##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1630481.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2227761
Sequences flagged as poor quality	0
Sequence length	43
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.04470362844129	33.0	31.0	34.0	30.0	34.0
2	32.22569342043424	33.0	31.0	34.0	30.0	34.0
3	32.33472980270325	34.0	31.0	34.0	30.0	34.0
4	35.917990305064144	37.0	35.0	37.0	35.0	37.0
5	35.893560395392505	37.0	35.0	37.0	35.0	37.0
6	35.92297961944751	37.0	35.0	37.0	35.0	37.0
7	35.9048757923314	37.0	35.0	37.0	35.0	37.0
8	35.89530968537469	37.0	35.0	37.0	35.0	37.0
9	37.642659603072325	39.0	37.0	39.0	35.0	39.0
10	37.56086761551172	39.0	37.0	39.0	35.0	39.0
11	37.65043691850248	39.0	37.0	39.0	35.0	39.0
12	37.588542936158774	39.0	37.0	39.0	35.0	39.0
13	37.6317095954189	39.0	37.0	39.0	35.0	39.0
14	38.933119396560045	40.0	38.0	41.0	36.0	41.0
15	38.91595058895456	40.0	38.0	41.0	36.0	41.0
16	38.89597133624298	40.0	38.0	41.0	35.0	41.0
17	38.88787262188359	40.0	38.0	41.0	35.0	41.0
18	38.869510239204295	40.0	38.0	41.0	35.0	41.0
19	38.90844170447369	40.0	38.0	41.0	35.0	41.0
20	38.88584233227891	40.0	38.0	41.0	35.0	41.0
21	38.81287400219323	40.0	38.0	41.0	35.0	41.0
22	38.78141910195932	40.0	38.0	41.0	35.0	41.0
23	38.76104662932873	40.0	38.0	41.0	35.0	41.0
24	38.712270301886065	40.0	38.0	41.0	35.0	41.0
25	38.67114874530975	40.0	38.0	41.0	34.0	41.0
26	38.50190437843198	40.0	38.0	41.0	34.0	41.0
27	38.40180970938983	40.0	38.0	41.0	34.0	41.0
28	38.323394206110976	40.0	38.0	41.0	34.0	41.0
29	38.23643020952427	40.0	38.0	41.0	34.0	41.0
30	38.16870077176142	40.0	38.0	41.0	33.0	41.0
31	38.12764924065014	40.0	38.0	41.0	33.0	41.0
32	38.03693349511011	40.0	37.0	41.0	33.0	41.0
33	38.01608879947176	40.0	37.0	41.0	33.0	41.0
34	37.95146068182359	40.0	37.0	41.0	33.0	41.0
35	37.913587678390996	40.0	37.0	41.0	33.0	41.0
36	37.875886147571485	40.0	37.0	41.0	33.0	41.0
37	37.70957387260123	40.0	37.0	41.0	33.0	41.0
38	37.60455632359127	40.0	37.0	41.0	33.0	41.0
39	37.56256977296936	40.0	37.0	41.0	33.0	41.0
40	37.4840837953443	40.0	37.0	41.0	32.0	41.0
41	37.38352767644285	40.0	37.0	41.0	32.0	41.0
42	37.31662283341885	40.0	36.0	41.0	32.0	41.0
43	36.505515627574056	39.0	35.0	40.0	30.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	2.0
10	2.0
11	6.0
12	4.0
13	2.0
14	6.0
15	4.0
16	9.0
17	31.0
18	68.0
19	106.0
20	225.0
21	419.0
22	872.0
23	1425.0
24	2561.0
25	4132.0
26	6557.0
27	9916.0
28	14311.0
29	20907.0
30	28190.0
31	37635.0
32	50133.0
33	65350.0
34	87881.0
35	119936.0
36	170216.0
37	265382.0
38	486860.0
39	854613.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.34513441971558	19.506266605798377	12.311958060133021	24.83664091435302
2	18.017641928375618	21.083679981829288	36.200651685705964	24.698026404089127
3	19.591239814324787	23.58843700019885	30.8200475724281	26.000275613048256
4	14.209962379267793	15.497847390272115	34.78380310993863	35.508387120521455
5	13.722522299295123	37.803651289343875	34.79076076832299	13.68306564303801
6	34.4694516153214	35.31240559467555	15.81902187891789	14.399120911085166
7	28.145523689480157	31.400720274751198	21.730966652167805	18.722789383600844
8	26.23697066247232	34.77814720699393	20.291404688384436	18.693477442149316
9	25.80869312282601	14.039163087961409	20.25356400439724	39.89857978481533
10	16.08516353414931	27.7712465565202	33.773551112529574	22.370038796800916
11	34.90872674402685	21.847720648669224	22.646729159905394	20.59682344739853
12	20.71353255578134	26.220676275417336	29.537863352487093	23.52792781631423
13	28.93380394036883	20.16315933351917	26.478603404943353	24.424433321168653
14	22.114804954391428	21.726612504662754	25.753929618123312	30.40465292282251
