FastQCFastQC Report
Fri 10 Feb 2017
ERR1630479.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630479.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1577763
Sequences flagged as poor quality0
Sequence length43
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT72000.45634230236100104No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT66380.42072225042671174No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT55260.3502427170620683No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT42610.27006590977225353No Hit
ACCTAGGCCTGGTCAGCATGTTGATGTATCTACGGAGATCAGC20380.12917022391829444No Hit
GGCCTAGGTATGGGAAAAGACACAAAGAGGACACGCTGGCCTT16350.10362773116114397No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAAGACG4250.019.152944
TAATTCG609.238894E-418.55
TCGCTTA500.007036015418.523
CGCAAGA3850.017.779222
AAGACGG3750.017.7600025
TCTACAC1800.017.4722213
GACGGAC3800.017.0394747
TCTAATA2450.016.6122442
CGGACCA3900.016.1282049
TAATACT3000.016.0333334
CGAAAGC4200.015.41666619
CTAACAC2055.456968E-1215.3414633
GGTATCA36250.015.2593121
ACGGACC4250.015.2352948
TATACTG1701.4861143E-915.2352945
TCTAGCG2800.015.19642828
CTAGCGG2800.015.19642829
GCGCAAG4200.014.976191
ACGAACG2350.014.95744715
GCGAAAG4300.014.62790818
Produced by FastQC (version 0.11.5)