FastQCFastQC Report
Fri 10 Feb 2017
ERR1630476.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630476.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1126615
Sequences flagged as poor quality0
Sequence length43
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTTAGGACACAGAGCACATCAAAAGTTCCCAAAGAGGGCTTG13500.11982798027720205No Hit
CTGTAGAACAGAGCAGGTGAAGAGAGAGCAAGCCCTCTTTGGG11680.10367339330649779No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCCCG451.3231896E-424.6666665
GTACTAG400.001931163723.1251
GGTATCA7100.020.3239441
CCGCTTA555.143008E-420.18181825
CTAGACT701.2191702E-418.54
TATAGAC701.2191702E-418.53
TAAGAGT801.6166243E-518.54
TATACTG1002.8748036E-718.4999985
TTACACT1156.40448E-817.6956524
TCTTATA8650.017.5375737
TAATACT1301.3929821E-817.0769234
GAGTTAG650.001579907317.0769231
TAGTACT1005.877582E-616.654
TCTAGCG1606.2937033E-1016.187528
CGCAATA1504.669346E-916.03333336
GCAGTCG2100.015.8571449
TATACCT1059.342688E-615.8571445
CGACCAT1751.3096724E-1015.85714310
GTACCTA700.002592451215.8571421
TCTATGG3050.015.7704912
Produced by FastQC (version 0.11.5)