FastQCFastQC Report
Fri 10 Feb 2017
ERR1630478.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630478.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences497490
Sequences flagged as poor quality0
Sequence length43
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT21390.4299583911234397No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA18310.36804759894671246No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT16730.3362881665963135No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC16140.32442863173129105No Hit
CTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCGAGATTGAGC15480.3111620334077067No Hit
CTTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGT15280.3071418520975296No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGAT14810.29769442601861346No Hit
TCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTT14360.288649018070715TruSeq Adapter, Index 11 (95% over 21bp)
TGCCTACCCTACGCCGGCAGGCGCGGGTAACCCGTTGAACCCC12800.2572916038513337No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT12770.2566885766548071No Hit
CTACCATGGTGACCACGGGTGACGGGGAATCAGGGTTCGATTC12380.2488492230999618No Hit
GGATTACTCCGGTCTGAACTCAGATCACGTAGGACTTTAATCG12340.24804518683792637No Hit
TCTTAGAGGGACAAGTGGCGTTCAGCCACCCGAGATTGAGCAA12100.2432209692657139No Hit
CCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAATC12070.24261794206918733No Hit
GGGTTCAACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGGCAC11750.23618565197290395No Hit
CTCCGGAATCGAACCCTGATTCCCCGTCACCCGTGGTCACCAT11390.2289493256145852No Hit
GGTCTGTGATGCCCTTAGATGTCCGGGGCTGCACGCGCGCTAC10560.21226557317735031No Hit
ATTCCGGAGAGGGAGCCTGAGAAACGGCTACCACATCCAAGGA10330.20764236467064665No Hit
CCATGGTAGGCACGGCGACTACCATCGAAAGTTGATAGGGCAG10070.20241612896741645No Hit
ATTCGAACGTCTGCCCTATCAACTTTCGATGGTAGTCGCCGTG10030.20161209270538102No Hit
CGTCTGCCCTATCAACTTTCGATGGTAGTCGCCGTGCCTACCA10010.20121007457436332No Hit
CCTTAGATGTCCGGGGCTGCACGCGCGCTACACTGACTGGCTC9870.19839594765723936No Hit
AGTCAGTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATC9820.19739090232969506No Hit
GCGCTACACTGACTGGCTCAGCGTGTGCCTACCCTACGCCGGC9800.19698888419867736No Hit
CTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCG8850.17789302297533618No Hit
ACACTGACTGGCTCAGCGTGTGCCTACCCTACGCCGGCAGGCG8820.17728999577880963No Hit
GAGTAATCCAGGTCGGTTTCTATCTACTTCAAATTCCTCCCTG8730.17548091418922993No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8660.17407385073066795No Hit
CTCTAATACTGGTGATGCTAGAGGTGATGTTTTTGGTAAACAG8210.1650284427827695No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATG8080.1624153249311544No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCC7950.1598022070795393No Hit
ACCCGAGATTGAGCAATAACAGGTCTGTGATGCCCTTAGATGT7770.1561840439003799No Hit
CATCCAAGGAAGGCAGCAGGCGCGCAAATTACCCACTCCCGAC7460.14995276286960543No Hit
GGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGG7440.1495507447385877No Hit
CTACTATACTCAATTGATCCAATAACTTGACCAACGGAACAAG7440.1495507447385877No Hit
CATCTAAGGGCATCACAGACCTGTTATTGCTCAATCTCGGGTG7410.14894771754206115No Hit
GCCCCGGACATCTAAGGGCATCACAGACCTGTTATTGCTCAAT7270.1461335906249372No Hit
TCACAGACCTGTTATTGCTCAATCTCGGGTGGCTGAACGCCAC7200.1447265271663752No Hit
ATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATG7130.14331946370781323No Hit
ACGTAGGACTTTAATCGTTGAACAAACGAACCTTTAATAGCGG7080.14231441838026895No Hit
GATCAGGACATCCCGATGGTGCAGCCGCTATTAAAGGTTCGTT7060.14191240024925125No Hit
CATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTC6990.14050533679068924No Hit
GTCCGGGGCTGCACGCGCGCTACACTGACTGGCTCAGCGTGTG6970.14010331865967157No Hit
ACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCC6950.13970130052865384No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTC6700.13467607389093247No Hit
GATAGAAACCGACCTGGATTACTCCGGTCTGAACTCAGATCAC6580.13226396510482621No Hit
