##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1630479.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1577763
Sequences flagged as poor quality	0
Sequence length	43
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.968249984313232	33.0	31.0	34.0	30.0	34.0
2	32.134930277868094	33.0	31.0	34.0	30.0	34.0
3	32.23851744526903	34.0	31.0	34.0	30.0	34.0
4	35.82326559819187	37.0	35.0	37.0	35.0	37.0
5	35.80236385312623	37.0	35.0	37.0	35.0	37.0
6	35.83805489164089	37.0	35.0	37.0	35.0	37.0
7	35.80951068062821	37.0	35.0	37.0	35.0	37.0
8	35.80863475693118	37.0	35.0	37.0	35.0	37.0
9	37.521731083819304	39.0	37.0	39.0	35.0	39.0
10	37.42513355934953	39.0	37.0	39.0	34.0	39.0
11	37.53424944050532	39.0	37.0	39.0	35.0	39.0
12	37.466471833855906	39.0	37.0	39.0	35.0	39.0
13	37.51970416342632	39.0	37.0	39.0	35.0	39.0
14	38.78204014164358	40.0	38.0	41.0	35.0	41.0
15	38.7487994077691	40.0	38.0	41.0	35.0	41.0
16	38.73036508017998	40.0	38.0	41.0	35.0	41.0
17	38.72978894802325	40.0	38.0	41.0	35.0	41.0
18	38.723016067685705	40.0	38.0	41.0	35.0	41.0
19	38.74174765158012	40.0	38.0	41.0	34.0	41.0
20	38.72820949661008	40.0	38.0	41.0	34.0	41.0
21	38.65219808044681	40.0	38.0	41.0	34.0	41.0
22	38.594660288015376	40.0	38.0	41.0	34.0	41.0
23	38.56534156270619	40.0	38.0	41.0	34.0	41.0
24	38.510892954138235	40.0	38.0	41.0	34.0	41.0
25	38.46807790523672	40.0	38.0	41.0	34.0	41.0
26	38.27139564053663	40.0	38.0	41.0	34.0	41.0
27	38.14962576762163	40.0	38.0	41.0	33.0	41.0
28	38.04230166381136	40.0	37.0	41.0	33.0	41.0
29	37.937762515662996	40.0	37.0	41.0	33.0	41.0
30	37.85121719802024	40.0	37.0	41.0	33.0	41.0
31	37.794349975249766	40.0	37.0	41.0	33.0	41.0
32	37.697945128640995	40.0	37.0	41.0	33.0	41.0
33	37.64305412156325	40.0	37.0	41.0	33.0	41.0
34	37.560564546132724	40.0	37.0	41.0	32.0	41.0
35	37.48944486592727	40.0	36.0	41.0	32.0	41.0
36	37.43920221224607	40.0	36.0	41.0	32.0	41.0
37	37.239778090879305	40.0	36.0	41.0	31.0	41.0
38	37.11909900282869	40.0	36.0	41.0	31.0	41.0
39	37.03901790066062	39.0	36.0	41.0	31.0	41.0
40	36.90613799410938	39.0	35.0	41.0	31.0	41.0
41	36.76797529159956	39.0	35.0	41.0	30.0	41.0
42	36.654766907323854	39.0	35.0	41.0	30.0	41.0
43	35.80772714279648	38.0	35.0	40.0	28.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	4.0
10	6.0
11	5.0
12	5.0
13	3.0
14	5.0
15	3.0
16	6.0
17	28.0
18	70.0
19	123.0
20	240.0
21	459.0
22	838.0
23	1481.0
24	2485.0
25	4120.0
26	6051.0
27	8993.0
28	12950.0
29	18083.0
30	24150.0
31	31527.0
32	40278.0
33	52184.0
34	68539.0
35	92112.0
36	128405.0
37	197522.0
38	351483.0
39	535604.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.20765349421934	19.349484047984394	12.173437962482325	24.269424495313935
2	19.222278631201263	21.068056482500857	33.647448951458486	26.06221593483939
3	21.169782787402163	23.63789745354657	28.53299259774757	26.659327161303693
4	14.474544022137673	15.190938055969117	34.072607863158154	36.26191005873506
5	14.533868521444601	37.23822906228629	33.65137856572882	14.57652385054029
6	35.468064595252905	33.88094409616653	15.429567051578722	15.221424257001845
7	29.174216913440105	30.28331885080332	21.50658875889471	19.03587547686186
8	28.336258360729715	31.925200426173006	19.8700945579279	19.868446655169375
9	26.832990759702184	13.61478244831448	20.34019051023506	39.212036281748276
10	17.993640362969597	26.50271301836841	32.071039820302545	23.432606798359448
11	35.55920629397444	22.029544361225355	21.05728173369511	21.35396761110509
12	22.627289396442936	25.84925619373759	28.204616282673634	23.31883812714584
13	30.2371775735646	19.676529364676444	25.582866374734355	24.503426687024604
