAATTGATCCAATAACTTGACCAACGGAACAAGTT8390.11773273966470725No Hit
GTGCAGCCGCTATTAAAGGTTCGTTTGTTCAACGATTAAAGTC8240.11562786350860403No Hit
CTCTAGAATAGGATTGCGCTGTTATCCCTAGGGTAACTTGTTC8160.11450526289201565No Hit
GTTTACGACCTCGATGTTGGATCAGGACATCCCGATGGTGCAG8070.1132423371983537No Hit
CCCATAGTAGGCCTAAAAGCAGCCACCAATTAAGAAAGCGTTC8060.11310201212128017No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8030.11268103689005952No Hit
CTACTAAACCTGCATTAAAAATTTCGGTTGGGGCGACCTCGGA8000.11226006165883887No Hit
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA7950.11155843627347112No Hit
GTTTAGGACCTGTGGGTTTGTTAGGTACTGTTTGCATTAATAA7720.10833095950077949No Hit
ACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAAAAAAAAA7640.10720835888419111No Hit
CTTTCGTACAGGGAGGAATTTGAAGTAGATAGAAACCGACCTG7610.10678738365297047No Hit
TCCCGATGGTGCAGCCGCTATTAAAGGTTCGTTTGTTCAACGA7500.10524380780516143No Hit
GTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAA7490.1051034827280879No Hit
ATACTCAATTGATCCAATAACTTGACCAACGGAACAAGTTACC7310.102577631340764No Hit
CCTTTAATAGCGGCTGCACCATCGGGATGTCCTGATCCAACAT7230.10145503072417562No Hit
ACAATAGGGTTTACGACCTCGATGTTGGATCAGGACATCCCGA7150.10033243010758724No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACCATG200.001841466537.07
TGATACA303.5981188E-430.8333325
CTAGCCT303.5981188E-430.8333324
CATGGAT250.0054949629.5999984
GGCAGAC250.0054949629.5999988
CGTCTTC250.0054949629.59999837
AGCCTTA250.0054949629.5999986
CCGTCTT1350.027.40740837
TTAGGTT358.866073E-426.4285724
CTGTCTG358.866073E-426.4285729
GCCGTCT1400.026.42857236
AACGCTC451.3225232E-424.6666667
TGAAGTT551.9013441E-523.5454544
GCAGCGT551.9013441E-523.5454541
CATTACA400.001930470723.12500224
TTAGTCA400.001930470723.12500236
ACGCTCC502.7007068E-422.1999998
ACATCCG855.1737516E-821.76470610
AGCGTCA603.7237296E-521.5833323
TCATTTG450.003824403520.55555512
Produced by FastQC (version 0.11.5)