##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1630480.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1669879
Sequences flagged as poor quality	0
Sequence length	43
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.96936185196652	33.0	31.0	34.0	30.0	34.0
2	32.14691064442394	33.0	31.0	34.0	30.0	34.0
3	32.25278777683892	34.0	31.0	34.0	30.0	34.0
4	35.83923745373168	37.0	35.0	37.0	35.0	37.0
5	35.8136463779711	37.0	35.0	37.0	35.0	37.0
6	35.84285807534558	37.0	35.0	37.0	35.0	37.0
7	35.81965280119098	37.0	35.0	37.0	35.0	37.0
8	35.806542270427975	37.0	35.0	37.0	35.0	37.0
9	37.54628748550044	39.0	37.0	39.0	35.0	39.0
10	37.44690782984875	39.0	37.0	39.0	34.0	39.0
11	37.549290697110386	39.0	37.0	39.0	35.0	39.0
12	37.47420980801603	39.0	37.0	39.0	35.0	39.0
13	37.519676575368635	39.0	37.0	39.0	35.0	39.0
14	38.799860948008806	40.0	38.0	41.0	35.0	41.0
15	38.779092377351894	40.0	38.0	41.0	35.0	41.0
16	38.75420135231355	40.0	38.0	41.0	35.0	41.0
17	38.72946423064186	40.0	38.0	41.0	35.0	41.0
18	38.70931905844675	40.0	38.0	41.0	35.0	41.0
19	38.73843374280412	40.0	38.0	41.0	34.0	41.0
20	38.72031087282372	40.0	38.0	41.0	34.0	41.0
21	38.64437183771998	40.0	38.0	41.0	34.0	41.0
22	38.589258263622696	40.0	38.0	41.0	34.0	41.0
23	38.569367002040266	40.0	38.0	41.0	34.0	41.0
24	38.513979755419406	40.0	38.0	41.0	34.0	41.0
25	38.47739866181921	40.0	38.0	41.0	34.0	41.0
26	38.29371589198978	40.0	38.0	41.0	34.0	41.0
27	38.18459121888472	40.0	38.0	41.0	33.0	41.0
28	38.0903454681447	40.0	38.0	41.0	33.0	41.0
29	37.995186477583104	40.0	37.0	41.0	33.0	41.0
30	37.924319666275224	40.0	37.0	41.0	33.0	41.0
31	37.89658532145143	40.0	37.0	41.0	33.0	41.0
32	37.805208640865594	40.0	37.0	41.0	33.0	41.0
33	37.7729146842376	40.0	37.0	41.0	33.0	41.0
34	37.7045504494637	40.0	37.0	41.0	33.0	41.0
35	37.654903139688564	40.0	37.0	41.0	32.0	41.0
36	37.62392185302049	40.0	37.0	41.0	33.0	41.0
37	37.44659882542388	40.0	37.0	41.0	32.0	41.0
38	37.33868921041584	40.0	36.0	41.0	31.0	41.0
39	37.29147261568054	40.0	36.0	41.0	31.0	41.0
40	37.199402471676095	39.0	36.0	41.0	31.0	41.0
41	37.08835191052765	39.0	36.0	41.0	31.0	41.0
42	37.01251408036151	39.0	36.0	41.0	31.0	41.0
43	36.14130604672554	38.0	35.0	40.0	29.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	3.0
9	1.0
10	4.0
11	5.0
12	1.0
13	1.0
14	0.0
15	4.0
16	7.0
17	27.0
18	58.0
19	103.0
20	237.0
21	446.0
22	824.0
23	1515.0
24	2445.0
25	3975.0
26	5999.0
27	8964.0
28	12625.0
29	17677.0
30	24172.0
31	32017.0
32	41746.0
33	53593.0
34	70951.0
35	94605.0
36	134371.0
37	202326.0
38	373316.0
39	587861.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.75358933192165	18.545655104351873	11.88816674741104	25.812588816315436
2	19.6452557340981	20.606522987593713	33.72220382434895	26.026017453959238
3	20.949242430140146	22.46857407033683	28.83496349136674	27.747220008156283
4	15.141276703282095	15.08564392988953	33.11994461874184	36.653134748086536
5	15.098878421729959	36.092674978246926	33.8642500444643	14.944196555558817
6	36.3459867451474	33.34355363472443	15.129000364697081	15.181459255431081
7	29.785331751582	29.58423933710167	20.86923663331295	19.761192278003374
8	27.24927973823253	32.73332978018168	19.745981595073655	20.271408886512138
9	26.664746367850604	13.626077098999389	19.217080998084292	40.492095535065715
10	17.36431202500301	26.131713734947265	32.38336430364116	24.120609936408567
11	36.519891561005316	21.00655197172969	20.894627694581462	21.578928772683533