14	23.110758713444287	21.10202863167662	25.419470478138983	30.367742176740105
15	26.068300498870872	27.681914203844304	22.634007769227697	23.61577752805713
16	25.241053314090898	26.011954900704353	24.638047666221098	24.10894411898365
17	23.8992801833989	26.269598158912334	25.490647201132237	24.340474456556528
18	23.39692336554983	24.5674413711058	27.665561938009702	24.37007332533467
19	24.326403902233732	25.089699783807834	27.438151357333133	23.145744956625297
20	24.795358998784987	24.376981840745408	26.939597392003744	23.88806176846586
21	25.20074307738234	24.799351993930646	26.72289817925759	23.277006749429415
22	25.008128597260804	24.770196791279805	26.769419741748283	23.452254869711105
23	24.091387616517817	24.85335249971003	26.694313404484703	24.360946479287445
24	23.711672792428267	25.73827628103841	26.84313169975465	23.706919226778673
25	24.182339172613375	25.29467353461832	26.53408655165573	23.988900741112577
26	24.00208396318078	25.975827801767437	26.696721877747166	23.325366357304613
27	24.440362716073327	24.80930279135713	26.613059122314315	24.137275370255228
28	23.525523161590176	25.443618591638923	27.166184021301042	23.86467422546986
29	23.544474043313222	25.727121246980694	27.359749214552505	23.368655495153583
30	23.513163890901232	25.969806618611287	26.888449025614115	23.62858046487337
31	23.347042616666762	26.309971776496216	26.70039796851618	23.64258763832084
32	23.067025909468025	25.633064027994063	26.590432149822245	24.70947791271566
33	22.606690611961366	25.989454689962944	27.156359985625215	24.247494712450475
34	24.313157299290197	24.946712529068055	26.88984340487133	23.85028676677042
35	23.547452944453635	25.24682097374574	27.24205092906856	23.963675152732065
36	22.70784648898472	26.116913630247378	27.068323949794742	24.106915930973155
37	23.491360869788426	24.899430396073427	27.610103672097775	23.99910506204037
38	23.18523124195459	24.909888240502536	27.505208323430068	24.399672194112803
39	23.207921595321984	24.734640120220845	27.70213270307391	24.355305581383263
40	22.551105584298785	24.763478418495048	28.224137592274634	24.461278404931537
41	21.61718838634193	25.127664928129256	28.826953097518448	24.428193588010366
42	22.41737193735688	24.974283209835697	28.92367231326885	23.684672539538575
43	22.303983551395234	24.52427899500749	28.55283081172521	24.61890664187207
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	543.0
1	492.5
2	442.0
3	1006.5
4	1571.0
5	1571.0
6	2066.0
7	2561.0
8	2415.5
9	2270.0
10	3429.5
11	4589.0
12	4589.0
13	7796.0
14	11003.0
15	14473.5
16	17944.0
17	17094.5
18	16245.0
19	16245.0
20	18522.5
21	20800.0
22	18730.5
23	16661.0
24	18744.5
25	20828.0
26	20828.0
27	23796.5
28	26765.0
29	30835.0
30	34905.0
31	39535.0
32	44165.0
33	44165.0
34	49813.0
35	55461.0
36	61398.0
37	67335.0
38	73508.5
39	79682.0
40	79682.0
41	82766.5
42	85851.0
43	88190.0
44	90529.0
45	93551.0
46	96573.0
47	96573.0
48	101703.0
49	106833.0
50	106067.5
51	105302.0
52	105691.5
53	106081.0
54	106081.0
55	102294.5
56	98508.0
57	95936.5
58	93365.0
59	92836.5
60	92308.0
61	92308.0
62	85388.0
63	78468.0
64	71433.0
65	64398.0
66	55895.0
67	47392.0
68	47392.0
69	41790.5
70	36189.0
71	30536.5
72	24884.0
73	18501.5
74	12119.0
75	12119.0
76	8882.5
77	5646.0
78	4508.0
79	3370.0
80	2872.0
81	2374.0
82	2374.0
83	1840.5
84	1307.0
85	1188.5
86	1070.0
87	987.0
88	904.0
89	904.0
90	675.5
91	447.0
92	248.5
93	50.0
94	32.5
95	15.0
96	15.0
97	10.5
98	6.0
99	5.0
100	4.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1577763.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	56.44454972570651
