FastQCFastQC Report
Fri 10 Feb 2017
ERR1630480.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630480.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1669879
Sequences flagged as poor quality0
Sequence length43
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT32970.19743945519405895No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT25410.152166713875676No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT21230.12713496007794575No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTAGAC400.001931551123.1250023
CTAGTAC450.003826522520.5555573
AAGACGG4250.020.4588245
GGTATCA20200.019.8737621
CGCAAGA4350.018.7126452
GCGCAAG4750.017.9157891
GACGGAC4350.017.4367837
AGACGGA4700.017.3191476
CAAGACG4600.017.2934764
CGGACCA4400.016.8181829
TCTTATA12750.016.10588337
CTTATAC7850.016.02547837
ACGGACC4800.015.4166668
TCTAGTA855.365958E-415.2352942
GCGTATA1353.974983E-715.0740759
TAGTACT1353.974983E-715.0740754
TAGAACA5350.014.8691594
TATACTG1252.9605035E-614.7999995
GCGAAAG5050.014.65346418
CGGTCCA3550.014.5915510
Produced by FastQC (version 0.11.5)