12	22.158910915102233	24.921745827092863	27.73961466669142	25.179728591113488
13	30.16260459590186	19.273971347624588	24.979354791574718	25.584069264898833
14	23.483497906135714	20.813484090763463	24.110908634697484	31.59210936840334
15	26.617138127972144	27.201372075461755	21.55096267454109	24.630527122025008
16	26.546953402012964	25.718570028127786	22.98753382730126	24.74694274255799
17	24.72065341261253	25.772226610431055	24.404822145796192	25.102297831160218
18	24.225707371611954	24.319486621485748	26.196508848844736	25.25829715805756
19	25.021753073126856	24.912942794058733	25.576883115483216	24.4884210173312
20	25.474181063418367	24.027489416897872	25.67964505212653	24.81868446755723
21	25.649223686267085	24.864436285503324	24.789281139531667	24.697058888697924
22	25.604430021576412	24.415960677390398	24.929231399400795	25.05037790163239
23	24.71580276175699	24.701190924611904	25.40273876131145	25.180267552319656
24	25.277819530636652	25.142959459936918	25.014806462025092	24.564414547401338
25	25.51250719363499	24.563456394145923	25.029178760856325	24.894857651362763
26	25.176554708454923	25.388845539107923	24.865394438758738	24.569205313678417
27	25.669883865836983	24.28463379682001	24.64454011338546	25.400942223957546
28	24.519501113553737	24.756224852219834	25.6770700152526	25.04720401897383
29	24.237744171883115	25.22092918109636	25.55017459348851	24.991152053532023
30	24.002697201413994	25.975594638892996	25.595507219385354	24.426200940307652
31	25.00318885380318	25.324050425210448	24.871981742389718	24.800778978596654
32	24.169236214120904	24.925279017222206	25.21140753312066	25.694077235536227
33	24.12150820508552	24.783711873734564	25.716893259930814	25.37788666124911
34	25.120562627591582	24.5875898792667	25.319199774354907	24.97264771878681
35	24.924081325652935	24.631485275280422	25.950742538830657	24.49369086023598
36	24.1018660633495	25.232247366425952	25.518375882324406	25.147510687900144
37	25.134036657745863	24.111088288432875	25.64802599469782	25.106849059123444
38	24.20834084385755	24.168517599179342	26.499045739242185	25.124095817720928
39	24.466623030770492	24.015093309155933	26.275975684465763	25.242307975607815
40	23.905684184303176	24.02281841977772	27.091783296873608	24.9797140990455
41	23.179523785855142	24.240259324178577	27.575171614230733	25.005045275735547
42	24.131089737639673	23.854842177187688	27.25640600306968	24.757662082102954
43	23.298514443262057	23.523919996598554	27.411926253339313	25.765639306800075
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	55.0
1	67.0
2	79.0
3	234.5
4	390.0
5	390.0
6	635.5
7	881.0
8	906.0
9	931.0
10	1306.5
11	1682.0
12	1682.0
13	2959.5
14	4237.0
15	5968.0
16	7699.0
17	7782.5
18	7866.0
19	7866.0
20	9825.5
21	11785.0
22	12369.0
23	12953.0
24	14973.5
25	16994.0
26	16994.0
27	19651.5
28	22309.0
29	28167.5
30	34026.0
31	38257.5
32	42489.0
33	42489.0
34	47886.5
35	53284.0
36	59204.0
37	65124.0
38	75196.5
39	85269.0
40	85269.0
41	90146.0
42	95023.0
43	94693.5
44	94364.0
45	99339.5
46	104315.0
47	104315.0
48	109284.0
49	114253.0
50	118757.0
51	123261.0
52	130431.0
53	137601.0
54	137601.0
55	129042.0
56	120483.0
57	115055.5
58	109628.0
59	102280.0
60	94932.0
61	94932.0
62	89652.5
63	84373.0
64	75671.0
65	66969.0
66	58975.0
67	50981.0
68	50981.0
69	44363.0
70	37745.0
71	32870.0
72	27995.0
73	22047.5
74	16100.0
75	16100.0
76	12626.0
77	9152.0
78	7599.0
79	6046.0
80	4838.5
81	3631.0
82	3631.0
83	2786.0
84	1941.0
85	1656.0
86	1371.0
87	1168.5
88	966.0
89	966.0
90	785.0
91	604.0
92	335.0
