FastQCFastQC Report
Fri 10 Feb 2017
ERR1630481.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630481.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2227761
Sequences flagged as poor quality0
Sequence length43
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT67130.3013339402206969No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT64080.287643064045021No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT49030.22008644553881676No Hit
GCTTAGGACACAGAGCACATCAAAAGTTCCCAAAGAGGGCTTG36120.1621358844148901No Hit
GTTCTACAGCACACTACCAGAAGACAGCAGAAATGAAAAGCAT35970.1614625626357585No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32380.1453477280552088No Hit
CTGTAGAACAGAGCAGGTGAAGAGAGAGCAAGCCCTCTTTGGG31010.13919805580580683No Hit
TCCCAGAAGAGGTCGCCATTGTTGAAGAACTTGGCCGCAGACA29780.13367681721692767No Hit
CTTCTGGGAAATCTCGCCTTCCTCGGCCTTTCACCAGCCAAGC29100.13062442515153105No Hit
ATTGAAAGCTGAGTATTTTTAAGACAAAGGTTTCAGGAAGAAA23650.10616040050974948No Hit
GAATACACCTCTTAAATTTACAGGACTTAACATTTCAAACATC22460.10081871439530542No Hit
CTTCAAGACACAGAGGAGAAATCCAGATCATTCTCAGCTTCCC22280.1000107282603475No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAATTA2300.020.10869615
AGTACCG609.2405255E-418.55
CTTATAC12350.018.42510237
GGTATCA31450.017.5294111
TACGTTA1951.8189894E-1216.12820619
CGAACGA1851.8189894E-1116.016
CGGCCTT8100.015.98765524
CCCGAAT2900.015.94827613
TAGAACA10850.015.8571434
TCTTATA20650.015.76755537
TAGACAG2055.456968E-1215.3414635
GATAACG2300.015.28260911
GCTAGAC855.366958E-415.2352933
ACGAACG1954.1836756E-1115.17948715
ATCTCGC8050.015.16770211
GTAAACG2450.015.10204127
TCGCCAT8000.015.03125113
CTAGGAC2350.014.9574473
GTAGAAC11150.014.9327353
TCTCGCC8900.014.75842712
Produced by FastQC (version 0.11.5)