15	25.153775472324007	28.73791219076014	23.10759547366167	23.00071686325418
16	24.882292130978144	27.361014040554622	24.38039807681345	23.376295751653792
17	23.292085641143732	27.473189448957946	25.89882846499243	23.33589644490589
18	22.3643380057376	25.86987562848977	27.824618529546036	23.941167836226597
19	23.662367731547505	26.415356045823586	27.592906061287543	22.329370161341362
20	23.95023523618557	25.380729800009966	27.778383767378994	22.89065119642547
21	23.811755390277504	26.697028990093642	26.327420221469	23.163795398159856
22	23.800757801218353	25.97311830128995	26.65357729128035	23.572546606211347
23	22.910087751783067	26.484753077192753	27.00128065802391	23.60387851300027
24	24.289320084156245	26.39250799345172	26.528429216599086	22.789742705792946
25	23.904628907679054	25.688617405547543	27.040423097450763	23.366330589322644
26	23.655634513756187	26.41531115770498	26.797802816370336	23.131251512168497
27	23.80268799031853	25.985058540839884	26.44709194567999	23.765161523161595
28	22.753742434668712	26.416657801263245	27.43557320556379	23.394026558504255
29	23.004397688980102	26.990058628371717	27.034318313319964	22.97122536932822
30	22.53971588514208	27.58424265439605	26.95181395131704	22.92422750914483
31	23.460371197808023	26.576145286680212	26.85624714679896	23.1072363687128
32	22.55578583160402	26.494179582100593	26.981484997717438	23.968549588577947
33	22.66665948456769	26.149124614354953	27.281382518142657	23.902833382934705
34	23.221656183046566	26.295908762205638	26.918910960376806	23.563524094370987
35	23.44106930680625	26.151234355929564	27.256873605382264	23.15082273188192
36	22.805139330475754	26.55235458381756	27.09190976949502	23.55059631621166
37	23.685036231444936	24.976153186989087	27.308898934849836	24.029911646716144
38	22.631601863934236	25.48684531240111	28.398872230908072	23.482680592756584
39	22.738480474341728	25.292928640011205	28.28279155618578	23.685799329461286
40	22.475346323057096	24.85607746971062	29.147067391879112	23.521508815353172
41	21.61807303386674	25.671694584832032	29.241511993431967	23.468720387869254
42	22.611267546204463	24.803468594701137	28.895065494009458	23.690198365084942
43	21.473174186997618	24.92278121396326	29.021784652842026	24.5822599461971
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	233.0
1	236.0
2	239.0
3	751.0
4	1263.0
5	1263.0
6	1701.0
7	2139.0
8	2114.0
9	2089.0
10	3223.0
11	4357.0
12	4357.0
13	6896.0
14	9435.0
15	14194.0
16	18953.0
17	19188.0
18	19423.0
19	19423.0
20	22807.5
21	26192.0
22	28523.0
23	30854.0
24	33839.5
25	36825.0
26	36825.0
27	39975.0
28	43125.0
29	54235.0
30	65345.0
31	68865.5
32	72386.0
33	72386.0
34	79549.5
35	86713.0
36	93162.5
37	99612.0
38	115709.5
39	131807.0
40	131807.0
41	140551.5
42	149296.0
43	141524.5
44	133753.0
45	140690.0
46	147627.0
47	147627.0
48	152944.5
49	158262.0
50	162364.5
51	166467.0
52	171769.5
53	177072.0
54	177072.0
55	155805.0
56	134538.0
57	125703.0
58	116868.0
59	107125.0
60	97382.0
61	97382.0
62	89684.5
63	81987.0
64	73336.0
65	64685.0
66	56775.0
67	48865.0
68	48865.0
69	42469.0
70	36073.0
71	31002.5
72	25932.0
73	20697.0
74	15462.0
75	15462.0
76	12195.0
77	8928.0
78	7250.5
79	5573.0
80	4410.0
81	3247.0
82	3247.0
83	2520.0
84	1793.0
85	1542.5
86	1292.0
87	1175.5
88	1059.0
89	1059.0
90	789.0
91	519.0
92	290.5
93	62.0
94	40.0
95	18.0
96	18.0
97	13.0
98	8.0
99	5.5
100	3.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	2227761.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	46.99316079797569
#Duplication Level	Percentage of deduplicated	Percentage of total
1	83.57660067661425	39.27528634544337
2	9.450662375812941	8.88232993347913
3	2.6571143533109955	3.7459860619125847
4	1.159323062327048	2.1792102033894625