#Duplication Level	Percentage of deduplicated	Percentage of total
1	87.11455076911415	49.171415927198474
2	7.804932672426548	8.810918206691433
3	1.9602764624956561	3.319407667904043
4	0.8318642590738689	1.8781681414533193
5	0.44726064488681133	1.262271285533259
6	0.31312908677693185	1.0604658185487361
7	0.20060570075471443	0.7926168916056775
8	0.15682711167996688	0.7081628562847055
9	0.1168534629626019	0.5936166990732228
>10	0.8235785576358764	9.219478252149383
>50	0.1107632723932276	4.368542056500383
>100	0.1073096946173406	12.282980524471624
>500	0.009796285522058171	3.6803384661025023
>1k	0.001914216711206769	1.6208971764758766
>5k	3.3780294903648867E-4	1.230720030007406
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	7200	0.45634230236100104	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	6638	0.42072225042671174	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	5526	0.3502427170620683	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4261	0.27006590977225353	No Hit
ACCTAGGCCTGGTCAGCATGTTGATGTATCTACGGAGATCAGC	2038	0.12917022391829444	No Hit
GGCCTAGGTATGGGAAAAGACACAAAGAGGACACGCTGGCCTT	1635	0.10362773116114397	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	6.33808753279168E-5	0.0
2	0.0	0.0	0.0	4.4366612729541765E-4	0.0
3	6.33808753279168E-5	0.0	0.0	8.873322545908353E-4	0.0
4	6.33808753279168E-5	0.0	0.0	0.0012042366312304193	0.0
5	6.33808753279168E-5	0.0	0.0	0.0014577601325420865	0.0
6	6.33808753279168E-5	0.0	0.0	0.0015211410078700032	0.0
7	6.33808753279168E-5	0.0	0.0	0.0017112836338537536	0.0
8	6.33808753279168E-5	0.0	0.0	0.001901426259837504	0.0
9	6.33808753279168E-5	0.0	0.0	0.0031056628910679234	0.0
10	6.33808753279168E-5	0.0	0.0	0.005450755278200845	0.0
11	6.33808753279168E-5	0.0	0.0	0.006401468408119598	0.0
12	6.33808753279168E-5	0.0	0.0	0.007669085914677933	0.0
13	6.33808753279168E-5	0.0	0.0	0.008176132917301267	0.0
14	6.33808753279168E-5	0.0	0.0	0.00931698867320377	0.0
15	6.33808753279168E-5	0.0	0.0	0.01191560456164836	0.0
16	6.33808753279168E-5	0.0	0.0	0.014894505702060449	0.0
17	6.33808753279168E-5	0.0	0.0	0.020281880104933377	0.0
18	6.33808753279168E-5	0.0	0.0	0.022500210741410464	0.0
19	6.33808753279168E-5	0.0	0.0	0.026049539759773806	0.0
20	6.33808753279168E-5	0.0	0.0	0.02877491739887423	0.0
21	6.33808753279168E-5	0.0	0.0	0.03308481692117257	0.0
22	6.33808753279168E-5	0.0	0.0	0.044429993604869676	0.0
23	6.33808753279168E-5	0.0	0.0	0.06204987694603055	0.0
24	6.33808753279168E-5	0.0	0.0	0.08911351071105103	0.0
25	6.33808753279168E-5	0.0	0.0	0.09538821736851479	0.0
26	6.33808753279168E-5	0.0	0.0	0.10888834381336107	0.0
27	6.33808753279168E-5	0.0	0.0	0.12872655779099904	0.0
28	6.33808753279168E-5	0.0	0.0	0.17531150115701788	0.0
29	6.33808753279168E-5	0.0	0.0	0.23957970873952553	0.0
30	6.33808753279168E-5	0.0	0.0	0.33211578671828407	0.0
31	6.33808753279168E-5	0.0	0.0	0.6209424355876009	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CAAGACG	425	0.0	19.15294	4
TAATTCG	60	9.238894E-4	18.5	5
TCGCTTA	50	0.0070360154	18.5	23
CGCAAGA	385	0.0	17.77922	2
AAGACGG	375	0.0	17.760002	5
TCTACAC	180	0.0	17.472221	3
GACGGAC	380	0.0	17.039474	7
TCTAATA	245	0.0	16.612244	2
CGGACCA	390	0.0	16.128204	9
TAATACT	300	0.0	16.033333	4
CGAAAGC	420	0.0	15.416666	19
CTAACAC	205	5.456968E-12	15.341463	3
GGTATCA	3625	0.0	15.259312	1
ACGGACC	425	0.0	15.235294	8
TATACTG	170	1.4861143E-9	15.235294	5
TCTAGCG	280	0.0	15.196428	28
CTAGCGG	280	0.0	15.196428	29
GCGCAAG	420	0.0	14.97619	1
ACGAACG	235	0.0	14.957447	15
GCGAAAG	430	0.0	14.627908	18
>>END_MODULE