GTCCTGATCCAACATCGAGGTCGTAAACCCTATTGTTGATATG6520.1310579107117731No Hit
GTCCTAAACTACTAAACCTGCATTAAAAATTTCGGTTGGGGCG6440.12944983818770225No Hit
AGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCC6420.12904782005668455No Hit
GGGCAGACGTTCGAATGGGTCGTCGCCGCCACGGGGGGCGTGC6390.128444792860158No Hit
GTTATCCCTAGGGTAACTTGTTCCGTTGGTCAAGTTATTGGAT6240.12542965687752516No Hit
CGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGACATCT6220.12502763874650746No Hit
TGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTCAATTG6130.12321855715692778No Hit
AGGGATAACAGCGCAATCCTATTCTAGAGTCCATATCAACAAT6120.12301754809141892No Hit
AGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGA6090.12241452089489235No Hit
ATGGTAGGCACGGCGACTACCATCGAAAGTTGATAGGGCAGAC6060.12181149369836579No Hit
ACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATC5930.11919837584675069No Hit
ATTCTAGAGTCCATATCAACAATAGGGTTTACGACCTCGATGT5890.11839433958471526No Hit
GCCTACCATGGTGACCACGGGTGACGGGGAATCAGGGTTCGAT5870.11799232145369756No Hit
AGATTGAGCAATAACAGGTCTGTGATGCCCTTAGATGTCCGGG5820.1169872761261533No Hit
GTGCCTACCATGGTGACCACGGGTGACGGGGAATCAGGGTTCG5750.1155802126675913No Hit
GAATCAGGGTTCGATTCCGGAGAGGGAGCCTGAGAAACGGCTA5690.11437415827453819No Hit
CTATTAAAGGTTCGTTTGTTCAACGATTAAAGTCCTACGTGAT5660.11377113107801162No Hit
ATCCTATTCTAGAGTCCATATCAACAATAGGGTTTACGACCTC5570.11196204948843193No Hit
GACAAGTGGCGTTCAGCCACCCGAGATTGAGCAATAACAGGTC5530.1111580132263965No Hit
ACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCGAGATTGAG5410.10874590444029027No Hit
GGTCTGAACTCAGATCACGTAGGACTTTAATCGTTGAACAAAC5400.10854489537478139No Hit
GCCCTTAGATGTCCGGGGCTGCACGCGCGCTACACTGACTGGC5360.10774085911274599No Hit
TAACAGGTCTGTGATGCCCTTAGATGTCCGGGGCTGCACGCGC5320.10693682285071056No Hit
GTTCAACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGGCACAC5300.10653480471969287No Hit
ATCGAACCCTGATTCCCCGTCACCCGTGGTCACCATGGTAGGC5290.10633379565418401No Hit
GTACATGGGCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCAC5280.10613278658867516No Hit
GTACGGGGGAAGGCGCTTTGTGAAGTAGGCCTTATTTCTCTTG5280.10613278658867516No Hit
GTCTTAGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGT5250.10552975939214859No Hit
GGGTTCGATTCCGGAGAGGGAGCCTGAGAAACGGCTACCACAT5250.10552975939214859No Hit
ACTATACTCAATTGATCCAATAACTTGACCAACGGAACAAGTT5220.10492673219562201No Hit
GAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGACATCTAAGG5210.10472572313011316No Hit
CCCCGATCCCCATCACGAATGGGGTTCAACGGGTTACCCGCGC5190.10432370499909545No Hit
GTTTACGACCTCGATGTTGGATCAGGACATCCCGATGGTGCAG5160.1037206778025689No Hit
CCATCGGGATGTCCTGATCCAACATCGAGGTCGTAAACCCTAT5070.10191159621298922No Hit
GTGATGCCCTTAGATGTCCGGGGCTGCACGCGCGCTACACTGA5070.10191159621298922No Hit
TATCTAGAGTCACCAAAGCCGCCGGCGCCCGCCCCCCGGCCGG5020.10090655088544494No Hit
CTACTAAACCTGCATTAAAAATTTCGGTTGGGGCGACCTCGGA5010.10070554181993607No Hit
GGTGACGGGGAATCAGGGTTCGATTCCGGAGAGGGAGCCTGAG5010.10070554181993607No Hit
TCTAAGGGCATCACAGACCTGTTATTGCTCAATCTCGGGTGGC4990.10030352368891836No Hit
TGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAATCGAACCCTGA4990.10030352368891836No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATA200.001840932937.013
CTACGTA200.001840932937.09
CGTACAT200.001840932937.012
GCGCTCT200.001840932937.015
CGTCACT551.2732926E-1137.01
TACGTAC200.001840932937.010
TCATGGA251.2326193E-436.9999962
CTAGACT250.005493384329.5999984
CGCTCTC250.005493384329.59999816
ACGTACA250.005493384329.59999811
CCCGACT250.005493384329.59999828
TCGCAGA358.8622974E-426.4285721
CGTCTTC706.5738277E-926.4285737
GGGCGTC358.8622974E-426.4285713
ACACTTA358.8622974E-426.428576
TGACGAA659.3694325E-825.61538521
ACTTATC451.3217388E-424.6666668
GCCGTCT2550.024.66666636
TATACAC2500.024.4200023
CCGTCTT2550.023.94117537
Produced by FastQC (version 0.11.5)