5	0.7024255183508281	1.650459766623094
6	0.4289757622159227	1.2095356183348223
7	0.2907194525319263	0.9563278185952592
8	0.20780793877426887	0.7812441505532083
9	0.17013930092956797	0.7195845173974527
>10	1.0605292328523648	9.589375659000373
>50	0.12690160896788477	4.252587905663094
>100	0.14263408991217433	14.7767972082301
>500	0.019265538854808403	6.128714322446026
>1k	0.0067093916409780505	5.261561557348713
>5k	1.916969040279443E-4	0.5909989315833074
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	6713	0.3013339402206969	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	6408	0.287643064045021	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	4903	0.22008644553881676	No Hit
GCTTAGGACACAGAGCACATCAAAAGTTCCCAAAGAGGGCTTG	3612	0.1621358844148901	No Hit
GTTCTACAGCACACTACCAGAAGACAGCAGAAATGAAAAGCAT	3597	0.1614625626357585	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3238	0.1453477280552088	No Hit
CTGTAGAACAGAGCAGGTGAAGAGAGAGCAAGCCCTCTTTGGG	3101	0.13919805580580683	No Hit
TCCCAGAAGAGGTCGCCATTGTTGAAGAACTTGGCCGCAGACA	2978	0.13367681721692767	No Hit
CTTCTGGGAAATCTCGCCTTCCTCGGCCTTTCACCAGCCAAGC	2910	0.13062442515153105	No Hit
ATTGAAAGCTGAGTATTTTTAAGACAAAGGTTTCAGGAAGAAA	2365	0.10616040050974948	No Hit
GAATACACCTCTTAAATTTACAGGACTTAACATTTCAAACATC	2246	0.10081871439530542	No Hit
CTTCAAGACACAGAGGAGAAATCCAGATCATTCTCAGCTTCCC	2228	0.1000107282603475	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	4.488811860877356E-5	0.0
2	0.0	0.0	0.0	3.591049488701885E-4	0.0
3	0.0	0.0	0.0	5.835455419140564E-4	0.0
4	0.0	0.0	0.0	8.528742535666977E-4	0.0
5	0.0	0.0	0.0	9.875386093930183E-4	0.0
6	0.0	0.0	0.0	0.001032426728001792	0.0
7	0.0	0.0	0.0	0.0012568673210456597	0.0
8	0.0	0.0	0.0	0.0012568673210456597	0.0
9	0.0	0.0	0.0	0.0021097415746123573	0.0
10	0.0	4.488811860877356E-5	0.0	0.003187056421222923	0.0
11	0.0	4.488811860877356E-5	0.0	0.0034563851328755645	0.0
12	0.0	4.488811860877356E-5	0.0	0.004039930674789621	0.0
13	0.0	4.488811860877356E-5	0.0	0.004354147505051036	0.0
14	0.0	4.488811860877356E-5	0.0	0.004803028691138771	0.0
15	0.0	4.488811860877356E-5	0.0	0.005386574233052828	0.0
16	0.0	4.488811860877356E-5	0.0	0.006508777198272166	0.0
17	0.0	4.488811860877356E-5	0.0	0.008214525705405562	0.0
18	0.0	4.488811860877356E-5	0.0	0.00906739995897226	0.0
19	0.0	4.488811860877356E-5	0.0	0.01045893163584424	0.0
20	0.0	4.488811860877356E-5	0.0	0.011536246482454805	0.0
21	0.0	4.488811860877356E-5	0.0	0.013690876175675937	0.0
22	0.0	4.488811860877356E-5	0.0	0.01817968803655329	0.0
23	0.0	4.488811860877356E-5	0.0	0.026304437504741308	0.0
24	0.0	4.488811860877356E-5	0.0	0.03941176813850319	0.0
25	0.0	4.488811860877356E-5	0.0	0.04156639783172432	0.0
26	0.0	4.488811860877356E-5	0.0	0.04749162948808243	0.0
27	0.0	4.488811860877356E-5	0.0	0.05754656805644771	0.0
28	0.0	4.488811860877356E-5	0.0	0.0872625025754558	0.0
29	0.0	4.488811860877356E-5	0.0	0.13677409740093305	0.0
30	0.0	4.488811860877356E-5	0.0	0.20720355549809877	0.0
31	0.0	4.488811860877356E-5	0.0	0.48344503741649125	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGAATTA	230	0.0	20.108696	15
AGTACCG	60	9.2405255E-4	18.5	5
CTTATAC	1235	0.0	18.425102	37
GGTATCA	3145	0.0	17.529411	1
TACGTTA	195	1.8189894E-12	16.128206	19
CGAACGA	185	1.8189894E-11	16.0	16
CGGCCTT	810	0.0	15.987655	24
CCCGAAT	290	0.0	15.948276	13
TAGAACA	1085	0.0	15.857143	4
TCTTATA	2065	0.0	15.767555	37
TAGACAG	205	5.456968E-12	15.341463	5
GATAACG	230	0.0	15.282609	11
GCTAGAC	85	5.366958E-4	15.235293	3
ACGAACG	195	4.1836756E-11	15.179487	15
ATCTCGC	805	0.0	15.167702	11
GTAAACG	245	0.0	15.102041	27
TCGCCAT	800	0.0	15.031251	13
CTAGGAC	235	0.0	14.957447	3
GTAGAAC	1115	0.0	14.932735	3
TCTCGCC	890	0.0	14.758427	12
>>END_MODULE