93	66.0
94	40.5
95	15.0
96	15.0
97	12.0
98	9.0
99	5.5
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1669879.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	54.02106882857674
#Duplication Level	Percentage of deduplicated	Percentage of total
1	85.50220481951591	46.18920491550135
2	8.720996102407472	9.422350614038068
3	2.282980844466788	3.6998719599978767
4	0.9943837845213752	2.148706994625993
5	0.5436509480038498	1.4684302640418483
6	0.3597199770094362	1.1659474582224476
7	0.23389267850133033	0.8844592738674375
8	0.17675154171941831	0.7638645760625399
9	0.11266749422864453	0.547777661442199
>10	0.7943745109372933	8.528647220299144
>50	0.12757298903655992	4.903593832909842
>100	0.1382651602813046	14.942162078464213
>500	0.010541762745294437	3.7432641501456523
>1k	0.001997386625424209	1.591719000381416
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	3297	0.19743945519405895	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	2541	0.152166713875676	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	2123	0.12713496007794575	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0012575761477328596	0.0
3	0.0	0.0	0.0	0.0017366527754406158	0.0
4	0.0	0.0	0.0	0.003173882658563884	0.0
5	0.0	0.0	0.0	0.003533190129344701	0.0
6	0.0	0.0	0.0	0.00365295928627164	0.0
7	0.0	0.0	0.0	0.004072151335515926	0.0
8	0.0	0.0	0.0	0.004551227963223683	0.0
9	0.0	0.0	0.0	0.007425687729470219	0.0
10	0.0	0.0	0.0	0.01107864701574186	0.0
11	0.0	0.0	0.0	0.012515876898865127	0.0
12	5.9884578463469506E-5	0.0	0.0	0.013294376418890232	0.0
13	5.9884578463469506E-5	0.0	0.0	0.013893222203524926	0.0
14	5.9884578463469506E-5	0.0	0.0	0.014432183409696151	0.0
15	5.9884578463469506E-5	0.0	0.0	0.015330452086648193	0.0
16	5.9884578463469506E-5	0.0	0.0	0.01706710486208881	0.0
17	5.9884578463469506E-5	0.0	0.0	0.01844445016674861	0.0
18	5.9884578463469506E-5	0.0	0.0	0.019761910892944937	0.0
19	5.9884578463469506E-5	0.0	0.0	0.021678217403775964	0.0
20	5.9884578463469506E-5	0.0	0.0	0.023534639336143515	0.0
21	5.9884578463469506E-5	0.0	0.0	0.025930022474682297	0.0
22	5.9884578463469506E-5	0.0	0.0	0.028984135976319242	0.0
23	5.9884578463469506E-5	5.9884578463469506E-5	0.0	0.03287663357644476	0.0
24	5.9884578463469506E-5	5.9884578463469506E-5	0.0	0.04018255214898804	0.0
25	1.1976915692693901E-4	5.9884578463469506E-5	0.0	0.04257793528752682	0.0
26	1.1976915692693901E-4	5.9884578463469506E-5	0.0	0.049105354340044996	0.0
27	1.1976915692693901E-4	5.9884578463469506E-5	0.0	0.06078284714042155	0.0
28	1.1976915692693901E-4	5.9884578463469506E-5	0.0	0.08557506262429793	0.0
29	1.1976915692693901E-4	5.9884578463469506E-5	0.0	0.1250988844101878	0.0
30	1.1976915692693901E-4	5.9884578463469506E-5	0.0	0.17935431249809117	0.0
31	1.1976915692693901E-4	5.9884578463469506E-5	0.0	0.3635592758517234	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTTAGAC	40	0.0019315511	23.125002	3
CTAGTAC	45	0.0038265225	20.555557	3
AAGACGG	425	0.0	20.458824	5
GGTATCA	2020	0.0	19.873762	1
CGCAAGA	435	0.0	18.712645	2
GCGCAAG	475	0.0	17.915789	1
GACGGAC	435	0.0	17.436783	7
AGACGGA	470	0.0	17.319147	6
CAAGACG	460	0.0	17.293476	4
CGGACCA	440	0.0	16.818182	9
TCTTATA	1275	0.0	16.105883	37
CTTATAC	785	0.0	16.025478	37
ACGGACC	480	0.0	15.416666	8
TCTAGTA	85	5.365958E-4	15.235294	2
GCGTATA	135	3.974983E-7	15.074075	9
TAGTACT	135	3.974983E-7	15.074075	4
TAGAACA	535	0.0	14.869159	4
TATACTG	125	2.9605035E-6	14.799999	5
GCGAAAG	505	0.0	14.653464	18
CGGTCCA	355	0.0	14.59155	10
>>END_MODULE