FastQCFastQC Report
Fri 10 Feb 2017
ERR1630477.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630477.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2366969
Sequences flagged as poor quality0
Sequence length43
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCAGGGCCAGGACGATGGACAGCGCAGCCAGCGCGCACTGG61550.26003720369806277No Hit
GCCTGGGACAGATCTTCAGGTTCCAGGGCATCATTCTCCGTCT51390.21711310963514943No Hit
GTCCCAGGCTGCTGAGCAGGATGAAATGAGGCTTGAGCTGCAG46840.19789021317980926No Hit
GTCTGAACCCAACCAGACGGAGAATGATGCCCTGGAACCTGAA44430.18770841527709067No Hit
GCCCTGGGCTGTGTCACCGGCGCTCCCTCGGACCCCAGACTCC43930.1855960090732071No Hit
GATATGGACAATACTAGTTAAGAAAGCTAACAGGATGTGAAAG41290.17444250431670208No Hit
AGCAGGAACTGGCCAAGTACTTCTTGGCAGAGCTGCTGTCTGA37310.15762775093378917No Hit
GGTTCAGACAGCAGCTCTGCCAAGAAGTACTTGGCCAGTTCCT37140.15690953282446876No Hit
ACGATGGACAGCGCAGCCAGCGCGCACTGGAGGCGGCAGGACA35710.15086805108136184No Hit
GGCCAGGACGATGGACAGCGCAGCCAGCGCGCACTGGAGGCGG35400.14955835923495406No Hit
CTGCTGATCCGCGCCTAGAGTTTGACCAGCCACTCTCCAGCTC35220.148797893001556No Hit
CTTTAGGAGCGAGGTTCGGAGCCATCGCTGCTGCCTGCTGATC35120.14837541176077929No Hit
GCCAAGAAGTACTTGGCCAGTTCCTGCTTCCCCGCGGCAGCAG34990.14782618614776957No Hit
CTTCTTGGCAGAGCTGCTGTCTGAACCCAACCAGACGGAGAAT33790.1427564112584491No Hit
GTCCATATCAGACCTCTGATCCCTCGCCCCCACACCCCATCTC31870.13464477143553633No Hit
GGATGAAATGAGGCTTGAGCTGCAGAGATCTGCTAACTCAAAC31720.1340110495743713No Hit
ATTTCATCCTGCTCAGCAGCCTGGGACAGATCTTCAGGTTCCA30760.1299552296629149No Hit
GTCCATCGTCCTGGCCCTGGGCTGTGTCACCGGCGCTCCCTCG30600.12927925967767218No Hit
GTCCTGGCCCTGGGCTGTGTCACCGGCGCTCCCTCGGACCCCA30560.12911026718136148No Hit
GTTCCTGCTTCCCCGCGGCAGCAGCCAGGGACTTCTGCAGAAA30270.12788507158310902No Hit
AGCTAACAGGATGTGAAAGTCTTCCAGAAGAAATTCTTGCAGC30260.12784282345903136No Hit
CCCTCGGACCCCAGACTCCGTCAGTTTCTGCAGAAGTCCCTGG29570.12492770289767208No Hit
GATCAGCAGGCAGCAGCGATGGCTCCGAACCTCGCTCCTAAAG28490.12036490549728367No Hit
GAGTTTGACCAGCCACTCTCCAGCTCGGCTTTCGCGGCGCCGA26160.11052109258718638No Hit
CGGCAGGACAGCATCTCGGCGCCGCGAAAGCCGAGCTGGAGAG24830.10490209208485621No Hit
CTCCAGCTCGGCTTTCGCGGCGCCGAGATGCTGTCCTGCCGCC24600.10393038523106979No Hit
ATGCTGTCCTGCCGCCTCCAGTGCGCGCTGGCTGCGCTGTCCA24600.10393038523106979No Hit
GGATGTGAAAGTCTTCCAGAAGAAATTCTTGCAGCCAGCTTTG24570.10380364085883675No Hit
CCCCAGACTCCGTCAGTTTCTGCAGAAGTCCCTGGCTGCTGCC24450.10329666336990471No Hit
GTTCGGAGCCATCGCTGCTGCCTGCTGATCCGCGCCTAGAGTT24280.10257844526058431No Hit
GAGATGGGGTGTGGGGGCGAGGGATCAGAGGTCTGATATGGAC23770.10042379093262312No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA16350.021.837921
TTATGCG1201.0425538E-716.9583324
CGCAATA3350.016.01492536
CGAATTA700.002593581315.85714315
AAGACGG5300.015.7075475
CTTATAC13050.015.5938737
ATTAGAC1201.9375111E-615.4166663
TCTAGCG3600.015.41666628
CGAACGA2650.015.35849116
TTTAGAC1455.352922E-815.3103453
AGTGCGT855.367134E-415.2352948
TCTTATA19150.015.16710337
CAAGACG5750.015.1217394
TAACGAA2950.015.05084913
CTAGCGG3450.015.01449229
TACGACG3350.014.9104485
TCGACGT2750.014.829
ATCTCGT2900.014.67241437
GTACCAT1904.5656634E-1014.6052646
TATGCGG1406.002083E-714.5357155
Produced by FastQC (version 0.11.5)